by https://padlet.com/wall/uvkhaz6vnq en-us 2014-04-14 04:31:04 UTC 2014-04-14 04:31:04 UTC hello@padlet.com Steps: https://padlet.com/wall/uvkhaz6vnq/wish/25932470 1. Break open cell through chemical or physical method

2. Use phenol and proteinases to remove the proteins

3. Cleave the DNA piece with a restriction enzyme.

4. Cleave the plasmid with the restriction enzyme

5. Ligate using ligase put the recombinant vector into the host cell

6. Plate it

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