Histoplasmocytosis by Foong May Yeong https://padlet.com/fyeong/vjc4gkl0o1fu Class discussion en-us 2016-08-19 09:21:22 UTC 2016-09-25 06:41:24 UTC hello@padlet.com Hypothesis fyeong https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884904 2016-08-19 09:23:31 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884904 Methods fyeong https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884910 2016-08-19 09:23:36 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884910 Findings fyeong https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884912 2016-08-19 09:23:39 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884912 Conclusions fyeong https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884922 2016-08-19 09:23:56 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884922 Strengths fyeong https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884928 2016-08-19 09:24:05 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884928 Weaknesses fyeong https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884937 2016-08-19 09:24:11 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/118884937 https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119084878 Histoplasma cell wall a-(1,3)-glucan blocks PRR recognition and facilitates evasion of host detection ]]> 2016-08-22 03:03:51 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119084878 Verify location of a-(1,3)-Glucan around yeast buds using DIC and IF microscopy using labelled antibody https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119084968 2016-08-22 03:05:07 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119084968 Immunolabeled IF Microscopy and Electron Microscopy to verify colocalization of a-(1,3)-Glucan and b-(1,3)-Glucan https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085232 a-(1,3)-Glucan abt 20-100nm away from cell membrane
b-(1,3)-Glucan abt 0-40nm away from cell membrane]]> 2016-08-22 03:09:03 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085232 https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085510 1) A-(1,3)-glucans reduces dectin-1 recognition of histoplasma yeast, recognition based solely on dectin-1
2) a-(1,3)-glucans suppresses the release of tnf-a by phagocytic cells, reduces activation of macrophages, and reduces phagocytosis of the histoplasma ]]> 2016-08-22 03:12:12 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085510 https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085519 To find out how a-(1,3)-glucan contributes to virulence]]> 2016-08-22 03:12:18 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085519 Study the interaction of dectin-1 and a-(1,3)-glucan https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085689 Express dectin-1 transgene in 3T3 fibroblast cell line and treat cell lines with yeast cells expressing a-(1,3)-glucan and without.]]> 2016-08-22 03:14:07 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085689 a-(1,3)-Glucan blocks recognition by dectin-1 https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085732 ags1 KO binds to 3T3 cell expressing dectin-1 and dectin-1 localization with antibody labeling

Verified and quantified using IF Microscopy ]]> 2016-08-22 03:14:38 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085732 Mechanism of vriulence mediated by alpha glucan https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085746 2016-08-22 03:14:47 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085746 Immunofl microscopy to localise the position of alpha glucan https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085770 Labelling with gold particle

Ags1 KO and binding with 3T3 cells dectin +/-

Tnf alpha release by P388D1 phagocytes 

ShRNA to reduce expression of both isoforms  of dectin 1 and its role in mediating immune recognition]]> 2016-08-22 03:15:10 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085770 Study functional significance of a-(1,3)-glucan masking by examining TNFa production https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085931 KO of a-(1,3)glucan in yeast cells  

KD of dectin-1 in P388D1 macrophage-like cells and infect with WT and KO yeasts ]]> 2016-08-22 03:17:09 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119085931 Alpha glucans masks beta glucan from dectin receptors and thereby don't activate immune cascade  https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086127 2016-08-22 03:19:37 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086127 a-(1,3)-glucan interferes with recognition of dectin-1 and TNFa production https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086156 TNFa production was reduced to similar levels by KD of dectin-1 in host or by presence of a-(1,3)-glucan]]> 2016-08-22 03:20:00 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086156 Use of immunofluorescencw microscopy to quantify and localise beta and alpha glucans critically https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086290 2016-08-22 03:21:58 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086290 https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086469 Use of invivo model 

The mechanism of interaction of alpha glucan and the host through PRRs

Quantify dectin in protein level after shRNa]]> 2016-08-22 03:24:22 UTC https://padlet.com/fyeong/vjc4gkl0o1fu/wish/119086469