(And what was the species you used as an example called again?)

https://browser.planteome.org/amigo/term/PO:0009013

We have worked hard to describe the various cell types and would love feedback from the community

taking together all the millions of years in evolution: have we reached the entire set of cell-types and plant structures across time, or is there still room for innovation?

What are the functions of the most common, highly conserved genes? Is there an accurate/current list?

Excelent talk! I have a question about gene modules: to what extent is it possible to build a gene module that defines a cell's identity, especially when studying how this specific cell type diverges in terms of evolution?

Are cell-specific markers the same across different species?

Actually, cell division occurs in meristem. After division, how do the cells develop into different types?

When comparing cell types between species using a subset of genes (e.g., one-to-one orthologs) have you assessed whether this subset equally preserves cell type identity/ structure of the clusters within each species? I'm wondering if the level of differences or similarities observed across the species could be influenced by gene selection rather than reflecting true biological divergence.

Hi Ken! In the Guillotin paper I noticed that there is a large number of Arabidopsis cells labeled endocortex that are a bit far from endodermis and cortex. I was wondering if this could reflect a challenge to define cell type even in the well-studied species Arabidopsis... what are your thoughts on this?

Would adding protein and metabolite information help with better definition of cell types?

Thanks Kaisa for the talk. Does ABA induce also lignin deposition in exodermis? If so, when there is an ABA-induced exodermis (based on suberin) in multiple cortical layers, is lignin deposition in same layers? And can you see the polar lignin cap?

It was very informative session and I got some new ideas. We should be very careful about our environment. Thank you speakers.

Could you elaborate on how the distinct transcriptional phenotypes of barrier cell types in chickpea are regulated and whether the observed differences in gene expression are conserved across other legume species?

Great talk. I have a question to Kaisa, you mentioned MYBs regulating suberin as downstream MYBs, shouldn't they be upstream MYBs since they are probably regulating the expression of suberin biosynthetic genes? I may be wrong.

Is root structure involved in shoot or leaf biochemical aspects due to any kind of environmental stress?

Great talk Kaisa, thanks! Is ABA responsible for stablishing the exodermis in those species were you find it without needing the ABA treatment (if there is any such species)?

Is kc9 providing an adaptability response to plants challeneged with a pathogen for example Botrytis? In other words, does the cross-talk has an ecological response?

Do stomata develop the same way in cotyledons and in true leaves?

can you speculate on the nature of the small cells that are hijacked from the stomatal lineage when you apply the chemicals and how do the chemicals relate to the potential stem cell nature of those cells

You mentioned KC9's affinity for MPK6 decreases when MPK6 is pre-activated. Could you elaborate on the potential molecular mechanism behind this reduced affinity? Is it due to conformational changes in MPK6 upon activation, or something else?

It's fascinating to see the crosstalk between developmental and defense signaling. If we knock out the receptor for flg22, would the developmental signaling be able to influence the defense response?

Can reducing the stomata number be taken as a strategy to tackle biotic stress..?

Hello Justin, great to see you again. One naive question. To what extend protein turnover/dynamics can impact your profiling and need to be taken ito consideration when designig your workflow/s?

Hi Justin, thanks for presenting this work. Would there be enough materials in single cells to enrich for the phosphoproteome? Can we add more carrier cells for this purpose?

Thanks for the presentation. What methods yo used to isolate the cortical and endodermis cells? any idea how much proteins/per cell? Thanks.

Is the MALDI technique limited to specific types of plant tissues? Would it also work on a plant callus?

These are all acids, do you control pH of your growth media?

How did you choose the concentrations of citrate etc to use?

Con you recognise from the data cell wall components (polimers) and their modifications?

Is the technology and equioment improving in terms of spatail resolution? Is it possible to combine this with those techniques used in expansion microscopy to increase inderectly spatial resolution?

Great talk, Jazz! I wonder if you think that metabolites that have striking effects like citric acid are being percibed as signalling molecules by different celltypes or is it mostly their contribution to overall metabolism (ie carbon source)

What could be the reason behind less or no H2O2 at down or tip portion of the plant roots during plant growth

Beatiful results and imaging! Considering the cell type specific signalling of BR en roots, the results you showes are in cortex or epidermal cells?

Might this upper-lower levels of BR signalling might define in some way, for example interacting with other hormones the transition to cell expansion?

Are all nuclei of the same character?

to Elliot: can you test for de novo organ formation and assess the potential role of informational molecules such as RNAs using regeneration experiments, following the Acetabularia paradigm from the 1930s?

Hi Alex, are those ACRs associated with or affected by any histone modifications?

For Elliot: thanks for showing such a unusual system, reminding one a bit of the "blob" that has become popular in schools. Polarization in plant cells plays an important role. Do you know much about what maintains polarization here, for instance for driving the oriented motion of chloroplasts?

Thank you both for terrific presentations! Alex: can you comment on hurdles / enablers for high throughput single cell experiments, i.e. 100+ samples

Marina - amazing work! Could you identify/ predict what are the genes or TFs activating the ERS? Do you think that there can be mutants identified where the plants fail to activate ERS?

nice talk! in your case, environmental stress is abitic stress, could this possibely be biotic stress?

Hi Marina, great talk. Have you checked at the stemness of those ERS cells with any marker? Does drought stress increase the stemness of cell types?

great talk! what about overexpressing the ERS genes in the root? will that leads to more drought resistance?

Great talk Marina! It seemed to me the root cap has mostly ERS cells instead of the two states. You didnt focuss on these cells in your talk but if I got it right about them could it be those cells are more steady responders?

Morning. Were these ERS genes entirely a novel discovery in this scRNA experiment, or could a bulk RNAseq experiment have been sufficient to discover them?