https://padlet.com/softballemma12/x6lui3shsewm Made with a lightning strike of genius en-us 2018-05-04 16:46:26 UTC 2018-05-04 17:10:11 UTC hello@padlet.com softballemma12 https://padlet.com/softballemma12/x6lui3shsewm/wish/258096540 pGLO is extracted from aqueous Victoria ]]> 2018-05-04 16:50:03 UTC https://padlet.com/softballemma12/x6lui3shsewm/wish/258096540 softballemma12 https://padlet.com/softballemma12/x6lui3shsewm/wish/258097171 Bacterial plasmid is added to plates from E. coli to pGlo gene ]]> 2018-05-04 16:52:07 UTC https://padlet.com/softballemma12/x6lui3shsewm/wish/258097171 softballemma12 https://padlet.com/softballemma12/x6lui3shsewm/wish/258097945 Heat shock is used, and bacteria will now have characteristics from the pGLO because of the presence of arabinose ]]> 2018-05-04 16:54:30 UTC https://padlet.com/softballemma12/x6lui3shsewm/wish/258097945 softballemma12 https://padlet.com/softballemma12/x6lui3shsewm/wish/258098698 colonies are removed and put into test tubes then put away over night to use for the next lab period. Culture is added to the tube and goes into the centrifuge. A pellet then forms and the supernatant is poured out and the pallet remains. ]]> 2018-05-04 16:56:28 UTC https://padlet.com/softballemma12/x6lui3shsewm/wish/258098698 softballemma12 https://padlet.com/softballemma12/x6lui3shsewm/wish/258101440 TE soultuion is added and spun again, then lysosome is added. then freeze ]]> 2018-05-04 17:04:21 UTC https://padlet.com/softballemma12/x6lui3shsewm/wish/258101440 softballemma12 https://padlet.com/softballemma12/x6lui3shsewm/wish/258101993 The sample is thawed and spun another time. the supernatant now contains DNA with the GFP protein. The HIC chromatography column is prepared for tomorrows lab period.  ]]> 2018-05-04 17:06:05 UTC https://padlet.com/softballemma12/x6lui3shsewm/wish/258101993