The total RNA was extracted from various tissues, including roots, leaves, stem, and flower, at the V4 stages of WT soybeans grown under long-day conditions (16 h light/8 h dark). The qRT-PCR analysis was performed with each GmCAMTA-specific primer (Supplementary Table S2). GmTUBULIN was used as an internal control for normalization.

: analyzed in soybean seedling during abiotic stress by using qRT-PCR

salt 150 mM NaCL

cold 2 C

ABA 100 uM

GmTUBULIN gene control

ABA-induced genes : AtRD29A AtRD28B

proline biosynthesis gene : AtP5CS2

stress-related marker : AtKIN1

AtTUBULIN2 gene control