https://padlet.com/Enun_Squared/x2n700tbd5bw en-us 2016-03-08 07:40:18 UTC 2023-01-24 03:25:15 UTC hello@padlet.com Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99577743 2016-03-08 07:47:43 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99577743 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99577814 2016-03-08 07:48:19 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99577814 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99578132 Can the insertion of the GFP gene from bioluminescent jellyfish into e. coli change/transform e. coli into a bioluminescent form/phenotype by expression of the GFP gene into the green fluorescent protein that glows green? ]]> 2016-03-08 07:49:41 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99578132 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99578681 Yes, the insertion of the GFP gene from bioluminescent jellyfish into e. coli can change/transform e. coli into a bioluminescent form/phenotype by expression of the GFP gene into the green fluorescent protein that glows green.]]> 2016-03-08 07:52:35 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99578681 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99578797 The evidence we collected was based off of the observations we saw from the colonies grown on the LB/amp/agar plate, which totaled to one colony. From there we could determine the amount of pGLO DNA spread, which can be calculated by multiplying the total amount of DNA used by the fraction of DNA used, in this experiment it came out to around 0.2. Using these two numbers, the transformation efficiency can be calculated, as it is the total number of colonies growing on the agar plate divided by the amount of DNA spread on the agar plate, which totaled to 6.25 (transformants/ug). ]]> 2016-03-08 07:53:19 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99578797 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99579213 Since we were able to successfully produce a colony that had expressed the GFP gene from the bioluminescent jellyfish, it proves that it is possible for bacteria to produce traits from other species. Therefore, our guiding claim was correct.]]> 2016-03-08 07:55:47 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99579213 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99579858 2016-03-08 08:00:04 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99579858 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99581622 Amount of pGLO DNA (ug) = (concentraton of DNA) x (volume of DNA) = 10 x .008 = .8 ]]> 2016-03-08 08:14:32 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99581622 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99582042 Fraction of DNA used = Volume Spread on LB/amp plate/Total Volume in Test Tube =100/510 = .196]]> 2016-03-08 08:18:12 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99582042 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99582875 DNA spread = (total amount of DNA) x (fraction of DNA) = .8 x .196 = .16 ug]]> 2016-03-08 08:23:46 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99582875 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99583366 2016-03-08 08:27:00 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99583366 Enun_Squared https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99583972 Transformation efficiency = Number of Colonies/Amount of DNA spread on agar plate = 1/.16 = 6.25 transformants/ug]]> 2016-03-08 08:32:06 UTC https://padlet.com/Enun_Squared/x2n700tbd5bw/wish/99583972