Upstream processing before the bioreactor by Sari https://padlet.com/sari_loppela_rauha/wn77oog68ftu Questions made in pairs en-us 2017-03-31 08:09:15 UTC 2025-12-01 20:47:27 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/Lightdecrease.png Mammalian cell https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063266 How do you prepare a medium for mammalian cells on industrial scale?]]> 2017-04-06 07:44:14 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063266 Can you show an example of a medium preparation and optimization process? What are the main arts to master? https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063287 2017-04-06 07:44:21 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063287 Freezing https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063336 How long will the cells survive in frozen state?]]> 2017-04-06 07:44:37 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063336 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063560 What is the optimal pH for a mammal sterilisation?]]> 2017-04-06 07:45:46 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063560 Heating https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063706 What is the optimal temperature if you are using the heating method for sterilizing ?]]> 2017-04-06 07:46:14 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063706 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063736 Which of the sterilisation systems are the most effective, steam, heating or filttion?]]> 2017-04-06 07:46:24 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063736 Term https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063808 Which is the correct term when talking about cell suspension: melt or thaw?]]> 2017-04-06 07:46:48 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063808 AGM https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063874 Is it necessary to remove the foreign cell material e.g. endotoxins after sterilization?]]> 2017-04-06 07:47:15 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063874 Why are antibiotics added to the culture medium? https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063914 2017-04-06 07:47:29 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165063914 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064070 What is the temperature during the sterilization of a bioreactor?]]> 2017-04-06 07:48:22 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064070 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064235 Can pure oxygen be used instead of filtered air and what are the costs?]]> 2017-04-06 07:49:14 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064235 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064297 What is the most cell-friendly way to melt the cells
?

]]> 2017-04-06 07:49:19 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064297 How big a part of the whole process is the pretreatment? https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064501 2017-04-06 07:50:35 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064501 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064581 What's the most common method of sterilization when dealing with mammalian cells]]> 2017-04-06 07:51:06 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064581 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064670 In which cases is the HTST method used]]> 2017-04-06 07:51:38 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165064670 JEP https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165065862 What is the safest way to thaw the cells?]]> 2017-04-06 07:59:54 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165065862 https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165837860 What are the uppstream processes for bacterial cells which produce enzymes?

]]> 2017-04-11 09:22:45 UTC https://padlet.com/sari_loppela_rauha/wn77oog68ftu/wish/165837860