The Agar Diffusion Assay — Its Quantitative Basis by DR. DEHIN BHAGAT https://padlet.com/dehinbhagat/zm3hp8uslqchiye4 EASY UNDERSTANDING en-us 2020-07-30 04:49:11 UTC 2026-02-04 22:24:34 UTC hello@padlet.com https://padlet.net/icons/png/2705.png THE THEORY OF ZONE FORMATION dehinbhagat https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665604060
  • Interaction b/w drug vs culture
  • Physical, chemical and biological nature
  • Agarose: long chains of galactose units with alpha and beta linkages
  • 96% water
  • Provide pore with no interaction
  • Easy for diffusion
  • 3 or 4 mm thick gel
  • Sharper zone boundaries and critical time
  • Agar and ion exchange activity
  • Narrow tube: Both theoretical and practical grounds that the
    square of the width of the inhibition zone was directly proportional to the logarithm
    of the concentration of streptomycin.


    • ]]>     2020-07-30 04:51:17 UTC https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665604060     Cooper and Gillespie: dehinbhagat https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665608467 Studies consider the mathematics of diffusion of the antibiotic through the agar gel, growth pattern of the test organism, and interaction of the antibiotic with the living cell.



    ]]>     2020-07-30 04:58:14 UTC https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665608467     Terminology dehinbhagat https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665616908 2020-07-30 05:12:20 UTC https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665616908 Mathemetical Model dehinbhagat https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665619522 2020-07-30 05:17:10 UTC https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665619522 HISTORICAL INTRODUCTION dehinbhagat https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665619978
  • Penicillin was discovered by Alexander Fleming in 1929.
  • Howard Florey and Ernst Chain
  • Norman George Heatley, a biochemist, was assigned the task of developing conditions of growth of the penicillin-producing fungus so as to obtain enough active substance for realistic investigation of its medical potential.
    • ]]>     2020-07-30 05:18:02 UTC https://padlet.com/dehinbhagat/zm3hp8uslqchiye4/wish/665619978