https://padlet.com/maliahee/v49mkbosrntlv7by Write a response to the following: 1. What do you notice about the results of the chromatography activity? What questions/further investigation(s) come to mind? en-us 2022-11-29 13:26:35 UTC 2022-11-29 17:15:34 UTC hello@padlet.com https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402187348 2022-11-29 16:18:53 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402187348 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402202289 The different pigments that are present in the plants were brought out when they reacted with the isopropyl alcohol. Would a different solution work better to display the pigments? ]]> 2022-11-29 16:27:48 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402202289 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402202328 The color variation correlates to the plant pigment brought out by isopropyl alcohol. 
How would other solutions expose a different pigmentation of the plant? ]]> 2022-11-29 16:27:49 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402202328 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402202916 The solvent carried the various pigments up the paper, separating them and making them easier to identify and observe.]]> 2022-11-29 16:28:12 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402202916 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402203191 In the chromatography activity, I noticed both the reddish-brown and the blue pigments traveled up the paper while the green pigment stayed where it was placed, but other colors like yellow and orange traveled away from the green as the solvent moved up the paper. One thing I would test further is if a different solution would change the color or the way a pigment would travel. ]]> 2022-11-29 16:28:22 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402203191 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402203601 I observed that all the different leaves of spinach had a different color in them when left to sit in the alcohol. 
From here, I could start to figure out what causes the difference in those pigments of the leaves and the substances. ]]> 2022-11-29 16:28:38 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402203601 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402204241 2022-11-29 16:29:03 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402204241 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402205225 The pigments brought out from each plant were unique. I would like to run the experiment again with a greater variety of different plants.]]> 2022-11-29 16:29:39 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402205225 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402205815 1. I noticed that there were different hues that show up from the leaf despite the leaf being green
2. I'm thinking that maybe we could do the chromatography test on other plants that have a variety in color.]]> 2022-11-29 16:30:01 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402205815 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402207085 I noticed that all of the pigments except the green from the spinach traveled all the way up the paper. The colors also faded significantly. How would a different solution make the pigments react?]]> 2022-11-29 16:30:47 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402207085 jessicanebeker https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402207324 I observed that the isopropyl alcohol revealed that the different plants had different amounts and levels of colors. I would like to do the experiment again with more plants and different concentrations of the alcohol. ]]> 2022-11-29 16:30:56 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402207324 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402208368 The isopropyl alcohol was able to bring out multiple different pigments in each of the leaves. How does the isopropyl alcohol make these colors visible?]]> 2022-11-29 16:31:34 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402208368 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402208612 The leaves all had different colors or pigments when they were put in the solution. What would happen with the color if you used a different type of solution]]> 2022-11-29 16:31:43 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402208612 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402208657 The majority of the pigments were carried to the top of the paper, indicating that the pigments were highly soluble in the isopropanol. Some of the pigments did separat, likely based on polarity
In the future I would try using different solvents (such as a nonpolar solvent like hexane) to try to isolate pigments with different polarities/solubilities.]]> 2022-11-29 16:31:45 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402208657 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402209239 The pigments were separated into their respective groups as they traveled up the paper. I would want to investigate how the prevalence of the pigments differ between types of leaves.]]> 2022-11-29 16:32:05 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402209239 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402209667 The isopropyl alcohol revealed the hidden pigments, which were different for every sample. The green sample from the spinach  was largely unmoved, while the others were erased and moved up the filter paper. I wonder if a more concentrated or precise way to extract the starting pigments was possible, and if used, would it change the results?]]> 2022-11-29 16:32:21 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402209667 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402209698 The pigments brought out from the greener leaves tended to have way less of the brownish pigments than the purple leaves, which tended to only have the brown pigment or no pigment at all. To repeat this experiment, I would take different leaves from more similarly colored plants, so we can see the minor variations in plants as opposed to taking already very different plants.]]> 2022-11-29 16:32:22 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402209698 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402210286 It was interesting to see the division of color in some leaves and the strong line carried up in other leaves.  I would like to run a more controlled experiment with more types of leaves and pigments. ]]> 2022-11-29 16:32:45 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402210286 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402210651 2022-11-29 16:32:59 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402210651 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402210816 I noticed that the different colors on the paper corresponded with the pigments seen on the leaves, and they traveled up the paper different amounts. If I test this again, I'd use different solvents to see if they'd react similarly.]]> 2022-11-29 16:33:05 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402210816 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402211323 The pigments traveled very high up the paper, and the color of the pigments corresponded with the color of the vegetables. I wonder if the amount of water used will affect the pigments at all. ]]> 2022-11-29 16:33:26 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402211323 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402214015 2022-11-29 16:35:06 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402214015 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402214496 The cabbage pigment was barely visible and the color of the radicchio turned brown. If I were to repeat this experiment, I would use leaves from plants grown in light vs dark environments.]]> 2022-11-29 16:35:24 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402214496 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402216632 2022-11-29 16:36:46 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402216632 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402217927 The pigment of each plant was brought out as they traveled up the paper. I wonder what would happen if we used a different type of solution for this experiment. ]]> 2022-11-29 16:37:37 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402217927 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402218579 2022-11-29 16:38:00 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402218579 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402219191 I noticed that the different pigments traveled different lengths up the paper, and the green pigment had a stronger color while the other plants were more faded.
I would want to try this experiment again with another green pigment plant, but a different shade.]]> 2022-11-29 16:38:20 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402219191 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402222681 2022-11-29 16:40:27 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402222681 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402224018 2022-11-29 16:41:11 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402224018 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402224184 I noticed that the colors traveled up the paper and spread out into several different hues. For example, green expanded into a broader spectrum of yellows and greens.

If I were to redo this experiment I would use plants that release stronger pigments to make it easier to see, as the brown plant’s pigment was almost (visibly) nonexistent.]]> 2022-11-29 16:41:17 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402224184 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402256590  ]]> 2022-11-29 17:01:32 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402256590 https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402271701 2022-11-29 17:10:53 UTC https://padlet.com/maliahee/v49mkbosrntlv7by/wish/2402271701