CRISPR: Activity by EUN Partnership aisbl

Ana Lereno, Portugal

2018-03-26 15:31:34 UTC

1. Met
2. Met is only need to begin the amino acid sequence. Many proteins don't have this amino acid in the beginner of the sequence and for that, is remove.

Marzia, Italy

marzia_can — 2018-03-26 16:00:45 UTC

1- Met
2- Met can be cut after sinthesys

Lucia Italy

2018-03-26 16:03:39 UTC

1) Met
2) Met in the others sequences is remove

Cecilia Fontanella, Italy

ceciliafontanella — 2018-03-26 16:31:07 UTC

My answers are reported directly on the image below

Metionine

2018-03-26 17:41:21 UTC

Cause some proteins undergo a post-translation process in which this first aminoacid can be eliminated
*ATG TCT CCT AGT CAA(coding ADN)
*TAC AGA GGA TCA GTT
*AUG UCU CCU AGU CAA(mRNA)
*UAC AGA GGA UCA GUU(anticodon)
*Sequence of aminoacids in normal sequence:
Met-Ser-Pro-Ser-Gln
*Sequence in mutant:
AUGUGUCCUAGUCAA
Met-Cys-Pro-Ser_Gln

alfonsocanon — 2018-03-26 17:57:41 UTC

Alexandra, Portugal

2018-03-26 18:50:37 UTC

DNA (Ts) - TACAGAGGATCAGTT
mRNA (Codons) - AUGUCUCCUAGUCAA
tRNA(anticodons) - UACAGAGGAUCAGUU
Aminoacid
(1) Met - Ser - Pro - Ser - Gin
(2) Met - Ser - Pro - Cys - Gin
Mutant mRNA - AUGUCUCCUAGUCAA
Mutant DNA - ATGTCTCCTAGTCAA

Marjeta, Slovenia

marjeta_decman — 2018-03-26 19:13:57 UTC

1. Start codon encode Met.
2.

Carlo Italy

carlogino — 2018-03-26 19:18:32 UTC

Silvia, Italy

silvia_scandura — 2018-03-26 19:27:04 UTC

Questions:
1. The AUG start codon encodes for the amino acid Methionine
2. Some proteins are post-processed after encoding, so their starting amino acids are removed

theo_leftheroudis — 2018-03-26 19:47:10 UTC

I am so, sorry. I can’t do it, I don’t know anything about biology. An I don’ t Have time to learn now.

Marina del Barco SPAIN

marina_bm6 — 2018-03-26 19:52:20 UTC

CRISPR ACTIVITY
Questions:
1. The AUG start codon is always Methionine (Met).
2.Start codon could be removed after protein synthesis.
Activity:
DNA coding strand
ATG TCT CCT AGT CAA
DNA template strand
TAC AGA GGA TCA GTT
mRNA codons
AUG UCU CCU AGU CAA
tRNA anticodons
UAC AGA GGA UCA GUU
Amino acid sequence:
Normal PDSTB1
Met-Ser-Pro-Ser-Gln
Mutant PDSTB1
Met-Cys-Pro-Ser-Gln
Mutant mRNA
AUG UGU CCU AGU CAA
Mutant DNA coding strand
ATG TGT CCT AGT CAA

2018-03-26 20:44:26 UTC

Mayte, Spain

mayte_conejero — 2018-03-27 07:09:39 UTC

---TACAGAGGATCAGTT---

---AUG UCU CCU AGU CAA--

---UAC AGA GGA UCA GUU--

---Met-Ser-Pro-Ser-Gln---

---Met-Ser-Pro-Cys-Gln---

---AUG UCU CCU UGU CAA--

---TACAGAGGAACAGTT---

Maria A. - Italy

maria_alfano53 — 2018-03-27 08:39:19 UTC

Questions:
1. What amino acid does the start codon encode?
Methionine
2. Why don't all proteins have amino acid as their first?
The N-terminal methionine, although being the first amino acid, is not present at N-terminus of all proteins. This is because of a process that is known as post-translational modification. After a polypeptide is completely translated from mRNA, it is modified at different places by different enzymes, which are regulated by different (internal or external) factors.

Angela Italy

2018-03-27 10:58:22 UTC

Discovery

Gene therapy is an experimental technique for treating disease by altering the patient's genetic material. Most often, gene therapy works by introducing a healthy copy of a defective gene into the patient's cells. In recent years there have been rapid advancements in techniques that make it easier than ever to edit the human genome. Genome editing techniques, such as CRISPR/Cas9 allow scientists to edit the genome, by removing, replacing, or adding to parts of the DNA sequence.

Pilar, Spain

2018-03-27 11:09:49 UTC

1. Methionine.
2. Sometimes proteins suffer a maturation post-translational modification, that removes the first amino acid.

Nicola, Malta

nixy_port — 2018-03-27 13:08:42 UTC

1. Methionine
2. AUG is not the only start codon. Other codons may also initiate translation. Also methionine is not the N terminus for all proteins.

Francesco Piccolo, Italy

2018-03-27 13:33:12 UTC

1. AUG = Met (M);
2. Because it is not enough to have the beginning codon but other sequences are needed that allow the ribosomes to bind to it; among other things, it is not only the AUG (Methionine) codon that initiates the chain but has alternatives (CUG, UUG); finally, Methionine is often removed after the synthesis of some proteins.

Francesco Piccolo, Italy

2018-03-27 13:39:49 UTC

This is the screenshot, to comply with the delivery.

2018-03-27 16:15:36 UTC

Rossella Lupo, Italy

Estibaliz, Calgary

estivaz — 2018-03-27 17:02:36 UTC

1)ATG TCT CCT AGT CAA(coding ADN)
2)TAC AGA GGA TCA GTT
3)AUG UCU CCU AGU CAA(mRNA)
4)UAC AGA GGA UCA GUU
5)Sequence of aminoacids in normal sequence:
Met-Ser-Pro-Ser-Gln
6)Sequence in mutant:
AUGUGUCCUAGUCAA
Met-Cys-Pro-Ser_Gln

Ana, Spain

anamarialorentegarcia — 2018-03-27 19:22:35 UTC

Voinea Axinte Costica, Romania

2018-03-27 20:00:15 UTC

1. Start codon: AUG
2. Not all proteins have the first amino acid amino acid due to the maturation process of the molecules in which the first amino acid is usually removed, depending on the protein structure

2018-03-27 22:33:22 UTC

Genetic engineering is too difficult. I can not do this activity, I'm sorry.

Methionin, RNA AUG, DNA TAG, DNA codes many nonsense

2018-03-28 02:12:59 UTC

Alice, Italy

severi_alice — 2018-03-28 07:28:14 UTC

Katarina, Slovenia

katarina_brinovec — 2018-03-28 09:00:09 UTC

1. (DNA coding) :
..ATG TCT CCT AGT CAA...
2. (DNA template)
...TAC AGA GGA TCA GTT...
3.(mRNA codons)
...AUG UCU CCU AGU CAA...
4. (tRNA anticodons)
...UAC AGA GGA UCA GUU...
5. (Aminoacids sequence- normal)
Met-Ser-Pro-Ser-Gln
6.( Aminoacids sequence-mutant)
Met-Cys-Pro-Ser-Gln
7.(Mutant mRNA)
...AUG-UGU-CCU-AGU-CAA...
8. (DNA template)
...TAC ACA-GGA-TCA-GTT...
9.. (Mutant DNA coding)
...ATG-TGT-CCT-AGT-CAA...
10. Met
11. Because of post - translation chamodification.

katarina_brinovec — 2018-03-28 09:07:32 UTC

Sequence of aminoacids in normal sequence:

katarina_brinovec — 2018-03-28 09:10:21 UTC

in normal

Natalia Grushko, Ukraine

2018-03-28 10:11:53 UTC

2) TAC AGA GGA TCA GTT
3) AUG TCU CCU AGU CAA
4) UAC UGA GGA UCA GUU
For me it was very interesting, but I have to asked my students about help.

michelapellizzari4 — 2018-03-28 10:32:24 UTC

Michela, Italy

michelapellizzari4 — 2018-03-28 10:48:30 UTC

Sonila,Albania

2018-03-28 13:03:54 UTC

A little help nedded though

2018-03-28 13:04:42 UTC

collabscience — 2018-03-28 15:56:36 UTC

Michael, Ireland

nenadic_ivana007 — 2018-03-28 16:54:28 UTC

DNA Coding strand: ATG TCT CCT AGT CAADNA Template strand: TAC AGA GGA TCA GTTmRNA Codons: AUG UCU CCU AGU CAAtRNA Anticodons: UAC ACA GGA UCA GUUAmino Acid Sequence: Normal PDSTß1: Met Ser Pro Ser Gln Mutant PDSTß1: Met Cys Pro Ser GlnMutant mRna: AUG UGU CCU AGU CAAMutant DNA coding strand ATG TGT CCT AGT CAA1. Met

2018-03-28 16:59:44 UTC

Gilda/Italy

gilda_fiengo — 2018-03-28 18:01:15 UTC

Mustafa BOZOGLAN, Turkey

mdbozoglan — 2018-03-28 19:07:14 UTC

aysegulmer — 2018-03-29 07:00:37 UTC

Ayşegül/Turkey

Lidia , Ro

2018-03-29 10:23:52 UTC

Very interesting...

Maria DC, Italy

mariadecarlo — 2018-03-29 13:05:51 UTC

Being a professor of religion, and collaborating with my science colleague, I was helped to carry out this exercise and to understand that:
1. Which amino acid encodes the start of the codon?
methionine
2. Why do not all proteins have amino acids as before?
N-terminal methionine, although it is the first amino acid, is not present in the N-terminus of all proteins. This is due to a process known as post-translational modification. After a polypeptide has been completely translated by mRNA, it is modified at different points by different enzymes, which are regulated by different factors (internal or external).

Anna Italy

annagrignetti — 2018-03-29 13:06:26 UTC

Working together with my religion colleague, we have arrived at these conclusions.
1. Which amino acid encodes the codon beginning?
methionine
2. Why do not all proteins have amino acids as before?
N-terminal methionine, although it is the first amino acid, is not present in the N-terminus of all proteins. This is due to a process known as post-translational modification. After a polypeptide has been completely translated by mRNA, it is modified at different points by different enzymes, which are regulated by different factors (internal or external).

Vesna, Slovenia

2018-03-29 14:33:18 UTC

Tina, Austria

2018-03-29 14:40:12 UTC

Daria, Poland

2018-03-29 15:18:17 UTC

DNA Coding strand: ATG TCT CCT AGT CAA
DNA Template strand: TAC AGA GGA TCA GTT
mRNA Codons: AUG UCU CCU AGU CAA
tRNA Anticodons: UAC AGA GGA UCA GUU
Normal PDST: Met Ser Pro Ser Gln
Mutant: Met Cys Pro Ser Gln
mutant mRNA:AUG UGU CCU AGU CAA
Mutant DNA: ATG TGT CCT AGT CAA
1. Met

Sophie, Austria

2018-03-29 15:26:46 UTC

DNA Coding strand:
--- A T G T C T C C T A G T C A A ---

DNA Template strand:
--- T A C A G A G G A T C A G T T ---

mRNA with codons:
--- A U G U C U C C U A G U C A A ---

tRNA with anti-codons:
--- U A C A G A G G A U C A G U U ---

Normal PDSTβ1:
--- Met Ser Pro Ser Gln ---
Mutant PDSTβ1:
--- Met Cys Pro Ser Gln ---
Mutant mRNA:
--- A U G U G U C C U A G U C A A ---
Mutant DNA:
--- A T G T G T C C T A G T C A A ---

1. What amino acid does the start codon encode? Methionine.

2. Why don’t all proteins have this amino acid as their first? because of posttranslational modification

Chiara G. Italy

chiara_garulli — 2018-03-29 17:21:01 UTC

DNA Coding Strand
--ATG-TCT-CCT-AGT-CAA--
DNA Template
--TCA-AGA-GGA-TCA-GTT--
mRNA CODONS
--AUG-UCU-CCU-AGU-CAA--
tRNA ANTICODONS
--UAC-AGA-GGA-UCA-GUU--

PROTEIN
NORMAL
--Met-Ser*-Pro-Ser-Gln--
MUTANT
--Met-Cys*-Pro-Ser-Gln--

MUTANT mRNA
--AUG-UG*U-CCU-AGU-CAA-
MUTANT DNA CODING STRAND
--ATG-TG*T-CCT-AGT-CAA--

Tanja, Austria

2018-03-29 18:06:49 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA
DNA Template strand:
TAC AGA GGA TCA GTT
mRNA Codons:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU
Normal PDSTβ1 :
Met Ser Pro Ser Gln
Mutant PDSTβ1:
Met Cys Pro Ser Gln
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA Coding strand:
ATG TGT CCT AGT CAA

Questions:
1) Met
2) Because of post-translational modification

Rishu, India

2018-03-30 05:44:56 UTC

ATG-TCT-CCT-AGT-CCA
TAC-AGA-GGA-TCA-GTT
mRNA CODON
AUG-UCU-CCU-AGU-CAA
tRNA anticodon
UAC-AGA-GGA-UCA-GUU
Normal PDS..
Met-ser-pro-ser-gln
Mutant mRNA
AUG-UGU-CCU-AGU-CAA
Mutant PDS
Met-Cys-Pro-Ser-gln
Mutant DNA
ATG-TGT-CCT-AGT-CAA

Inmaculada González/Spain

ingoalster — 2018-03-30 06:34:20 UTC

I have been trying tounderstand this activity but I am affraid that I don't know to solve it. I am sorry.

Paola, italy

paolacannadabartoli — 2018-03-30 09:04:51 UTC

Shirin, Azerbaijan

shirin_sultan — 2018-03-30 09:28:30 UTC

ATG-TCT-CCT-AGT-CAA
TAC-ACA-GGA-TCA-GTT
mRNA
AUGUCUCCUAGUCAA
tRNA
UACAGAGGAUCAGUU
Normal PDSTB1
Met-Ser-Pro-SEr-Gln
Mutant PDSTB1
Met-Cys-Pro-Ser-Gln
Mutant DNA
ATG-TGT-CCT-ACT-CAA
Mutant RNA
AUG-UGU-CCU-AGU-CAA

Petros, Greece

2018-03-30 09:36:06 UTC

too time consuming as I am not on this field.
Sorry

Cristina, Spain

madrastra6767 — 2018-03-30 10:40:49 UTC

I am not from this field either. So, I have some problems to solve this activity

Rosy,

2018-03-30 11:29:28 UTC

Rosy, Italy

2018-03-30 11:29:40 UTC

I teach in the Primary school. I am sorry but it's not from the field and I don not know how to solve this activity.

Silvia, Spain

smaymo — 2018-03-30 11:34:47 UTC

1. Methionine
2. There are otheer codons to initiate the synthesis, particularly in Prokaryotes, like UUG

Paulo Dionisio, Portugal

paulo_dionisio — 2018-03-30 14:29:30 UTC

I think this activity is very specific for a certain science.
I teach Mathematics, so it's not my field of work and I do not know how to solve this activity. I tried to understand and do online research to solve the problem, but I'm afraid the answers iI found might not be correct.

Anca, Romania

2018-03-30 15:15:45 UTC

Olga, Republic of Moldova

olgagolovetchi — 2018-03-30 16:31:04 UTC

I am a math teacher so I have no idea about this .

anita_simac — 2018-03-30 19:27:36 UTC

DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Normal PDSTβ1 : Met Ser Pro Ser Gln Mutant PDSTβ1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Questions: 1) Methionin 2) All proteins don’t have this amino acid as their first because of posttranslational modification.

Anita, Croatia

anita_simac — 2018-03-30 19:28:32 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA
DNA Template strand:
TAC AGA GGA TCA GTT
mRNA Codons:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU
Normal PDSTβ1
:
Met Ser Pro Ser Gln
Mutant PDSTβ1:
Met Cys Pro Ser Gln
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA Coding
strand:
ATG TGT CCT AGT CAA

Questions:
1) Methionin
2) All proteins don’t
have this amino acid as their first because of posttranslational modification.

Marina, Italy

2018-03-31 08:30:44 UTC

I am Biologist of National Order, I have created and realized with high school students these activities, bioenglish research activities presented also in Workshop for teacher as relator about modern biology , also about CRISPR

Viktorija, Slovenia

2018-03-31 12:39:45 UTC

This activity is too difficult for me because I am a mathematics teacher and I have no idea what to do. I can only share notes with you about the theme.

Orsola, Italy

orsola_parisi58 — 2018-03-31 15:40:46 UTC

DNA strand:
ATG TCT CCT AGT CAA
DNA Template:
TAC AGA GGA TCA GTT
MRNA codes:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU
PDSTβ1 normal:
Met Ser Pro Ser Gln
PDSTβ1 mutant:
Met Cys Pro Ser Gln
MRNA mutant:
AUG UGU CCU AGU CAA
Filament Mutant DNA Coding:
ATG TGT CCT AGT CAA

Questions:
1) Met
2) because methionine is removed after protein synthesis

Nina, Slovenija

2018-03-31 15:43:21 UTC

Helen Mihalatou, Greece

helensefer — 2018-03-31 15:57:46 UTC

Answers

1. Methionine.

2. Because sometimes polypeptide chains may undergo post-translational modification to form the mature protein product.

Laurence, Italy

2018-03-31 17:19:22 UTC

Tanja, Slovenia

brinje_raziskovalci — 2018-03-31 19:55:11 UTC

JGpe, México

2018-03-31 21:25:00 UTC

Good

Sevi, Greece

sevasti — 2018-04-01 07:11:59 UTC

Although it seems interesting, quite difficult for a physicist..

Poonam, Ind

2018-04-01 10:19:56 UTC

sunce

suncezgb8 — 2018-04-01 12:09:16 UTC

Doinita B., Romania

2018-04-01 17:59:37 UTC

Interesting, but difficult for me ...

Noel, Malta

noel_harmsworth — 2018-04-01 20:23:12 UTC

Had to do some research for this one. Here are my workings:

Barbara, Poland

2018-04-01 22:46:42 UTC

1. What amino acid does the start codon encode?
methionine
2. Why don't all proteins have this amino acid as their first?
The first amino acid in the protein depends on the start codon, which is different.
Therefore, the first amino acid in the protein can also be different.
E.g:
- in Prokaryote, the codon Start is AUG, coding for formylmethionine,
- for some Prokaryotes there is an alternative Start GUG codon encoding valine,
- at Eukaryota, the codon Start is AUG, coding for methionine.
Are the start codons in mRNA in the mitochondria and chloroplasts different, I don't know.

Pinelopi, Greece

2018-04-02 07:50:47 UTC

Interesting but quite difficult for a teacher...

Juanjo /Spain

2018-04-02 15:02:15 UTC

1. What amino acid does the start condon encode?
methionine
2. Why don't all proteins have the amino acid as their first?
I'm not sure, but it would be remove after synthesis.

Iuliana, Romania

aurelian_iuliana — 2018-04-02 18:00:14 UTC

I didn't know that I am a genetic engineer...:) I thought that I'm a physics teacher. But challenge accepted!

,1. Met (methionine)
2. I suppose that there are many other proteins and their codons don't necessary start with AUG...
Target codon is closer to the start codon, so the solution would be the 2nd one.

Barbara, Poland

2018-04-03 06:17:08 UTC

Continued answer to question 2:
Post-translational modification of proteins: proteases remove unnecessary fragments, various functional groups are added.

chemingui firas nabeul Tunisia

cheminguifiras1966 — 2018-04-03 10:44:02 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA
DNA Template strand:
TAC AGA GGA TCA GTT
mRNA Codons:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU

Normal PDSTβ1
Met Ser Pro Ser Gln

Mutant PDSTβ1:
Met Cys Pro Ser Gln
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA Coding strand:
ATG TGT CCT AGT CAA

Answers:
1) Methionin
2) All
proteins don’t have this amino acid because of the introduced single point mutation, the sequence of the coding DNA strand can be altered to bring about the required change in protein can alter and change

Beate, Austria

2018-04-03 12:31:32 UTC

1. Methionine
2. Because it has been removed after synthesis

Alexia, Malta

2018-04-03 16:25:01 UTC

· DNA Coding strand: ATG TCT CCT AGT CAA

· DNA Template strand: TAC AGA GGA TCA GTT

· mRNA Codons: AUG UCU CCU AGU CAA

· tRNA Anticodons: UAC AGA GGA UCA GUU

· Normal PDSTβ1 : Met Ser Pro Ser Gln

· Mutant PDSTβ1: Met Cys Pro Ser Gln

· Mutant mRNA: AUG UGU CCU AGU CAA

· Mutant DNA Coding strand: ATG TGT CCT AGT CAA

· Questions:

1. Methionin

2. Methionin is removed after protein synthesis thus not all proteins start with it

Natalia, Spain

2018-04-03 16:29:42 UTC

I am an English teacher and a Natural Sciences teacher in Primary Education.
I don´t
understand the activity

Kavita, India

2018-04-04 04:39:51 UTC

Hi. I did find the activity tough but it was a great learning experience. Moreover, I looked at all my fellow colleagues answers to check my responses. Few answers I could not attempt as I could not understand.

Anna, Italy

prof_deruvo — 2018-04-04 08:21:27 UTC

I’m not sure it is correct. About the last question I don’t know what answer. The Met codon is a start sign too but what is the mechanism why some time is read as start sign and some time as Met?

Sonja, Croatia

sonja_pospisil77 — 2018-04-04 09:20:55 UTC

DNA strand:
ATG TCT CCT AGT CAA
DNA Template:
TAC AGA GGA TCA GTT
mRNA codes:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU
Amino acid sequence:
normal PDSTβ1:
Met Ser Pro Ser Gln
mutant PDSTβ1 :
Met Cys Pro Ser Gln
mutant mRNA :
AUG UGU CCU AGU CAA
mutant DNA Coding strand:
ATG TGT CCT AGT CAA

Questions:
1. Met
2. All proteins don't have the Met amino acid as their first because of the mutation.

sonja_pospisil77 — 2018-04-04 09:22:12 UTC

normal

sonja_pospisil77 — 2018-04-04 09:22:29 UTC

PDSTβ1

Dorothea, Vienna

a00902838 — 2018-04-04 11:04:13 UTC

DNA Coding strand:

ATG TCT CCT AGT CAA

DNA template strand:

TAC AGA GGA TCA GTT

mRNA codons:

AUG UCU CCU AGU CAA

tRNA Anticodons:

UAC AGA GGA UCA GUU

Amino acid sequence normal PDSTß1:

Met Ser Pro Ser Gln

Amino acid sequence mutated PDSTß1:

Met Cys Pro Ser Gln

Mutant mRNA:

AUG UGU CCU AGU CAA

Mutant DNA Coding strand:

ATG TGT CCT AGT CAA

1. Start codon = AUG = Methionine

2. Because bacteria can use the alternative start codons GUG und UUG besides AUG.

Anamaria, Romania

2018-04-04 11:42:31 UTC

Philipp, Austria

2018-04-04 15:23:54 UTC

I hope you can read it^^ :)

Bozena,Poland

2018-04-04 19:00:23 UTC

unfortunatelly I have forgotten how to do this exercise. To difficult for me.

Slavica, Croatia

ps_slavica — 2018-04-04 19:07:06 UTC

uff... DNA... biology... not my field...

Zhelyazka, Bulgaria

janeraikova — 2018-04-04 19:28:42 UTC

I am a physicist and this is a difficult exercise for me.

MaríaJosé, Spain

2018-04-04 20:43:48 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA
DNA template strand:
TAC AGA GGA TCA GTT
mRNA codons:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU
Amino acid sequence normal PDSTß1:
Met Ser Pro Ser Gln
Amino acid sequence mutated PDSTß1:
Met Cys Pro Ser Gln
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA Coding strand:
ATG TGT CCT AGT CAA

Question 1: Methionine,
Question 2: Start codon AUG also codes for methionine and without start codon translation does not happen. And even the ambiguous codon GUG codes for methionine when it is first. So does this mean that all proteins start with methionine as the first amino acid.

Klara,Austria

2018-04-05 08:42:48 UTC

Zvonko, Serbia

gzvonko62 — 2018-04-05 11:12:58 UTC

Since I am an electrical engineer, this is difficult exercise for me.

2018-04-05 12:15:37 UTC

Kerstin, Austria

2018-04-05 16:01:54 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA

DNA Template strand:
TAC AGA GGA TCA GTT

mRNA codons:
AUG UCU CCU AGU CAA

tRNA Anticodons:
UAC AGA GGA UCA GUU

Amino acid sequence Normal PDSTß1:
Met Ser Pro Ser Gln

Amino acid sequence Mutated PDSTß1:
Met Cys Pro Ser Gln

Mutant mRNA:
AUG UGU CCU AGU CAA

Mutant DNA Coding strand:
ATG TGT CCT AGT CAA

Grażyna, Poland

sp171wesola — 2018-04-05 19:03:36 UTC

I don't think I can do it at the moment. I need to lean a lot....

Bettencourt, Portugal

efsantos — 2018-04-05 22:31:59 UTC

hummm ... i must review my biochemistry lessons!!! I can match DNA and RNA sequences but use the Wheel!!!!!...
Q1- after webconsulting , start codon= Met (AUG)
Q2 - ?????

Bhagyashree S, India

bhagyashree_semlani — 2018-04-06 07:03:29 UTC

I think I have to take help of my head to solve this. This seems too difficult for me. I need to learn alot.

Ayşe Arslan,Turkey

aysekaraul — 2018-04-06 09:00:09 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA
DNA template strand:
TAC AGA GGA TCA GTT
mRNA codons:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU
Amino acid sequence normal PDSTß1:
I'm not sure.
Amino acid sequence mutated PDSTß1:
I'm not sure.
Mutant mRNA:
I'm not sure.
Mutant DNA Coding strand:
I'm not sure.

A 1: Met
A 2: Because, start codon AUG.

Kristina, Croatia

2018-04-06 11:02:08 UTC

Zdena, CR

2018-04-06 11:30:12 UTC

Miguel Angel, Burgos (Spain)

kapy83 — 2018-04-06 15:51:47 UTC

Enrique, Segovia

2018-04-07 14:15:02 UTC

Cristina Raposo, Portugal

ciraposo — 2018-04-07 18:06:17 UTC

Laura, Italy

2018-04-08 06:47:42 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA
DNA Template strand:
TAC AGA GGA TCA GTT
mRNA Codons:
AUG UCU CCU AGU CAA
tRNA Anticodons:
UAC AGA GGA UCA GUU
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA Coding strand:
ATG TGT CCT AGT CAA

Question 1: Methionine

Ricardo RG

RicardoRG — 2018-04-08 14:28:26 UTC

Joyce, India

sec_coordinator — 2018-04-08 18:12:33 UTC

2018-04-08 19:48:46 UTC

Dorota, Poland

Justyna,Poland

2018-04-08 21:14:54 UTC

DNA Coding atarted: ATG TCT CCT AGT CAA
DNA Template started: TAC AGA GGA TCA GTT
mRNA Codons: AUG UCU CCU AGU CAA
tRNA Anticodons: UAC AGA GGA UCA GUU
Amino acid sequence:
Normal PDST beta1: Met Ser, Pro, Ser, Gln
Mutant PDST beta 1: Met, Cys, Pro, Ser, Gln
Mutant mRNA: AUG, UGU, CCU, AGU, CAA
Mutant DNA Coding strand: ATG TGT CCT AGT CAA
Answer Questions:
1. Met
2. becouse Met is removed after protein synthesis
PS.
My friend biology teacher help me, i didnt understand this experience here. It was ununderstnding

Fiona

2018-04-09 16:15:43 UTC

Start codon is Met. I found this task difficult as i have never used this type of wheel before and didn't fully understand how to use it.

Nada, Macedonia

2018-04-09 17:43:20 UTC

Nada, Macedonia

2018-04-09 17:54:56 UTC

Konstantin, Vienna

2018-04-09 18:02:56 UTC

DNA coding strand:
ATG TCT CCT AGT CAA
DNA template strand:
TAC AGA GGA TCA GTT
mRNA codons:
AUG UCU CCU AGU CAA
tRNA anticodons:
UAC AGA GGA UCA GAA
AA sequence wt:
Met Ser Pro Ser Gln
AA sequence mut:
Met Cys Pro Ser Gln
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA cds:
ATG TGT CCT AGT CAA

The first codon codes for the aa methionine.
While all start codons (even GUG or UUG) code for methionine or fMet, due to post transcriptional processing of the proteins the N-terminal aa may actually not be the first in the final protein.

2018-04-10 10:40:26 UTC

Bertha, México

2018-04-10 17:47:42 UTC

I have some answers pending that I do not know at the moment. I will investigate and share again.

Sandra, Portugal

SSLeal — 2018-04-11 23:32:24 UTC

DNA coding strans: ATGTCTCCTAGTCAA;
DNA template strand: TACAGAGGATCAGTT;
mRNA codons: AUG; UCU; CCU; AGU; CAA
tRNA anticodons: UAC; AGA; GGA; UCA; GUU
Amino acid sequence normal: Met; Ser; Pro; Ser; Gln
Amino acid sequence mutant: Met; Cys; Pro; Ser; Gln
Mutant mRNA: AUG; UGU; CCU; AGU; CAA
Mutant DNA coding strand: ATGTGTCCTAGTCAA
Answer questions:
1- Met - methionine

Dimitris,Greece

tsiastoudis — 2018-04-12 14:44:46 UTC

DNA template:
TAC AGA GGA TCA GTT
mRNA:
AUG UCU CCU AGU CAA
tRNA:
UAC AGA GGA UCA GUU
Amino acid normal:
Met Ser Pro Ser Gln
Amino acid mutated:
Met Cys Pro Ser Gln
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA:
ATG TGT CCT AGT CAA
1)Methionin 2)It is removed

Špela, Slovenia

rozman_spela — 2018-04-12 18:07:09 UTC

I don't know the answer to the last question and am not interested enough to search for an answer, sory. The task was intesting, I did learn a lot about DNA and I finaly understand what the Cryser method is. But enough of that please, I'm a physicist :)

Aoife, Ireland

2018-04-12 20:01:07 UTC

Aneta, Polan

2018-04-13 13:42:26 UTC

Jasenka, Croatia

2018-04-14 13:25:44 UTC

aurelian_iuliana — 2018-04-14 16:51:20 UTC

Jasenka, Croatia Jasenka, Croatia Aneta, Polan Aneta, Polan Aoife, Ireland Aoife, Ireland Špela, Slovenia Špela, Slovenia I don't know the answer to the last question and am not interested enough to search for an answer, sory. The task was intesting, I did learn a lot about DNA and I finaly understand what the Cryser method is. But enough of that please, I'm a physicist :) Dimitris,Greece Dimitris,Greece DNA template: TAC AGA GGA TCA GTT mRNA: AUG UCU CCU AGU CAA tRNA: UAC AGA GGA UCA GUU Amino acid normal: Met Ser Pro Ser Gln Amino acid mutated: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA: ATG TGT CCT AGT CAA 1)Methionin 2)It is removed Sandra, Portugal Sandra, Portugal DNA coding strans: ATGTCTCCTAGTCAA; DNA template strand: TACAGAGGATCAGTT; mRNA codons: AUG; UCU; CCU; AGU; CAA tRNA anticodons: UAC; AGA; GGA; UCA; GUU Amino acid sequence normal: Met; Ser; Pro; Ser; Gln Amino acid sequence mutant: Met; Cys; Pro; Ser; Gln Mutant mRNA: AUG; UGU; CCU; AGU; CAA Mutant DNA coding strand: ATGTGTCCTAGTCAA Answer questions: 1- Met - methionine Bertha, México Bertha, México I have some answers pending that I do not know at the moment. I will investigate and share again. 📎 Photo Nada, Macedonia Nada, Macedonia Nada, Macedonia Nada, Macedonia Fiona Fiona Start codon is Met. I found this task difficult as i have never used this type of wheel before and didn't fully understand how to use it. Konstantin, Vienna Konstantin, Vienna DNA coding strand: ATG TCT CCT AGT CAA DNA template strand: TAC AGA GGA TCA GTT mRNA codons: AUG UCU CCU AGU CAA tRNA anticodons: UAC AGA GGA UCA GAA AA sequence wt: Met Ser Pro Ser Gln AA sequence mut: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA cds: ATG TGT CCT AGT CAA The first codon codes for the aa methionine. While all start codons (even GUG or UUG) code for methionine or fMet, due to post transcriptional processing of the proteins the N-terminal aa may actually not be the first in the final protein. Justyna,Poland Justyna,Poland DNA Coding atarted: ATG TCT CCT AGT CAA DNA Template started: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Amino acid sequence: Normal PDST beta1: Met Ser, Pro, Ser, Gln Mutant PDST beta 1: Met, Cys, Pro, Ser, Gln Mutant mRNA: AUG, UGU, CCU, AGU, CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Answer Questions: 1. Met 2. becouse Met is removed after protein synthesis PS. My friend biology teacher help me, i didnt understand this experience here. It was ununderstnding Dorota, Poland Dorota, Poland Joyce, India Joyce, India Ricardo RG Ricardo RG Laura, Italy Laura, Italy DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Question 1: Methionine Enrique, Segovia Enrique, Segovia Miguel Angel, Burgos (Spain) Miguel Angel, Burgos (Spain) Zdena, CR Zdena, CR Cristina Raposo, Portugal Cristina Raposo, Portugal Kristina, Croatia Kristina, Croatia Ayşe Arslan,Turkey Ayşe Arslan,Turkey DNA Coding strand: ATG TCT CCT AGT CAA DNA template strand: TAC AGA GGA TCA GTT mRNA codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Amino acid sequence normal PDSTß1: I'm not sure. Amino acid sequence mutated PDSTß1: I'm not sure. Mutant mRNA: I'm not sure. Mutant DNA Coding strand: I'm not sure. A 1: Met A 2: Because, start codon AUG. Bhagyashree S, India Bhagyashree S, India I think I have to take help of my head to solve this. This seems too difficult for me. I need to learn alot. Bettencourt, Portugal Bettencourt, Portugal hummm ... i must review my biochemistry lessons!!! I can match DNA and RNA sequences but use the Wheel!!!!!... Q1- after webconsulting , start codon= Met (AUG) Q2 - ????? Grażyna, Poland Grażyna, Poland I don't think I can do it at the moment. I need to lean a lot.... Kerstin, Austria Kerstin, Austria DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Amino acid sequence Normal PDSTß1: Met Ser Pro Ser Gln Amino acid sequence Mutated PDSTß1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA 📎 Photo Zvonko, Serbia Zvonko, Serbia Since I am an electrical engineer, this is difficult exercise for me. Klara,Austria Klara,Austria MaríaJosé, Spain MaríaJosé, Spain DNA Coding strand: ATG TCT CCT AGT CAA DNA template strand: TAC AGA GGA TCA GTT mRNA codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Amino acid sequence normal PDSTß1: Met Ser Pro Ser Gln Amino acid sequence mutated PDSTß1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Question 1: Methionine, Question 2: Start codon AUG also codes for methionine and without start codon translation does not happen. And even the ambiguous codon GUG codes for methionine when it is first. So does this mean that all proteins start with methionine as the first amino acid. Zhelyazka, Bulgaria Zhelyazka, Bulgaria I am a physicist and this is a difficult exercise for me. Slavica, Croatia Slavica, Croatia uff... DNA... biology... not my field... Bozena,Poland Bozena,Poland unfortunatelly I have forgotten how to do this exercise. To difficult for me. Philipp, Austria Philipp, Austria I hope you can read it^^ :) Anamaria, Romania Anamaria, Romania Dorothea, Vienna Dorothea, Vienna DNA Coding strand: ATG TCT CCT AGT CAA DNA template strand: TAC AGA GGA TCA GTT mRNA codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Amino acid sequence normal PDSTß1: Met Ser Pro Ser Gln Amino acid sequence mutated PDSTß1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA 1. Start codon = AUG = Methionine 2. Because bacteria can use the alternative start codons GUG und UUG besides AUG. PDSTβ1 PDSTβ1 normal normal Anna, Italy Anna, Italy I’m not sure it is correct. About the last question I don’t know what answer. The Met codon is a start sign too but what is the mechanism why some time is read as start sign and some time as Met? Kavita, India Kavita, India Hi. I did find the activity tough but it was a great learning experience. Moreover, I looked at all my fellow colleagues answers to check my responses. Few answers I could not attempt as I could not understand. Sonja, Croatia Sonja, Croatia DNA strand: ATG TCT CCT AGT CAA DNA Template: TAC AGA GGA TCA GTT mRNA codes: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Amino acid sequence: normal PDSTβ1: Met Ser Pro Ser Gln mutant PDSTβ1 : Met Cys Pro Ser Gln mutant mRNA : AUG UGU CCU AGU CAA mutant DNA Coding strand: ATG TGT CCT AGT CAA Questions: 1. Met 2. All proteins don't have the Met amino acid as their first because of the mutation. Natalia, Spain Natalia, Spain I am an English teacher and a Natural Sciences teacher in Primary Education. I don´t understand the activity Alexia, Malta Alexia, Malta · DNA Coding strand: ATG TCT CCT AGT CAA · DNA Template strand: TAC AGA GGA TCA GTT · mRNA Codons: AUG UCU CCU AGU CAA · tRNA Anticodons: UAC AGA GGA UCA GUU · Normal PDSTβ1 : Met Ser Pro Ser Gln · Mutant PDSTβ1: Met Cys Pro Ser Gln · Mutant mRNA: AUG UGU CCU AGU CAA · Mutant DNA Coding strand: ATG TGT CCT AGT CAA · Questions: 1. Methionin 2. Methionin is removed after protein synthesis thus not all proteins start with it Beate, Austria Beate, Austria 1. Methionine 2. Because it has been removed after synthesis Barbara, Poland Barbara, Poland Continued answer to question 2: Post-translational modification of proteins: proteases remove unnecessary fragments, various functional groups are added. Iuliana, Romania Iuliana, Romania 1. Met (methionine) 2. I suppose that there are many other proteins and their codons don't necessary start with AUG... Target codon is close to the start codon, so the solution would be the 2nd one. Juanjo /Spain Juanjo /Spain 1. What amino acid does the start condon encode? methionine 2. Why don't all proteins have the amino acid as their first? I'm not sure, but it would be remove after synthesis. Pinelopi, Greece Pinelopi, Greece Interesting but quite difficult for a teacher... chemingui firas nabeul Tunisia chemingui firas nabeul Tunisia DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Normal PDSTβ1 Met Ser Pro Ser Gln Mutant PDSTβ1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Answers: 1) Methionin 2) All proteins don’t have this amino acid because of the introduced single point mutation, the sequence of the coding DNA strand can be altered to bring about the required change in protein can alter and change Barbara, Poland Barbara, Poland 1. What amino acid does the start codon encode? methionine 2. Why don't all proteins have this amino acid as their first? The first amino acid in the protein depends on the start codon, which is different. Therefore, the first amino acid in the protein can also be different. E.g: - in Prokaryote, the codon Start is AUG, coding for formylmethionine, - for some Prokaryotes there is an alternative Start GUG codon encoding valine, - at Eukaryota, the codon Start is AUG, coding for methionine. Are the start codons in mRNA in the mitochondria and chloroplasts different, I don't know. Noel, Malta Noel, Malta Had to do some research for this one. Here are my workings: Doinita B., Romania Doinita B., Romania Interesting, but difficult for me ... sunce sunce Poonam, Ind Poonam, Ind DNA strand: ATG TCT CCT AGT CAA DNA Template: TAC AGA GGA TCA GTT MRNA codes: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU PDSTβ1 normal: Met Ser Pro Ser Gln PDSTβ1 mutant: Met Cys Pro Ser Gln MRNA mutant: AUG UGU CCU AGU CAA Filament Mutant DNA Coding: ATG TGT CCT AGT CAA Questions: 1) Met 2) because methionine is removed after protein synthesis Sevi, Greece Sevi, Greece Although it seems interesting, quite difficult for a physicist.. JGpe, México JGpe, México Good Tanja, Slovenia Tanja, Slovenia Laurence, Italy Laurence, Italy Helen Mihalatou, Greece Helen Mihalatou, Greece Answers 1. Methionine. 2. Because sometimes polypeptide chains may undergo post-translational modification to form the mature protein product. Nina, Slovenija Nina, Slovenija Orsola, Italy Orsola, Italy DNA strand: ATG TCT CCT AGT CAA DNA Template: TAC AGA GGA TCA GTT MRNA codes: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU PDSTβ1 normal: Met Ser Pro Ser Gln PDSTβ1 mutant: Met Cys Pro Ser Gln MRNA mutant: AUG UGU CCU AGU CAA Filament Mutant DNA Coding: ATG TGT CCT AGT CAA Questions: 1) Met 2) because methionine is removed after protein synthesis Viktorija, Slovenia Viktorija, Slovenia This activity is too difficult for me because I am a mathematics teacher and I have no idea what to do. I can only share notes with you about the theme. Marina, Italy Marina, Italy I am Biologist of National Order, I have created and realized with high school students these activities, bioenglish research activities presented also in Workshop for teacher as relator about modern biology , also about CRISPR Anita, Croatia Anita, Croatia DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Normal PDSTβ1 : Met Ser Pro Ser Gln Mutant PDSTβ1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Questions: 1) Methionin 2) All proteins don’t have this amino acid as their first because of posttranslational modification. DNA Coding strand: ATG T DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Normal PDSTβ1 : Met Ser Pro Ser Gln Mutant PDSTβ1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Questions: 1) Methionin 2) All proteins don’t have this amino acid as their first because of posttranslational modification. Olga, Republic of Moldova Olga, Republic of Moldova I am a math teacher so I have no idea about this . Anca, Romania Anca, Romania Paulo Dionisio, Portugal Paulo Dionisio, Portugal I think this activity is very specific for a certain science. I teach Mathematics, so it's not my field of work and I do not know how to solve this activity. I tried to understand and do online research to solve the problem, but I'm afraid the answers iI found might not be correct. Silvia, Spain Silvia, Spain 1. Methionine 2. There are otheer codons to initiate the synthesis, particularly in Prokaryotes, like UUG Rosy, Rosy, Rosy, Italy Rosy, Italy I teach in the Primary school. I am sorry but it's not from the field and I don not know how to solve this activity. Cristina, Spain Cristina, Spain I am not from this field either. So, I have some problems to solve this activity Petros, Greece Petros, Greece too time consuming as I am not on this field. Sorry Paola, italy Paola, italy Inmaculada González/Spain Inmaculada González/Spain I have been trying tounderstand this activity but I am affraid that I don't know to solve it. I am sorry. Shirin, Azerbaijan Shirin, Azerbaijan ATG-TCT-CCT-AGT-CAA TAC-ACA-GGA-TCA-GTT mRNA AUGUCUCCUAGUCAA tRNA UACAGAGGAUCAGUU Normal PDSTB1 Met-Ser-Pro-SEr-Gln Mutant PDSTB1 Met-Cys-Pro-Ser-Gln Mutant DNA ATG-TGT-CCT-ACT-CAA Mutant RNA AUG-UGU-CCU-AGU-CAA Rishu, India Rishu, India ATG-TCT-CCT-AGT-CCA TAC-AGA-GGA-TCA-GTT mRNA CODON AUG-UCU-CCU-AGU-CAA tRNA anticodon UAC-AGA-GGA-UCA-GUU Normal PDS.. Met-ser-pro-ser-gln Mutant mRNA AUG-UGU-CCU-AGU-CAA Mutant PDS Met-Cys-Pro-Ser-gln Mutant DNA ATG-TGT-CCT-AGT-CAA Tanja, Austria Tanja, Austria DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Normal PDSTβ1 : Met Ser Pro Ser Gln Mutant PDSTβ1: Met Cys Pro Ser Gln Mutant mRNA: AUG UGU CCU AGU CAA Mutant DNA Coding strand: ATG TGT CCT AGT CAA Questions: 1) Met 2) Because of post-translational modification Chiara G. Italy Chiara G. Italy DNA Coding Strand --ATG-TCT-CCT-AGT-CAA-- DNA Template --TCA-AGA-GGA-TCA-GTT-- mRNA CODONS --AUG-UCU-CCU-AGU-CAA-- tRNA ANTICODONS --UAC-AGA-GGA-UCA-GUU-- PROTEIN NORMAL --Met-Ser*-Pro-Ser-Gln-- MUTANT --Met-Cys*-Pro-Ser-Gln-- MUTANT mRNA --AUG-UG*U-CCU-AGU-CAA- MUTANT DNA CODING STRAND --ATG-TG*T-CCT-AGT-CAA-- Sophie, Austria Sophie, Austria DNA Coding strand: --- A T G T C T C C T A G T C A A --- DNA Template strand: --- T A C A G A G G A T C A G T T --- mRNA with codons: --- A U G U C U C C U A G U C A A --- tRNA with anti-codons: --- U A C A G A G G A U C A G U U --- Normal PDSTβ1: --- Met Ser Pro Ser Gln --- Mutant PDSTβ1: --- Met Cys Pro Ser Gln --- Mutant mRNA: --- A U G U G U C C U A G U C A A --- Mutant DNA: --- A T G T G T C C T A G T C A A --- 1. What amino acid does the start codon encode? Methionine. 2. Why don’t all proteins have this amino acid as their first? because of posttranslational modification Daria, Poland Daria, Poland DNA Coding strand: ATG TCT CCT AGT CAA DNA Template strand: TAC AGA GGA TCA GTT mRNA Codons: AUG UCU CCU AGU CAA tRNA Anticodons: UAC AGA GGA UCA GUU Normal PDST: Met Ser Pro Ser Gln Mutant: Met Cys Pro Ser Gln mutant mRNA:AUG UGU CCU AGU CAA Mutant DNA: ATG TGT CCT AGT CAA 1. Met Tina, Austria Tina, Austria Vesna, Slovenia Vesna, Slovenia Anna Italy Anna Italy Working together with my religion colleague, we have arrived at these conclusions. 1. Which amino acid encodes the codon beginning? methionine 2. Why do not all proteins have amino acids as before? N-terminal methionine, although it is the first amino acid, is not present in the N-terminus of all proteins. This is due to a process known as post-translational modification. After a polypeptide has been completely translated by mRNA, it is modified at different points by different enzymes, which are regulated by different factors (internal or external). Maria DC, Italy Maria DC, Italy Being a professor of religion, and collaborating with my science colleague, I was helped to carry out this exercise and to understand that: 1. Which amino acid encodes the start of the codon? methionine 2. Why do not all proteins have amino acids as before? N-terminal methionine, although it is the first amino acid, is not present in the N-terminus of all proteins. This is due to a process known as post-translational modification. After a polypeptide has been completely translated by mRNA, it is modified at different points by different enzymes, which are regulated by different factors (internal or external). Lidia , Ro Lidia , Ro Very interesting... Ayşegül/Turkey Ayşegül/Turkey Mustafa BOZOGLAN, Turkey Mustafa BOZOGLAN, Turkey Gilda/Italy Gilda/Italy DNA Coding strand: ATG TCT CCT AGT CAADNA Template strand: TAC AGA GGA TCA GTTmRNA Codons: AUG UCU CCU AGU CAAtRNA Anticodons: UAC ACA GGA UCA GUUAmino Acid Sequence: Normal PDSTß1: Met Ser Pro Ser Gln Mutant PDSTß1: Met Cys Pro Ser GlnMutant mRna: AUG UGU CCU AGU CAAMutant DNA coding strand ATG TGT CCT AGT CAA1. Met DNA Coding strand: ATG TCT CCT AGT CAADNA Template strand: TAC AGA GGA TCA GTTmRNA Codons: AUG UCU CCU AGU CAAtRNA Anticodons: UAC ACA GGA UCA GUUAmino Acid Sequence: Normal PDSTß1: Met Ser Pro Ser Gln Mutant PDSTß1: Met Cys Pro Ser GlnMutant mRna: AUG UGU CCU AGU CAAMutant DNA coding strand ATG TGT CCT AGT CAA1. Met 📎 Photo Michael, Ireland Michael, Ireland Empty Sonila,Albania Sonila,Albania A little help nedded though Michela, Italy Michela, Italy 📎 Photo Natalia Grushko, Ukraine Natalia Grushko, Ukraine 2) TAC AGA GGA TCA GTT 3) AUG TCU CCU AGU CAA 4) UAC UGA GGA UCA GUU For me it was very interesting, but I have to asked my students about help. in normal in normal Sequence of aminoacids in Sequence of aminoacids in normal sequence: Alice, Italy Alice, Italy Methionin, RNA AUG, DNA TAG, DNA codes many nonsense Methionin, RNA AUG, DNA TAG, DNA codes many nonsense Genetic engineering Genetic engineering is too difficult. I can not do this activity, I'm sorry. Voinea Axinte Costica, Romania Voinea Axinte Costica, Romania 1. Start codon: AUG 2. Not all proteins have the first amino acid amino acid due to the maturation process of the molecules in which the first amino acid is usually removed, depending on the protein structure Ana, Spain Ana, Spain Katarina, Slovenia Katarina, Slovenia 1. (DNA coding) : ..ATG TCT CCT AGT CAA... 2. (DNA template) ...TAC AGA GGA TCA GTT... 3.(mRNA codons) ...AUG UCU CCU AGU CAA... 4. (tRNA anticodons) ...UAC AGA GGA UCA GUU... 5. (Aminoacids sequence- normal) Met-Ser-Pro-Ser-Gln 6.( Aminoacids sequence-mutant) Met-Cys-Pro-Ser-Gln 7.(Mutant mRNA) ...AUG-UGU-CCU-AGU-CAA... 8. (DNA template) ...TAC ACA-GGA-TCA-GTT... 9.. (Mutant DNA coding) ...ATG-TGT-CCT-AGT-CAA... 10. Met 11. Because of post - translation chamodification. Estibaliz, Calgary Estibaliz, Calgary 1)ATG TCT CCT AGT CAA(coding ADN) 2)TAC AGA GGA TCA GTT 3)AUG UCU CCU AGU CAA(mRNA) 4)UAC AGA GGA UCA GUU 5)Sequence of aminoacids in normal sequence: Met-Ser-Pro-Ser-Gln 6)Sequence in mutant: AUGUGUCCUAGUCAA Met-Cys-Pro-Ser_Gln Rossella Lupo, Italy Rossella Lupo, Italy Francesco Piccolo, Italy Francesco Piccolo, Italy This is the screenshot, to comply with the delivery. Francesco Piccolo, Italy Francesco Piccolo, Italy 1. AUG = Met (M); 2. Because it is not enough to have the beginning codon but other sequences are needed that allow the ribosomes to bind to it; among other things, it is not only the AUG (Methionine) codon that initiates the chain but has alternatives (CUG, UUG); finally, Methionine is often removed after the synthesis of some proteins. Nicola, Malta Nicola, Malta 1. Methionine 2. AUG is not the only start codon. Other codons may also initiate translation. Also methionine is not the N terminus for all proteins. Pilar, Spain Pilar, Spain 1. Methionine. 2. Sometimes proteins suffer a maturation post-translational modification, that removes the first amino acid. Angela Italy Angela Italy Discovery Gene therapy is an experimental technique for treating disease by altering the patient's genetic material. Most often, gene therapy works by introducing a healthy copy of a defective gene into the patient's cells. In recent years there have been rapid advancements in techniques that make it easier than ever to edit the human genome. Genome editing techniques, such as CRISPR/Cas9 allow scientists to edit the genome, by removing, replacing, or adding to parts of the DNA sequence. Maria A. - Italy Maria A. - Italy Questions: 1. What amino acid does the start codon encode? Methionine 2. Why don't all proteins have amino acid as their first? The N-terminal methionine, although being the first amino acid, is not present at N-terminus of all proteins. This is because of a process that is known as post-translational modification. After a polypeptide is completely translated from mRNA, it is modified at different places by different enzymes, which are regulated by different (internal or external) factors. Mayte, Spain Mayte, Spain ---TACAGAGGATCAGTT--- ---AUG UCU CCU AGU CAA-- ---UAC AGA GGA UCA GUU-- ---Met-Ser-Pro-Ser-Gln--- ---Met-Ser-Pro-Cys-Gln--- ---AUG UCU CCU UGU CAA-- ---TACAGAGGAACAGTT--- 📎 Document I am so, sorry I am so, sorry. I can’t do it, I don’t know anything about biology. An I don’ t Have time to learn now. Silvia, Italy Silvia, Italy Questions: 1. The AUG start codon encodes for the amino acid Methionine 2. Some proteins are post-processed after encoding, so their starting amino acids are removed Carlo Italy Carlo Italy Marjeta, Slovenia Marjeta, Slovenia 1. Start codon encode Met. 2. Alexandra, Portugal Alexandra, Portugal DNA (Ts) - TACAGAGGATCAGTT mRNA (Codons) - AUGUCUCCUAGUCAA tRNA(anticodons) - UACAGAGGAUCAGUU Aminoacid (1) Met - Ser - Pro - Ser - Gin (2) Met - Ser - Pro - Cys - Gin Mutant mRNA - AUGUCUCCUAGUCAA Mutant DNA - ATGTCTCCTAGTCAA 📎 Photo Marina del Barco SPAIN Marina del Barco SPAIN CRISPR ACTIVITY Questions: 1. The AUG start codon is always Methionine (Met). 2.Start codon could be removed after protein synthesis. Activity: DNA coding strand ATG TCT CCT AGT CAA DNA template strand TAC AGA GGA TCA GTT mRNA codons AUG UCU CCU AGU CAA tRNA anticodons UAC AGA GGA UCA GUU Amino acid sequence: Normal PDSTB1 Met-Ser-Pro-Ser-Gln Mutant PDSTB1 Met-Cys-Pro-Ser-Gln Mutant mRNA AUG UGU CCU AGU CAA Mutant DNA coding strand ATG TGT CCT AGT CAA Metionine Metionine Cause some proteins undergo a post-translation process in which this first aminoacid can be eliminated *ATG TCT CCT AGT CAA(coding ADN) *TAC AGA GGA TCA GTT *AUG UCU CCU AGU CAA(mRNA) *UAC AGA GGA UCA GUU(anticodon) *Sequence of aminoacids in normal sequence: Met-Ser-Pro-Ser-Gln *Sequence in mutant: AUGUGUCCUAGUCAA Met-Cys-Pro-Ser_Gln Cecilia Fontanella, Italy Cecilia Fontanella, Italy My answers are reported directly on the image below Lucia Italy Lucia Italy 1) Met 2) Met in the others sequences is remove Marzia, Italy Marzia, Italy 1- Met 2- Met can be cut after sinthesys Ana Lereno, Portugal Ana Lereno, Portugal 1. Met 2. Met is only need to begin the amino acid sequence. Many proteins don't have this amino acid in the beginner of the sequence and for that, is remove.

Mirela, Romania

mirelaemiliapop — 2018-04-15 17:25:26 UTC

Beatriz Sanchez, Spain

beatriz_sangar — 2018-04-15 18:27:07 UTC

DNA Coding strand ATGTCTCCTAGTCAA
Acording with the video
A--T G--C
DNA template strand
TACAGAGGATCAGTT
but I don´t know anything about DNA I am a primary teacher and the activity was interesting but too difficult

Sarah, USA

sab205 — 2018-04-15 23:16:50 UTC

I am with Beatriz, the other primary teachers, and all those whose heads this is waaaaaaaaaaaaaay over! It is interesting, and it was fun to puzzle with it for a little bit, but the amount of time (and tutoring) it would take for me to complete (let alone understand) this activity does not seem worth the investment of time for me (since this will not be helpful knowledge for my classroom or any other life situation that I can think of being involved in). Sorry! KUDOS to all of you who get it! :)

Beatriz Sánchez, Spain

beatriz_sangar — 2018-04-17 18:15:55 UTC

After a friend with moe knowledge helped me I was ale to figure it out.

DNA Template Strand:
TACAGAGGATCAGTT

mRNA Codons:
AUG;UCU;CCU;AGU;CAA

tRNA Anti codons;
UAC;AGA;GGA;UCA;GUU

Amino Acid Sequence:
Met Ser Pro Ser Gin

Mutated Amino Acid:
Met Cys Pro Ser Gin

Mutated mRNA:
AUG;UGU;CCU;AGU;CAA

Mutant DNA Coding Strand:
ATGTGTCCTAGTCAA

1) Met 2)IT is removed after protien synthesis

Ozrenka, Croatia

2018-04-17 19:46:29 UTC

Áine, Ireland

ainemc — 2018-04-18 11:07:01 UTC

Samet Yavuz TERZİ , TURKEY

2018-04-19 10:52:32 UTC

This activity on the DNA spiral was adopted by the students and provided fun learning.

Maria S., Italy

2018-04-21 14:59:46 UTC

My Activity on the DNA:
1. TACAGAGGATCAGTT
2. AUG UCU CCU AGU CA A
3. UAC AGA GGA UCA GUU
4. S P S Q
5. C P S Q
6. AUG UGU CCU AGU CAA
7. AT GTGTCCTAGTCAA

QUESTIONS
1. MET

Ourania Lampou, Greece

2018-04-21 16:47:47 UTC

1 Methionine
2. Because it has been removed after synthesis

Salima, India

2018-04-21 17:22:13 UTC

DNA template strand
TAC AGA GGA TCA GTT
mRNA codon
AUG UCU CCU AGU CAA
tRNA codon
UTC AGA GGA UCA GUU
Amino acid sequence
Normal
Met Ser Pro Ser Gln
Mutant
Met Cys Pro Cys Gln
Mutant mRNA
AUG UGU CCU UGU CAA
Mutant DNA coding strand
ATG TGT CCT AGT CAA

Answers
1. Methionine
2. Probably after the protein is made it is removed from the sequence

EUGENIA ROMANIA

2018-04-23 19:11:36 UTC

1. DNA C - ATGTCTCCTATGTCAA

2. DNA T –TACAGAGGATCAGTT

3. m RNA –AUG UCU CCU AGU CAA

4. t RNA -uac aga gga ucc guu

5. normal met- ser-pro-ser-gin

6. mutant met-cys-pro-ser-gin

7. mutant Mrna AUG UGA CCU AGU CCA

8. mutant DNA ATG TGT CCT AGT CAA

nazım kırdaş

2018-04-24 09:34:12 UTC

The use of DNA code was an enjoyable and challenging activity. we had difficulty reaching the result. but we reached the final result.

mehtap öztürk

2018-04-24 23:13:18 UTC

^{DNAC/ATGTCTCCTATGTCAA}

^{DNAT/TACAGAGGATCAGTT}

^{mRNA/AUGUCUCCUAGUCAA}

^{tRNA/UACAGAGGAUCCGUU}

^{normal met- ser-pro-ser-gin}

^{mutant met-cys-pro-ser-gin}

^{mutant mRNA/AUG UGA CCU AGU CCA}

^{mutant DNA/ATG TGT CCT AGT CAA}

Mehtap ÖZTÜRK

2018-04-24 23:19:19 UTC

^{DNA C ( ATGTCTCCTATGTCAA)}

^{DNA T (TACAGAGGATCAGTT)}

^{m RNA (AUGUCUCCUAGUCAA)}

^{t RNA (UACAGAGGAUCCGUUU)}

^{normal met- ser-pro-ser-gin}

^{mutant met-cys-pro-ser-gin}

^{mutant mRNA (AUGUGACCUAGUCCA)}

^{mutant DNA (ATGTGTCCTAGTCAA)}

Turkey

2018-05-10 20:33:10 UTC

1. DNA C - ATGTCTCCTATGTCAA

2. DNA T –TACAGAGGATCAGTT

3. m RNA –AUG UCU CCU AGU CAA

4. t RNA -uac aga gga ucc guu

5. normal met- ser-pro-ser-gin

6. mutant met-cys-pro-ser-gin

7. mutant Mrna AUG UGA CCU AGU CCA

8. mutant DNA ATG TGT CCT AGT CAA

Turkey/Meryem

megri_1971 — 2018-05-19 21:06:28 UTC

It seems so fun to solve. But my students way too little to solve this. I'm gonna show this graphic to them. But like I said it will be difficult for them to solve.

Hülya Kartal

2018-12-18 20:56:58 UTC

DNA

çağdaş turan

113aytoldu — 2019-03-21 08:29:54 UTC

This activity on the DNA spiral was adopted by the students and provided fun learning.

Δημήτρης

2019-06-16 08:44:40 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA

DNA Template strand:
TAC AGA GGA TCA GTT

mRNA Codons
UAC AGA GGA UCA GUU

tRNA anticodons
AUG UCU CCU AGU CAA

Amino acid sequence

Normal M S P S Q

Mutant M S P C Q

work with the second Serine

Mutant mRNA
UAC AGA GGA ACA GUU

Mutant DNA Coding strand:
ATG TCT CCT TGT CAA

Questions

1. Methionine

2. post translation modification

Ivica G.

ivica_gregurec — 2019-09-09 20:36:23 UTC

DNA
ATG TCTCCTTGTCAA