CRISPR: Activity by EUN Partnership aisbl

Alessia. Italy

alessiabrunetta — 2019-04-23 09:37:58 UTC

Here is my work

Geraldine Ireland

coughlag1 — 2019-04-23 12:58:03 UTC

Q1 Methionine
Q2 Proteins are made from mRNA and not original copy of the DNA

Julija, Austria

2019-04-23 13:17:23 UTC

My word document is attached to this comment:

Aishling, Ireland

2019-04-23 13:26:08 UTC

Attached below! A good little recap of Transcription and Translation for me :D

Renata,Poland

2019-04-23 13:29:22 UTC

DNA : template standard TACAGAGGATCAGTT

mRNA : AUG-UCU-CCU-AGU-CAA

tRNA : UAC-AGA-GGA-UCA-GUU

normal : MetSerProSerGln

mutant : MetCysProSerGln

mutant mRNA :AUG-UGU-CCU-AGU-CAA

mutant DNA coding : ATGTGTCCTAGTCAA

1. methionine

2. It is said that the genetic code is indirect, which means that DNA templates are never directly used to "stack" amino acids.

Ankica,Croatia

2019-04-23 13:31:46 UTC

My work:
1-Methionine
2-Proteins are made from mRNA

Fabiola, Italy

2019-04-23 13:36:44 UTC

Question 1: Methionine
Question 2:All proteins don't have this amino as their firts because proteins are made from RNA and they aren't a copy of DNA.

2019-04-23 14:09:05 UTC

2019-04-23 14:09:21 UTC

Karela Georgia, Greece

geokar3 — 2019-04-23 16:13:33 UTC

DNA: TACAGAGGATCAGTT

mRNA: AUG-UCU-CCU-AGU-CAA

tRNA: UAC-AGA-GGA-UCA-GUU

normal: MetSerProSerGln

mutant: MetCysProSerGln

mutant mRNA: AUG-UGU-CCU-AGU-CAA
mutant DNA: ATGTGTCCTAGTCAA

1. Methionine
2. All proteins don't have this amino acid as their first because they are made from RNA and they are not copies of DNA.

Miquel, Picassent

2019-04-23 17:00:46 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA

DNA Template strand: TAC AGA GGA TCA GTT

mRNA Codons:
AUG UCU CCU AGU CAA

tRNA Anticodons:
UAC AGA GGA UCA GUU

Amino acid sequence

Normal PDSTB1:
Met-Ser-Pro-Ser-Gln

Mutant PDSTB1:
Met-Cys-Pro-Ser-Gln

Mutant mRNA. AUG UGU CCU AGU CAA

Mutant DNA Coding strand ATG TGT CCT AGT CAA

1. Methionine (Met) (M)

2. Because this amino acid is removed after protein synthesis in ribosomes.

Adela Muresan, ROMANIA

muresan_gabriela_adela — 2019-04-23 17:44:47 UTC

1 Methionine
2 Proteins are made from mRNA and not original copy of the DNA

Carina, Ireland

2019-04-23 18:15:20 UTC

Lacramioara,Romania

2019-04-23 19:15:32 UTC

1. Methionine
2.Proteins are made from RNA.

2019-04-23 19:20:29 UTC

Osman Serhat ACAR-Turkey

Valentina, Romania

2019-04-23 22:47:39 UTC

1.(Met) (M) Methionine
2. Because they are made from RNA and they are not copies of DNA

Abdullah KÖROĞLU, Turkey

2019-04-24 06:32:44 UTC

Maria-Italy

mariaelena_tozzi68 — 2019-04-24 07:43:18 UTC

1-Methionine

Methionine is the only amino acid specified by just one codon, AUG. The stop codons are UAA, UAG, and UGA. They encode no amino acid. The ribosome pauses and falls off the mRNA.
2- Because they are made from RNA and they are not copies of DNA

Mian. Barcelona

2019-04-24 13:28:16 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA

DNA Template strand: TAC AGA GGA TCA GTT

mRNA Codons:

AUG UCU CCU AGU CAA

tRNA Anticodons:

UAC AGA GGA UCA GUU

Amino acid sequence

Normal PDSTB1:

Met-Ser-Pro-Ser-Gln

Mutant PDSTB1:

Met-Cys-Pro-Ser-Gln

Mutant mRNA. AUG UGU CCU AGU CAA

Mutant DNA Coding strand ATG TGT CCT AGT CAA

1. Methionine (Met) (M)

2. Proteins are made from RNA.

Raja Nadeem Altaf,Pakistan,Islamabad:

clinicalresearcher2019 — 2019-04-24 13:41:13 UTC

DNA Coding Strand
-ATGTCTCCTAGTCAA-
DNA Template Strand
-TACAGAGGATCAGTT-
mRNA Codon
-UACAGAGGAUCAGUU-
tRNA Anticodon
-AUGUCUCCUACUGAA-

mRNA is Mutant

Jordi, Barcelona

2019-04-24 14:44:47 UTC

Joan, Barcelona

2019-04-24 20:02:57 UTC

1. Methionine (codon AUG)

2. Because proteins are synthesized in ribosomes from mRNA.

Luminita, Ro

adi_luminita — 2019-04-25 08:28:10 UTC

1.(Met) (M) Methionine
2. Because they are made from RNA and they are not copies of DNA

Gamze Siper/Turkey

gamzesiper — 2019-04-25 10:30:34 UTC

Metionin amino acid starts codon encode and because proteins made of RNA.

Hakan

2019-04-25 12:27:03 UTC

DNA Coding strand:
ATG TCT CCT AGT CAA

DNA Template strand: TAC AGA GGA TCA GTT

mRNA Codons:

AUG UCU CCU AGU CAA

tRNA Anticodons:

UAC AGA GGA UCA GUU

Amino acid sequence

Normal PDSTB1:

Met-Ser-Pro-Ser-Gln

Mutant PDSTB1:

Met-Cys-Pro-Ser-Gln

Mutant mRNA. AUG UGU CCU AGU CAA

Mutant DNA Coding strand ATG TGT CCT AGT CAA

1. Methionine (Met) (M)

2. Proteins are made from RNA.

Fethi Ahmet,TURKEY

2019-04-25 19:34:02 UTC

1. The AUG codon encoding the methionine amino acid.
2. Because these amino acids are essential for the synthesis mechanism for all proteins. In order to become active protein, changes in structure are necessary. RNA is the code that read and understand the proteins.

Annalaura Sassari

2019-04-26 09:11:43 UTC

Àlex Asensio (Girona, Catalonia)

2019-04-26 10:13:27 UTC

António Branco, Portugal

2019-04-26 16:07:41 UTC

1 - Methionine
2 - Proteins are made out of RNA

Olivera, Montenegro

OliveraOlja — 2019-04-26 22:24:50 UTC

DNA Coding strand - ATG-TCT-CCT -AGT-CAA

(A=T, C=G)

DNA TEMPLATE STRAND -TAC-AGA-GGA-TCA-GTT

mRNA CODONS -AUG- UCU-CCU-AGU-CAA-

(A=U, C=G)

tRNA ANTICODONS -UAC-AGA-GGA-UCA- GUU-

AMINO ACID SEQUENCE

NORMAL PDSTb1: MET SER PRO SER GLN (MSPSQ)

MUTANT PDSTb1: MET CYS PRO SER GLN (MCPSQ)

MUTANT mRNA AUG-UGU-CCU-AGU-CAA

MUTANT DNA CODING STRAND. –ATGTGTCCTAGTCAA--

QUESTIONS

1: METHIONINE (Met)

2: Because this amino acid is removed after protein synthesis in ribosomes.

Maria Gonçalves, Portugal

m3nevesg — 2019-04-27 00:11:31 UTC

Methionine

They are made from RNA

Katerina, Czech republic

2019-04-27 06:19:11 UTC

My answers:

Maria S., Italy

aquilone331 — 2019-04-27 14:48:18 UTC

Uta

steinel_schrenk — 2019-04-28 00:22:47 UTC

sorry I am no science teacher. I like more P4C

Leyla, Turkey

2019-04-28 08:57:49 UTC

Dilek/ Turkey

2019-04-28 17:26:17 UTC

DNS Coding strand: ATG TCT CCT AGT CAA
DNA Template strand: TAC AGA GGA TCA GTT
mRNA Codons: AUG UCU CCU AGU CAA
tRNA Anticodons: UAC AGA GGA UCA GUU
Normal PDSTB1: MET SER PRO SER GUN
Mutant PDSTB1: MET CYS PRO SER GUN
Mutant mRNA: AUG UGU CCU AGU CAA
Mutant DNA Coding strand: ATG TGT CCT AGT CAA
Q1: MET
Q2: Their structure is amino acid

Ionica , Ro

2019-04-28 17:46:14 UTC

Methionine
and proteins are made out of RNA

P Campos, Portugal

patricia_campos1 — 2019-04-28 21:35:35 UTC

halil kaya türkiye

2019-04-29 00:03:39 UTC

Many views become clear and students often question their opinions. They also become more tolerant of other opinions and develop more reflective and problem awareness

Cristina Romana

2019-04-29 13:55:06 UTC

Cristina Romania

2019-04-29 13:55:08 UTC

Cristina Romania

2019-04-29 13:55:10 UTC

Proteins are made from RNA and they not copy DNA.

Snežana, Croatia

kranjec051 — 2019-04-29 17:21:38 UTC

Sorry, I am not a science teacher.

Jelena ,Montenegro

2019-04-29 19:52:19 UTC

My answers
DNA Coding strand - ATG-TCT-CCT -AGT-CAA
(A=T, C=G)

DNA Template strand -TAC-AGA-GGA-TCA-GTT

mRNA codons -AUG- UCU-CCU-AGU-CAA-
(A=U, C=G)

tRNA anticodons -UAC-AGA-GGA-UCA- GUU-

Amino acid sequence

Normal PDSTb1: MET SER PRO SER GLN (MSPSQ)

Mutant PDSTb1: MET CYS PRO SER GLN (MCPSQ)

Mutant mRNA AUG-UGU-CCU-AGU-CAA
Mutant DNA Coding strand –ATGTGTCCTAGTCAA--

Answers and questions

1. Methionine (Met)

2. Because this amino acid is removed after protein synthesis in ribosomes.

Žaklina K. Croatia

zaki_kvesic — 2019-04-29 20:33:28 UTC

I am not science teacher.

Bea, Italy

2019-04-30 14:30:50 UTC

DNA templ. strand: TAC AGA GGA TCA GTT
mRNA cod.: UAC AGA GGA UCA GUU
tRNA anticod.: AUG UCU CCU AGU CAA
met-ser-prol-ser-glutam
Mutation by bubstitution:
AUG UGU CCU AGU CAA (met-cys-prol-ser-glutam)
mRNA: UAC ACA GGA UCA GUU
DNA: ATGTGTCCTAGTCAA
Questions: 1) Met; 2) Because the amino acid is removed after proteine synthesis

Grace,Sicily

grace_garofalo — 2019-05-01 05:57:18 UTC

-Met
-proteins are made from RNA

Grace,Sicily

grace_garofalo — 2019-05-01 05:58:35 UTC

-Met
-proteins are made from RNA

Adriana, Romania

2019-05-01 13:14:54 UTC

I am not a science teacher.

Josip, Croatia

bazamaterijala — 2019-05-01 13:19:02 UTC

I tried. It took me a while...

Ivica G., CRO

ivica_gregurec — 2019-05-01 16:54:33 UTC

1 - Methionine
2 - Proteins are made out of RNA

Núria, Barcelona

nuriagflor1 — 2019-05-01 18:27:59 UTC

I am not a science teacher

anhlicyer — 2019-05-01 19:04:22 UTC

Sevda-TURKEY

Sevdakurupelit — 2019-05-02 07:59:50 UTC

Sorry, I am not a science teacher.

Markéta, CZ

2019-05-02 13:10:01 UTC

1. Methionine
2. The start codon is recognized by a small ribosomal subunit that scans the mRNA strand and attempts to associate with the start-codon sequence.
In 90% of cases, the first AUG codon is recognized, and in a minority of cases, another codon of this type is recognized...

Markéta, CZ 2

2019-05-02 13:12:35 UTC

Ayten, Turkey

2019-05-02 15:44:04 UTC

1. Methionine
2. AUG is the start codon and proteins are the results of RNA activity

2019-05-02 19:49:58 UTC

Sinéad , Barcelona

2019-05-02 19:55:45 UTC

Vasilica,Romania

simionoviciv — 2019-05-03 06:05:48 UTC

I'm sorry, I'm not a science teacher

Magdalena, Austria

2019-05-03 12:07:57 UTC

1. MET: Methionine

2. Not all proteins have this amino acid as their first because they are made from mRNA.

Kenneth - USA, Georgia

2019-05-03 15:55:27 UTC

I hope someone will read and respond to my post. Since my background is Mathematics and not science I found this activity to be very difficult and had to do some outside research and I'm not sure my conclusions are correct. Most everyone just had one answer for the mutant RNA but since you were replacing one serine with a cycseine, aren't there two possibilities? Then since there are two possible codes for the cysteine don't there become four possibilities for the mutant mRNA and DNA coding strand? I would appreciate input.

Cecilia, Italy

2019-05-03 17:08:57 UTC

I'm not have so high competence in science

Arif Soğukpınar, Turkey

2019-05-03 18:06:11 UTC

sorry, but it is too hard for me to understand. I'm an English teacher.

Marcin, Poland

emce_1980 — 2019-05-03 18:44:12 UTC

This was the haard one :)
DNA coding strand:
-- A T G - T C T - C C T - A G T - C A A –

DNA template strand:
-- T A C - A G A - G G A - T C A - G T T –

mRNA codons
-- A U G – U C U – C C U – A G U – C A A –

tRNA anticodons:
-- U A C – A G A – G G A – U C A – G U U –

amino acid sequence: Met Ser Pro Ser Val Gln

Mutant PDSTb1: Met Cys Pro Cys Gln

Mutant mRNA AUG UGU CCU UGU CAA

Mutant DNA Coding strand ATG TGT CCT TGT CAA

Questions:

1. The start codon codes for Metionine

2. Because they may be modified

Eva, Czech republic

efkakuc — 2019-05-05 14:11:17 UTC

Sorry, I'm not a science teacher. It is too hard for me to understand.

Leah, Austria

2019-05-05 15:36:35 UTC

Question 1: The start codon encodes Methionin.
Question 2: Not every protein contains Methionin as their first amino acid, because - after translation - enzymes often remove the initial parts of a polypeptide.

Sandra, CRO

knjiznica_tsck — 2019-05-06 08:09:48 UTC

This task is to hard for me. I read all the answers but I don't know how to write my own.

2019-05-06 20:16:34 UTC

Methionine
Proteins are made out of RNA

Beata, Poland

2019-05-06 21:24:36 UTC

DNA Coding strand:
ATG-TCT-CCT-AGT-CAA

DNA Template stand:
TAC-AGA-GGA-TCA-GTT

mRNA Codons:
AUG-UCU-CCU-AGU-CAA

t RNA Anticodons:
UAC-AGA-GGA-UCA-GUU

Normal PDSTb1:
Met-Ser-Pro-Ser-Gly

Mutant PDSTb1:
Met-Cys-Pro-Ser-Gly

Mutant mRNA:
AUG-UGU-CCU-AGU-CAA

Mutant DNA Coding strand:
ATG-TGT-CCT-AGT-CAA

1. Methionine
2. All proteins don't have this amino acid as their first because they are made from RNA and they are not copies of DNA.

Joanna, Poland

2019-05-07 12:53:10 UTC

M

2019-05-07 20:56:06 UTC

MUSTAFA

2019-05-07 20:56:07 UTC

NA Coding strand:
ATG TCT CCT AGT CAA

DNA Template strand: TAC AGA GGA TCA GTT

mRNA Codons:

AUG UCU CCU AGU CAA

tRNA Anticodons:

UAC AGA GGA UCA GUU

Amino acid sequence

Normal PDSTB1:

Met-Ser-Pro-Ser-Gln

Mutant PDSTB1:

Met-Cys-Pro-Ser-Gln

Mutant mRNA. AUG UGU CCU AGU CAA

Mutant DNA Coding strand ATG TGT CCT AGT CAA

1. Methionine (Met) (M)

2. Proteins are made from RNA.

Sabriye Çelik, Turkey I am not science teacher and I can try

sayarig01 — 2019-05-07 21:52:04 UTC

1, Metionine
2,The first AUG codon

Elina

2019-05-09 07:44:59 UTC

Elina

2019-05-09 07:45:01 UTC

DNA template strand

TAC AGA GGA TCA GTT

mRNA codons

AUG UCU CCU AGU CAA

tRNA Anticodons

UAC AGA GGA UCA GUU

Amino acid sequence

Normal PDSTb1

Met-Ser- Pro- Ser- Gln

Mutant PDSTb1

Met – Cys- Pro-Ser- Gln

Mutant mRNA

AUG-UGU- CCU-AGU- CAA

Mutant DNA coding strand

ATG-TGT- CCT-AGT- CAA

1. Methionine

2. Proteins are made from RNA

alina popa romania

popalina21 — 2019-05-10 13:02:16 UTC

very interesting aproach of biochemistry

Gabriella, Italy

gabriella — 2019-05-10 13:27:04 UTC

DNA template strand:
TACAGAGGATCAGTT
mRNA codons:
AUG-UCU-CCU-AGU-CAA
tRNA Anti-codons:
UAC-AGA-GGA-UCA-GUU
normal PDSTß1: MetSerProSerGln
mutant PDSTß1: MetCysProSerGln
mutant mRNA: AUG-UGU-CCU-AGU-CAA
mutant DNA coding strand: ATGTGTCCTAGTCAA
1- Methionine
2- Proteins are translated from Rna, so it does not depend on a Dna strand

Franklan, Malta

2019-05-11 15:59:02 UTC

I found this very hard to do and I didnt undertstand what i actually have to do. I tried to search on internet but still no success :(

Rosana, Spain

rosanasanchisrubio — 2019-05-12 06:47:18 UTC

Austria, SJ

2019-05-12 17:12:05 UTC

Unfortunately I was not able to open the image in a word-document, so I am answering here:

DNA template strand:
TACAGAGGATCAGTT

mRNA codons:
AUG-UCU-CCU-AGU-CAA

tRNA Anti-codons:
UAC-AGA-GGA-UCA-GUU

normal PDSTß1: MetSerProSerGln

mutant PDSTß1: MetCysProSerGln

mutant mRNA: AUG-UGU-CCU-AGU-CAA

mutant DNA coding strand: ATGTGTCCTAGTCAA

1. Methionine
2. Because the proteins are possesed after transition...

Silvana, Italy

2019-05-12 22:19:16 UTC

Ioana, Romania

2019-05-13 17:41:34 UTC

1. Methionine
2. Protein synthesis is due to the translation of genetic information
Incorrect insertion of an amino acid in the protein chain (mutation - occurrence of some diseases).

ÖZDEN COŞAR TÜRKİYE

ozdencosar — 2019-05-14 12:06:40 UTC

VERY İNTERESTİNG

Anne

anne_badiola — 2019-05-14 12:25:53 UTC

I didn't really understand how to do the activity so I really couldn't do it.

Francesc, Catalunya

2019-05-14 16:30:42 UTC

Francesc, Catalnya

2019-05-14 16:32:10 UTC

Maria, Ireland

ariasteinbo — 2019-05-14 19:11:47 UTC

Magdalena, Poland

2019-05-14 22:09:34 UTC

I had also problems to open in a word-document, so I am posting my answers below:

DNA template strand: … TACAGAGGATCAGTT …

mRNA codons: AUG-UCU-CCU-AGU-CAA

tRNA Anti-codons: UAC-AGA-GGA-UCA-GUU

normal PDSTß1: MetSerProSerGln

mutant PDSTß1: MetCysProSerGln

mutant mRNA:
AUG-UGU-CCU-AGU-CAA

mutant DNA coding
strand: ATGTGTCCTAGTCAA

1. The start codone
encode: Methionine
2. Because there
are post transcription modifications (proteins can vary)

Katia, Romania

2019-05-15 05:02:32 UTC

The aminoacid coded is metionine. Proteins are made form RNA

Michael, Austria

2019-05-15 11:38:16 UTC

1. Methionine
2. The star codon is AUG, proteins are the result of RNA.

Máire, Ireland

2019-05-15 17:08:33 UTC

DNA template strand: TACAGAGGATCAGTT
mRNA codons: AUG UCU CCU AGU CAA
tRNA Anti-codons: UAC AGA GGA UCA GUU
Normal PDSTß1: MSPSQ
Mutant PDSTß1: MCPSQ
Mutant mRNA:
AUG UGU CCU AGU CAA
Mutant DNA coding
strand: ATGTGTCCTAGTCAA

1. Methionine
2. Possible historic mutations

Viktoria, Vienna

2019-05-16 15:47:19 UTC

1.) Methionine
2.) Because of post-translational modifications

DNA template strand: TACAGAGGATCAGTT
mRNA Codons:
AUGUCUCCUAGUCAA
tRNA Anticodes:
UACAGAGGAUCAGUU
Normal PDSTß1:
met-ser-pro-ser-qin
Mutant PDSTß1:
met-cys-pro-ser-qin
Mutant mRNA:
AUGUGUCCAGUCAA
Mutant DNA coding strand:
ATGTGTCCTAGTCAA

Mariangela Fabiana Gramegna, Italy

facararolina — 2019-05-16 17:06:55 UTC

Methionine
Possible mutations

Adina Geczi, Romania

2019-05-19 19:03:04 UTC

1. Methionine
2. Proteins are made from mRNA

2019-05-23 17:48:57 UTC

Pratima Singh, India
TACAGAGGATCAGTT
AUG-UCU-CCU-AGU-CAA
UAC-AGA-GGA-UCA-GUU
MetCysProSerGln
AUG-UCU-CCU-AGU-CAA
ATGTGTCCTAGTCCAA
Methionine

2019-06-22 17:13:57 UTC

Nilüfer Parsak Adana Turkey