Special Topics in Biotechnology by Luigi De Girolamo https://padlet.com/ginodegirolamo/zgjgnbytsqv69696 en-us 2024-02-12 12:11:17 UTC 2024-02-12 17:31:02 UTC hello@padlet.com Introduction ginodegirolamo https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880878591 2024-02-12 12:11:55 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880878591 Methods ginodegirolamo https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880878864 2024-02-12 12:12:20 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880878864 Figures ginodegirolamo https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880879518 2024-02-12 12:13:05 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880879518 Tables ginodegirolamo https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880879705 2024-02-12 12:13:24 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2880879705 Plant genotype https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242009 2024-02-12 17:12:01 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242009 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242058 Examples

]]> 2024-02-12 17:12:03 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242058 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242360 Plant used

Gene to be cloned

Methods used

Vector construction


]]> 2024-02-12 17:12:18 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242360 Types https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242391 2024-02-12 17:12:20 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881242391 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243318 Recombinant technology of plants

]]> 2024-02-12 17:13:02 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243318 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243469 Genetic engineering of plant antibodies

]]> 2024-02-12 17:13:10 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243469 Definition of terms and thereafter linking all terms together https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243604 2024-02-12 17:13:18 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243604 Introduction https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243614 The aim


]]> 2024-02-12 17:13:18 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243614 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243781 Background information


]]> 2024-02-12 17:13:27 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243781 Introduction https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243949 Literature review which shows why using plant is better. The mechanisms which people already use. Aims of our review.


]]> 2024-02-12 17:13:34 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881243949 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244064 Discovery

]]> 2024-02-12 17:13:39 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244064 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244245 Plants as production host for mAbs

]]> 2024-02-12 17:13:49 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244245 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244252 Aim,plant used, Tecniques used, abstract

]]> 2024-02-12 17:13:49 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244252 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244707 Introduction of vectors, antibody production, plant engineering

]]> 2024-02-12 17:14:02 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244707 Background on previous plant use as antibody production https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244740 2024-02-12 17:14:03 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244740 Explain aim of the topic https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244790 2024-02-12 17:14:05 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244790 Types sub types https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244855 2024-02-12 17:14:08 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244855 Recombinant technology https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244946 The use of plant in Biotechnology

Plant Antibodies

]]> 2024-02-12 17:14:13 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881244946 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245150 Aim

]]> 2024-02-12 17:14:22 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245150 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245288 Aim

]]> 2024-02-12 17:14:29 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245288 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245754 Plant antibodies

]]> 2024-02-12 17:14:52 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245754 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245832 Purpose/ objective

]]> 2024-02-12 17:14:56 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245832 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245872 how plant-based anyibody and vaccine production can contribute to global health

]]> 2024-02-12 17:14:58 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881245872 Benefits and the restrictions https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881246428 2024-02-12 17:15:27 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881246428 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881246797 Road map how will essay answer the question

]]> 2024-02-12 17:15:44 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881246797 Aim for production of antibodies from plants (Why)? https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881246871 2024-02-12 17:15:48 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881246871 Challenges faced https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247033 2024-02-12 17:15:57 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247033 Purpose/ objective https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247122 2024-02-12 17:16:01 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247122 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247390 Examples of successful casestudies on plant based antibodies

]]> 2024-02-12 17:16:14 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247390 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247569 genetic engennering

]]> 2024-02-12 17:16:24 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247569 Recombinant DNA technology and the major challenges involved https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247593 2024-02-12 17:16:25 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881247593 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881248156 Analysis

]]> 2024-02-12 17:16:51 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881248156 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249040 History of antibodies in plants

Target sites for antibody action



]]> 2024-02-12 17:17:40 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249040 Definition https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249049 2024-02-12 17:17:40 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249049 Examples of plant based recombinant proteins in clinical trials https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249220 2024-02-12 17:17:49 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249220 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249283 Plants could be an abundant sources of recombinant proteins.

]]> 2024-02-12 17:17:52 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881249283 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881260394 plant species selected for the study

]]> 2024-02-12 17:27:42 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881260394 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881260812 Data analysis

]]> 2024-02-12 17:28:00 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881260812 Materials required https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881260979 2024-02-12 17:28:08 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881260979 Transfection https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261467 Translation

Gene expression

]]> 2024-02-12 17:28:31 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261467 Mention Techniques performed https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261683 2024-02-12 17:28:42 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261683 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261719 plant transformation, purification of protein

]]> 2024-02-12 17:28:45 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261719 Techniques used or can be used https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261798 2024-02-12 17:28:50 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881261798 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262018 PCR and DNA fingerprinting

]]> 2024-02-12 17:29:02 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262018 Test https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262053 Test

]]> 2024-02-12 17:29:05 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262053 Translation https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262076 The DNA and RNA purification

]]> 2024-02-12 17:29:06 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262076 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262139 Extraction

]]> 2024-02-12 17:29:09 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262139 Technique https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262222 2024-02-12 17:29:13 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262222 Methods https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262606 Dive deep in the details of exact processes by which antibodies can be made from plants. This would start from obtaining gene of interest to purifying antibodies and preparing them for human use.

]]> 2024-02-12 17:29:31 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262606 Tools https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262621 2024-02-12 17:29:32 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262621 DNA and RNA purification https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262660 2024-02-12 17:29:34 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262660 Vector selection, amplification of gene of interest using PCR, expression system and Cloning strategies, purification of the recombinant protein. https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262750 2024-02-12 17:29:38 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881262750 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263196 Why we are using the method,how it can give reliable results

]]> 2024-02-12 17:30:01 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263196 Genetic engineering and recombinant biotechnology. https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263533 2024-02-12 17:30:20 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263533 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263753 Procedure and material used in production of recombinant Abs in plant.

Why the method is used.

Brief role of any chemicals if used.



]]> 2024-02-12 17:30:33 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263753 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263829 Explain why and how that particular method is suitable for our study.

]]> 2024-02-12 17:30:38 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881263829 Experiments https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264242 2024-02-12 17:31:02 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264242 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264468 extract gene of interest

amplification

dna purification

transfection or transduction


]]> 2024-02-12 17:31:11 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264468 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264505

]]> 2024-02-12 17:31:13 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264505 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264571 selecting plant species

using recombinent DNA technology for production of vaccines



]]> 2024-02-12 17:31:16 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264571 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264608 Any precautions

]]> 2024-02-12 17:31:18 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264608 https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264732 Cloning and expression

]]> 2024-02-12 17:31:26 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881264732 Technique https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881265105 2024-02-12 17:31:45 UTC https://padlet.com/ginodegirolamo/zgjgnbytsqv69696/wish/2881265105