DNA Technology by Djakata Walker https://padlet.com/djawal2827/zizwzura07t1 en-us 2016-11-09 17:26:14 UTC 2023-03-06 23:45:06 UTC hello@padlet.com What is a Gel Electrphoresis? djawal2827 https://padlet.com/djawal2827/zizwzura07t1/wish/136488083 -  Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size.]]> 2016-11-09 17:28:35 UTC https://padlet.com/djawal2827/zizwzura07t1/wish/136488083 Why do scientist use a Gel Electrophoresis? djawal2827 https://padlet.com/djawal2827/zizwzura07t1/wish/136488545 - Scientists use gel electrophoresis whenever they need to sort DNA strands according to lengths. This technique is also useful for separating other types of molecules, like proteins.]]> 2016-11-09 17:29:45 UTC https://padlet.com/djawal2827/zizwzura07t1/wish/136488545 Explain the steps scientist use to perform a Gel Electrophoresis experiment. djawal2827 https://padlet.com/djawal2827/zizwzura07t1/wish/136489718 - The first step to gel electrophoresis is to set the gel matrix. Agarose is used to separate DNA molecules, and acrylamide is used to separate proteins. The gel starts off as a liquid, which is poured into a molding tray. A comb is placed in the liquid matrix so that when the matrix solidifies, wells are formed to load samples in them. Once the gel has solidified, it is removed from the mold and placed in a special apparatus where current can be applied. A buffer that can act as a conductor of electricity is poured around the matrix.

The samples of bio molecules are usually mixed with a substance of high density (a viscous dye) so that they sink to the bottom of the well instead of floating away in the buffer. The dye also helps track the progress of the experiment. Each sample is loaded in a separate well. One of the wells is usually assigned for loading a marker, which has a set of fragments whose sizes are already known in order to allow for comparison with the samples being loaded.

When the current is switched on, the samples tend to move towards the positively charged side of the apparatus since the phosphate backbones of the molecules confer a negative charge on them. After the samples have run a sufficient distance, the matrix is studied to view the bands that are formed by the separation of the molecules.
]]> 2016-11-09 17:32:54 UTC https://padlet.com/djawal2827/zizwzura07t1/wish/136489718 djawal2827 https://padlet.com/djawal2827/zizwzura07t1/wish/136491065 2016-11-09 17:36:02 UTC https://padlet.com/djawal2827/zizwzura07t1/wish/136491065