SMBT3613 Research Methodology by Mohd Helmi Sani

SMBT3613 Research Methodology by Mohd Helmi Sani https://padlet.com/helmisani/researchproblem Formulation of Research Problem en-us 2018-05-03 02:14:26 UTC 2021-05-08 07:03:27 UTC hello@padlet.com https://padlet.net/icons/png/1f4a1.png Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold helmisani https://padlet.com/helmisani/researchproblem/wish/1381937736 https://www.nature.com/articles/s41598-021-85149-x.pdf

]]> 2021-04-05 03:30:07 UTC https://padlet.com/helmisani/researchproblem/wish/1381937736 Current Status and Potential Applications of Underexplored Prokaryotes helmisani https://padlet.com/helmisani/researchproblem/wish/1381946389 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970974/pdf/41598_2021_Article_85149.pdf

]]> 2021-04-05 03:35:21 UTC https://padlet.com/helmisani/researchproblem/wish/1381946389 Group Discussion on Research Problem https://padlet.com/helmisani/researchproblem/wish/1396750371 Group Members :
Norarina Suraya (A18SC0431), Norazrin Anis (A18SC0432),
Nur Aisyah (A18SC0437),
Nor Azlia Aziera (A18SC0428),
Nur Anis Nadia (A18SC0440)

Article 1: Current Status and Potential Applications of Underexplored Prokaryotes

Discovery of underexplored culturable and unculturable prokaryotes which may clarify their unspoken novelties and function has always been abandoned.
These rare prokaryotes can be unculturable microorganisms since researchers found that it is hard to cultivate the prokaryotes under laboratory condition.
The rare prokaryotes are still under studied and explored because of the limitations in their discovery and application.
This is because there is insufficient information about underexplored prokaryotes and hard-to-cultivate microorganisms that are actually important to link in terms of biodiversity and their biological roles in the environment.
Several recommendations for cultivating unexplored prokaryotes include paying attention to isolation and growth preparation, reconsidering sampling sites, and changing cultivation methods.

Article 2: Epidermal and Fibroblast Growth Factors Incorporated Polyvinyl Alcohol Electrospun Nanofibers as Biological Dressing Scaffold

There are many limitations in conventional wound dressing and it opens new study and research of skin regeneration and reconstruction by using biological scaffold as a tool to improve in every aspect such as healing time, process of treatment and effectiveness.
Some conventional wound dressing do not allow the typical physiological environment to facilitate the wound healing process due to their insufficiency in biological activities and response which can be addressed by scaffold utilization.
Absorption/immobilization, coaxial/multiaxial and emulsion techniques are complicated because of their material selection, solvent combination and specific volatility while the emulsion technique requires specific properties of molecules, polymers and solvent combinations.
Since PVA’s solubility limited its application in the pharmaceutical field, especially for the partially-hydrolysed PVA, it is crosslinked before utilization to reduce its hydrophilicity. However, crosslinked PVA nanofiber can still expose the tissue to toxicity risks.

]]> 2021-04-08 16:42:05 UTC https://padlet.com/helmisani/researchproblem/wish/1396750371 Group Discussion on Research Problem https://padlet.com/helmisani/researchproblem/wish/1399135164
Group Members:

YEOH SHIR LING A18SC0495
LOIS LO TZU HUI A18SC0408
TAN ZEN DONG A18SC0487
PUA LEI WEN A18SC0467
TAN JIA YONG A18SC0485

Journal_Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold

Free association approach

Problem with skin and reconstruction
High medical treatment cost problem
Problem with not unique scaffold properties
Fabrication of scaffold technique problem
Epithelialization and proliferation of dermal fibroblast

Small conclusion:
The study of epidermal and fibroblast growth factors to improve Electrospinning nanofibers technique in solving demanding high cost medical treatment issues towards skin regeneration and reconstruction.

Journal_Current Status and Potential Applications of Underexplored Prokaryotes

Free association approach

Time-consuming in exploring rare prokaryotes
High research cost
Requiring new advance biotechnology to look for potential microbe

Small conclusion:
The Discovery of rare prokaryotes and its potential functions and applications for future use are less. Therefore, problems and suggestions in culturing the underexplored prokaryotes need to be taken more actions.

]]> 2021-04-09 08:50:23 UTC https://padlet.com/helmisani/researchproblem/wish/1399135164 https://padlet.com/helmisani/researchproblem/wish/1399859771 MASHITOH BINTI AHMAD (A18SC0409)

There are many unpopular prokaryotes and microorganisms still remain under-explored and usually the researchers neglected it .Apart from that, there are many challenging / obstacles due to the lack of information related to the microorganisms or prokarryotes. ]]> 2021-04-09 13:35:18 UTC https://padlet.com/helmisani/researchproblem/wish/1399859771 Group Discussion on Research Problem iqashfi https://padlet.com/helmisani/researchproblem/wish/1400080560 Groupmate’s name:

1) AZRIE FAUZY B ABDULLAH

2) MUHAMMAD SUHAIL B MOHD ARIFFIN

3) IMRAN QASHFI B ISMAIL

4) MOHAMMAD AL-FAYYADH B MD NOOR

5) NOR TAIB ARASH B YUSOFF

Article 1: Current Status and Potential Applications of Underexplored Prokaryotes

Massive quantities of experimental findings and knowledge generated from the well-known prokaryotic strains. Therefore, this study provides an update for uncommon and unculturable prokaryotes, compiling various research efforts.
Researchers are required to comply with most, if not all requirements of Minimum Information about a Single Amplified Genome (MISAG) before submitting SAG sequences to databases.
It is less understood that certain underexplored prokaryotes also have biological roles in the environment, in particular in geochemical cycles of carbon, nitrogen, and sulfur.

Article 2: Epidermal and Fibroblast Growth Factors Incorporated Polyvinyl Alcohol Electrospun Nanofibers as Biological Dressing Scaffold

Several conventional wound dressings do not allow the typical physiological environment to facilitate the wound healing process due to lack of biological activities and responses
Both absorption/immobilization and coaxial/multiaxial techniques are complicated and require particular attention to material selection, solvent combination and specific volatility
the nanofiber the crosslinked PVA nanofiber still exposing the tissue to additional toxicity risks
The variations found to reflect the capability of the utilized PVA in this study to carry the proteins to the burned tissue within an acceptable period can not confirm the design influence as the difference exists but can not differentiate the most influencing design.

]]> 2021-04-09 14:22:24 UTC https://padlet.com/helmisani/researchproblem/wish/1400080560 Group discussion on research problem hnnhndiah https://padlet.com/helmisani/researchproblem/wish/1400674747 Group member :
Hannah Nadiah binti Zulkefli
(A18SC0393)
Nurasyura Najla binti Basri (A18SC0456)
Fatin Nabilah binti Rakib (A18SC0391)
A'dlina binti husny @ Badrul Hisham
(A18SC0373)

Article 1 : Current Status and Potential Application of Underexplored Prokaryotes
- Researchers have been paying less attention to uncommon prokaryotes and hard-to-cultivate microorganisms
- By exploring these prokaryotes; their novelties, function and application in industry can be discovered
- Suggestions on how to isolate or cultivate underexplored prokaryotes : modify method of isolation and growth preparation , reconsider the sampling sites (location with minimum human activities) and modify cultivation strategy (in situ and ex situ cultivation)
-DNA sequencing strategies like MAG, SAG and WGS enable researchers to discover the genomic contents of underexplored prokaryotes - MAG is the most effective way
- The type and preparation of samples have a significant impact on the quality of MAGs and it needs computational ability to process large amounts of sequencing read data and perform downstream analysis.
- Limitation functional-based metagenomics is expensive and is not affordable on a larger scale

Article 2 : Epidermal and Fibroblast Growth Factors Incorporated Polyvinyl Alcohol Electrospun Nanofibers as Biological Dressing Scaffold
-Skin regeneration and reconstruction are currently a demand healthcare area due to the global population growth. The skin’s external and internal inhibitory factors can delay wound healing and may trigger a chronic wound construction that will increase medical treatment cost.
-There are various techniques have been utilized to fabricate scaffolds such as solvent casting, freeze-drying, phase separation, gas foaming, electrospinning, fibre bonding, melt moulding, prototyping, powder compaction, supercritical fluid and fibre mesh.
-However, Electrospinning is an inexpensive and appropriate way to fabricate nanofiber meshes.
-There are several method to obtain the result, for example, characterization analyses, wettability analysis, tensile analysis and Cell culture of human dermal fibroblasts (HDFs)]]> 2021-04-09 16:34:49 UTC https://padlet.com/helmisani/researchproblem/wish/1400674747 Group Discussion on Research Problem aniszaid99 https://padlet.com/helmisani/researchproblem/wish/1401977586 Group Members :
Wan Nooranis bt Wan Zaid (A18SC0491)
Nur Isyafatira bt Japri (A18SC0446)
Nur Amilia bt Ahmad Sukri (A18SC0439)
Nurul Shazleen Natasha bt Mohd Miskam (A18SC0465)

Article 1: Current Status and Potential Applications of Underexplored Prokaryotes

- Uncommon prokaryotes and hard-to-cultivate microorganisms were still under discovery which led to inadequate fundamental knowledge and has limited application.
- Researchers neglected the uncommon prokaryotes and hard-to-cultivate microorganisms.
- There are some methods to cultivate the underexplored prokaryotes such as focusing on isolation and growth preparation, reconsider sampling sites and modifying cultivation methods
- The exploration of underexplored prokaryotes should be done more to enhance their application in industry or human health.
-Some rare prokaryotes might offer great advantages to the industrial application but the obstacle to cultivate or to exploring the rare prokaryote might give the limitation to the researcher to work on it.

Article 2: Epidermal and Fibroblast Growth Factors Incorporated Polyvinyl Alcohol Electrospun Nanofibers as Biological Dressing Scaffold
- Several conventional wound dressings do not allow the typical physiological environment to facilitate the wound healing process due to lack of biological activities and responses.
- Electrospinning techniques which are absorption or immobilization and coaxial or multiaxial techniques are complicated and require particular attention to material selection, solvent combination and specific volatility.
-The wrong technique chosen to fabricate scaffold will affect the efficacy scaffold properties where the fiber will lack of smoothness and there will be present of bead in the electrospun fibers.]]> 2021-04-10 03:36:54 UTC https://padlet.com/helmisani/researchproblem/wish/1401977586