Photosynthesis Lab by Donesha Robinson https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5 Exploring the world of plants and their pigments while balancing a lot of test tubes on books en-us 2020-07-05 17:13:58 UTC 2026-02-14 23:14:27 UTC hello@padlet.com https://padlet.net/icons/png/1f966.png Purpose ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647823538 2020-07-05 20:53:05 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647823538 The Use of Sodium Bicarbonate ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647823641 When mixed with water, sodium bicarbonate produces carbonic acid which eventually gives off carbon dioxide. This along with light provides an ideal environment for the plant leaves to photosynthesis and we can measure their rate by seeing how they rise to the surface of the sodium bicarbonate-water mixture. ]]> 2020-07-05 20:53:28 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647823641 Exposing the leaves to household light ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647824986 The creative solutions of a home biology lab ]]> 2020-07-05 20:57:52 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647824986 Results of the Positive Control ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647826217 All of the leaves did not float to the surface. I think maybe the solution could have benefited from more sodium bicarbonate, but after 15 minutes, 5 leaves did rise to the surface ]]> 2020-07-05 21:00:37 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647826217 Soapy Water ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647827366 Soapy water does not provide an ideal environment for the leaves to photosynthesis. We should not expect the leaves to rise, and for the most part they didn't. Two leaves did manage to make their way to the surface]]> 2020-07-05 21:03:42 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647827366 The experiment was repeated in different environments ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647828366 To test how differing environments affect photosynthesis, I placed a CO2 cup into the fridge for 15 minutes and a soapy cup outside for 15 minutes. Not surprisingly, the cup in the fridge did not see any leaves rise to the surface. The soapy cup placed outside in the hot humidity, saw another 1 additional leaf rise to the surface (one leaf never fell). ]]> 2020-07-05 21:06:59 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647828366 The Outside Soapy Water ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647867034 Due to some foreseen laziness on my part, I left the soapy water outside with the leaves for about 3 hours after the experiment and to my surprise, all the leaves came to the surface. I don't know whether it was because of the soap or if the experiment just needed more time. In the future, this experiment may benefit from extra time and more sodium bicarb in the water of the positive control ]]> 2020-07-05 23:00:54 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647867034 Making the Leaf Discs ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647869294 2020-07-05 23:05:45 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647869294 In this experiment, we explore the effect of light on the chloroplasts isolated from the leaf ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647930045 During the light dependent reactions of photosynthesis, there is a release of protons. To see if we can create this reaction, we use a DPIP solution that changes colors from blue to clear in the presence of protons. We isolate the chloroplasts from spinach leaves, introduce to them to a sucrose solution, and add a phosphate buffer and DPIP solution. We put them near a light source and wait to see what happens. ]]> 2020-07-06 01:29:23 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647930045 Test tube 1 contains the DPIP solution without the chloroplasts. Test tube 2 contains the chloroplasts with the DPIP solution and will stay in the dark. Test tube 3 contains the chloroplasts and the DPIP solution and will be exposed to light. (Note: It took me a few minutes to realize that test tube 2 needed to be completely wrapped in foil so it could be shielded from light. Don't laugh) ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647951673 2020-07-06 02:11:51 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647951673 ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647955536 2020-07-06 02:18:58 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647955536 After sitting near a lamp for several hours and not seeing any changes, I hypothesized that the light source wasn't nearly strong enough and had to find a slightly precarious solution, but it worked out ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647955763 2020-07-06 02:19:23 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647955763 The New Setup Much Closer to a Light Source ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647957470 (Note: After the picture was taken, the cup was rotated so test tube 3 and test 2 tube were both facing the light bulb) ]]> 2020-07-06 02:22:46 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647957470 Letting the Spinach Sit ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647958787 2020-07-06 02:25:32 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647958787 Isolating the Chloroplasts ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647959228 2020-07-06 02:26:29 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647959228 Test 1 tube exposed to light, did not change from its original blue color. Test tube 2, that stayed wrapped in the foil, did not change color much. Test tube 3 became much lighter than test tube 2, indicating that the chloroplasts in the lights produced enough protons from the light dependent reaction to change the color of the DPIP solution in the test test ddrobi https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647980016 2020-07-06 03:06:48 UTC https://padlet.com/ddrobi/zd7dzf0qd4iw7ro5/wish/647980016