The Portuguese wolf sub-population in the south of the Douro river is fragmented and isolated from the rest of the Iberian wolf population due to geographic, ecological and social barriers.
Conflicts with animal husbandry, negative attitudes, poaching, a lack of wild prey and habitat loss are some of the main threats.

This project (about 1,639,036euros) aims reduce conflicts, diminish poaching and human-caused fires and increase availability of wild prey for the wolf.

The introduction has various origins:  to provide greater yield of inland waters for sport fishing and human feed, escape or release of aquaculture specimens and inadvertently through goods from other countries.

Examples: Sunfish - Lepomis gibbosus (photo), carp (Cyprinus carpio) and black bass (Micropterus salmoides).

As for the average value of the maximum air temperature, in this period, it was "the highest since 1931", that is, in the last 86 years.

However, the biggest problem is not in the occurrence of episodes of greater drought, but in the frequency and intensity with which they occur (more frequent, more intense and with higher temperatures), which begin to worry the researchers.

Regarding forest areas in Portugal, the southern region should be the most affected by the reduction of available water with a consequent reduction in the area. There is also likely to be a migration of forest species from the south to the north, and from the interior to the coast, with a widening of the season of fires and fires in greater numbers and dimensions. In addition, climate change will continue to have an impact on the annual cycles of many living things. For example, there are birds in Portugal that are already reproducing a few days earlier than usual. There are also species that anticipate the date of return to and from the migrations to the places where they spend the winter.