https://padlet.com/rec11/xmkktkygni5n en-us 2016-06-27 22:01:08 UTC 2023-04-08 16:58:04 UTC hello@padlet.com rec11 https://padlet.com/rec11/xmkktkygni5n/wish/115601708 Liver enzymes break up peroxide, produced by cells, in your body. But how can we prove that it is being broken down? What is produced after the peroxide is broken down? How do we know? In this lab, I am going to try and prove that liver enzymes break peroxide into oxygen and water.]]> 2016-06-28 15:41:13 UTC https://padlet.com/rec11/xmkktkygni5n/wish/115601708 rec11 https://padlet.com/rec11/xmkktkygni5n/wish/115601752 If liver enzymes break down hydrogen peroxide into oxygen and water, you should be able to prove that the product is oxygen by proving its flammability. ]]> 2016-06-28 15:41:49 UTC https://padlet.com/rec11/xmkktkygni5n/wish/115601752 rec11 https://padlet.com/rec11/xmkktkygni5n/wish/115601814 • Test tubes
• Test tube holder  
• Liver 
• Water 
• Hydrogen peroxide 
• Bunsen burner 
• Wood splints 
• Matches ]]> 2016-06-28 15:42:41 UTC https://padlet.com/rec11/xmkktkygni5n/wish/115601814 rec11 https://padlet.com/rec11/xmkktkygni5n/wish/115601930 1. Put five test tubes in the test tube holder
2. Fill the fifth test tube with water until you reach the 2 cm mark
3. Add a piece of liver to the fifth test tube 
4. Light a Bunsen burner and put test tube number five over it 
5. Fill the first test tube with water until you reach the 2 cm mark
6. Add a piece of liver to the first test tube
This will be the control. The liver enzymes should not react to the water. 
7. Fill the second test tube with peroxide until you reach the 2cm mark 
8. Add a piece of liver to the second test tube
Peroxide is our substrate. White bubbles should start to form in the test tube
9. Fill the third test tube with peroxide until you reach the 2cm mark
10. Light a wooden splint on fire 
11. After the splint is glowing red, blow out the fire
12. Slowly put the red-hot splint into the third test tube 
The splint should have no reaction to the peroxide because peroxide is not flammable. 
13. Fill the forth test tube with peroxide 
14. Add a piece of liver 
This tube should have the same reaction as test tube number two.
15. Light a wooden splint on fire
16. After the splint is glowing red, blow out the fire 
17. Slowly put the red-hot splint into the fourth test tube 
The splint should catch on fire because of the oxygen that formed in the tube (pure oxygen in the presence of a spark creates a fire). 
18. Add peroxide to test tube number 5
There should be no reaction (the high temperature should have denatured the enzymes).]]> 2016-06-28 15:44:00 UTC https://padlet.com/rec11/xmkktkygni5n/wish/115601930 rec11 https://padlet.com/rec11/xmkktkygni5n/wish/115602483 2016-06-28 15:51:43 UTC https://padlet.com/rec11/xmkktkygni5n/wish/115602483 rec11 https://padlet.com/rec11/xmkktkygni5n/wish/115602695 1. What are enzymes?  What are their roles in chemical reactions? Enzymes are biological catalysts that increase the rate of chemical reaction by lowering the level of activation energy necessary to start the reaction. Enzymes speed up chemical reactions. They do this by bringing the reactants into close proximity and facilitating their interaction.

2. Describe what happened to test tube 2 when hydrogen peroxide was added to the liver. What gas was produced?  What liquid was left behind? When hydrogen peroxide was added to test tube number two, white bubbles began to form. Oxygen was produced and water was left behind.

3. What effect does the red hot wood have on test tube 4? WHY is the flame being produced? The red hot wood caught on fire; The flame was produce because of the pure oxygen. Pure oxygen is flammable. When the spark was in the presence of the pure oxygen, it caught on fire. 

4. What effect does temperature have on the enzymes (Test tube 5)? The high temperature denatured the enzymes. After the liver was boiled it was no long able to react with the peroxide.]]> 2016-06-28 15:55:59 UTC https://padlet.com/rec11/xmkktkygni5n/wish/115602695 rec11 https://padlet.com/rec11/xmkktkygni5n/wish/115602751 Conclusion: If liver enzymes break down hydrogen peroxide into oxygen and water, I can prove that the product is oxygen by proving flammability. I was able to prove this with test tube four. The reaction of the wood splint to the produced oxygen proved flammability. Test tube two proved that there is a reaction between liver enzymes and peroxide. Test tube three proved that peroxide is not flammable. Test tube four proved that the reaction between the liver enzymes and the peroxide created oxygen and water. Test tube five proved that temperatures that are too high can denature enzymes.]]> 2016-06-28 15:56:53 UTC https://padlet.com/rec11/xmkktkygni5n/wish/115602751