Enzyme Lab by Malaysia Wimberly https://padlet.com/tallamal59/yc20zh73cli1 http://www.schooltube.com/video/0f9b17a9d14e4b93af83/Liver-Enzyme-Lab en-us 2017-02-14 19:00:57 UTC 2017-02-16 05:09:17 UTC hello@padlet.com Purpose/Problem tallamal59 https://padlet.com/tallamal59/yc20zh73cli1/wish/153848592  This lab teaches us how enzymes in the liver react with the peroxide and also teach us that an increase in temperature will stop the enzymes from functioning correctly 
Background information
  • The peroxide produced by our cells is poisonous
  • Our body create catalysts to break down the poisonous peroxide

]]> 2017-02-14 19:34:51 UTC https://padlet.com/tallamal59/yc20zh73cli1/wish/153848592 Introduction tallamal59 https://padlet.com/tallamal59/yc20zh73cli1/wish/153848747 Enzymes break down substrates that would pass undigested by catalyzing the breakdown of these substrates.]]> 2017-02-14 19:35:21 UTC https://padlet.com/tallamal59/yc20zh73cli1/wish/153848747 Material tallamal59 https://padlet.com/tallamal59/yc20zh73cli1/wish/153849048
  • 5 Test Tubes
  • Matches
  • Peroxide
  • Water/ Boling water
  • Eyedropper
  • Liver
  • Wooden Splints
  • Lighter 
    • ]]>     2017-02-14 19:36:17 UTC https://padlet.com/tallamal59/yc20zh73cli1/wish/153849048     Data/Results tallamal59 https://padlet.com/tallamal59/yc20zh73cli1/wish/153850380 Test tube #1 Water and Liver  | Test tube #1 had no reaction 
    Test tube #2 Peroxide and Liver  | The reaction of test tube #2 was bubbling 
    Test tube #3 Peroxide and wooden splint | Test tube #3 also had no reaction 
    Test tube #4  Peroxide, Liver and wooden splint | Test tube #4 started to bubbles. When the wooden splint was added to the bubbles 
    Test tube #5 Boiled Liver, Peroxide and water  | Test tube #5 had no reaction 


    • In test 1 when the liver was added to the water no reaction occurred 
    • In test 2 when the liver was added to the peroxide it created bubbles 
    • In test 3 when the wooden splint was put into the peroxide the flame was put out. From this, I learned that peroxide is not flammable and it also doesn't contain oxygen
    • In test four when the wooden splints fire was placed above the bubbles a spark appeared. From this, I learned that the bubbles are made out of water and oxygen 
    • In test 5 when the boiled liver was added a different reaction occur than the last test because the enzymes within the liver became denatured when the temperature increased
    ]]>     2017-02-14 19:39:56 UTC https://padlet.com/tallamal59/yc20zh73cli1/wish/153850380     Discussion tallamal59 https://padlet.com/tallamal59/yc20zh73cli1/wish/153851641 1. What are enzymes?  What are their roles in chemical reactions?
    Enzymes are organic catalysts. In a chemical reaction, they are used to speed up the rate of that function.

    2.  Describe what happened to test tube 2 when hydrogen peroxide was added to the liver. What gas was produced?  What liquid was left behind?
    When the liver was added to the peroxide bubbles started to form. Water and oxygen were produced 

    3.  What effect does the red hot wood have on test tube 4? WHY is the flame being produced? 
    When the flame of the wooden splint touched the bubbles a spark was created. The spark was created because the liver enzymes and peroxide produced pure oxygen 

    4.  What effect does temperature have on the enzymes (Test tube 5)?
    When the temperature increases higher than its optimal temperature the enzymes becomes denature making them unable to do their functions. 
    ]]>     2017-02-14 19:43:47 UTC https://padlet.com/tallamal59/yc20zh73cli1/wish/153851641     Conclusion tallamal59 https://padlet.com/tallamal59/yc20zh73cli1/wish/153852517 In this lab I learned In this lab I learned enzymes are can  break down substrates but can only function correctly in the correct temperature. An example of this is in test tube 5 when the wooden splint was added to the boiling water, liver, and peroxide but nothing happened because the temperature increased higher than its optimal temperature the enzymes became denatured]]> 2017-02-14 19:46:26 UTC https://padlet.com/tallamal59/yc20zh73cli1/wish/153852517 Procedure tallamal59 https://padlet.com/tallamal59/yc20zh73cli1/wish/153866220 Test One/Water and liver
    Step 1. In test tube 1 pour water up to the 2 cm mark 
    Step 2. Add a piece of liver 

    Test Two/Peroxide Liver 
    Step 1. In test tube 2 pour peroxide to the 2 cm mark.
    Step 2. Next, add a piece of liver 

    Test Three/ Peroxide and wooden splint
    Step 1.  In test tube 3 pour peroxide to the 2 cm mark.
    Step 2. Light one of the wooden splints and wait 6 seconds before blowing it out. 
    Step 3. Put hot end of the wooden splint into the peroxide

    Test Four/Peroxide, Liver, and wooden splint
    Step 1. In test tube 4 pour peroxide to the 2 cm mark
    Step 2. Add liver to the peroxide
    Step 3. Light one of the wooden splints and wait 6 seconds before blowing it out.
    Step 4.  Put hot end of the wooden splint by the bubbles 

    Test Five/Boiled water, Peroxide and liver
    Step 1. In test tube 5 pour 2 cm of boiling water and a piece of liver and let boil for a while 
    Step 2. Next, pour peroxide to the 2 cm mark


    ]]>     2017-02-14 20:40:34 UTC https://padlet.com/tallamal59/yc20zh73cli1/wish/153866220