科學Maker手機光譜儀與imagej光譜分析_0823朴子國中 by 廖酉鎮 https://padlet.com/ml1201/ujpxk70mzyxvd334 en-us 2023-08-19 06:47:34 UTC 2023-08-26 04:08:31 UTC hello@padlet.com 拍下兩張光譜圖後上傳，第一張拍攝校正光源，例如下圖為日光燈汞線光譜，具有汞的譜線可供校正。第二張可隨意針對任何光源或是有顏色物體拍攝 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288672 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288672 下載imagej ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288673 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288673 螢光燈管水銀激發光譜 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288674 https://commons.wikimedia.org/wiki/File:Spectra-Philips_32T8_natural_sunshine_fluorescent_light.svg]]> 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288674 校正波長所在像素位置，以螢光燈管而言，可以用435.8與546兩個波長 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288675 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288675 (5)按 Ctrl+K 繪圖(或Analyze->Plot profile)，找出校正波長所在像素位置，以螢光燈管而言，可以用435.8與546兩個波長 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288676 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288676 (4)選取分析範圍，Ctrl+A全選或手動選取 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288677 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288677 照片常見問題與處理 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288678 https://www.facebook.com/groups/690298274443573/permalink/1468413143298745/]]> 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288678 照片常見問題與處理 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288679 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288679 鏡頭對準光譜儀觀景窗放上去，放上後要避免移動 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288680 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288680 (3)旋轉照片，短波長在左側 image->Transform->rotate 90 Left ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288681 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288681 (2)開啟imagej，打開檔案 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288683 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288683 (1)拍攝校正光源光譜，或是用下圖的汞燈光譜練習校正 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288684 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288684 依需求調整格式與增加資料點 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288685 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288685 選取波長與數值即，插入XY散佈圖 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288686 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288686 利用公式轉換X值為波長，Gray Value為光譜數值 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288688 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288688 將數據複製後貼至Excel ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288689 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288689 選取寬度範圍後，轉成波長與強度的圖(Ctrl+K) ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288690 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288690 開啟相同條件下拍攝的光譜，下圖為同一次拍攝的電腦螢幕光譜 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288691 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288691 阿簡老師部落格-吸收光譜分析 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288692 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288692 阿簡老師的自動分析巨集 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288693 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288693 阿簡老師的教學影片 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288694 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288694 將log檔中轉換方程式資訊複製貼至Excel ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288695 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288695 按下fit，會出現轉換方程式畫面 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288696 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288696 按右鍵->oringinal scale->顯示完整畫面->選取後放大，再到curve fitting視窗輸入第二個波峰X位址與對應波長，兩者間以空一格分開，本例中輸入2588 546，按下fit即完成 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288697 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288697 打開Analyze->tool->curve fitting，刪除原始資料，輸入波峰X位址與對應波長，兩者間以空一格分開，本例中輸入1724 435.8 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288698 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288698 (6)圈選後按+放大選取區以獲得較精確數字 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288699 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288699 四張照片疊起來對照 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288700 只要手機鏡頭有移動，就需要重新拍攝校正光源，並依此狀態拍攝待測光源]]> 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288700 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288701 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288701 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288702 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288702 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288703 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288703 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288704 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288704 以下四張照片，為同一台手機跟光譜儀所拍攝，此四張照片用來校正，得到的校正公式相同嗎? ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288705 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288705 可以多組數據並陳 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288706 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288706 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288707 0.個人使用經驗說明
1.取得imagej
2.光譜儀基本介紹與操作
3.拍攝光譜儀照片
4.光譜儀校正
5.利用校正公式分析待測光譜]]> 2023-08-19 06:47:34 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666288707 本次研習主頁面 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666290487 2023-08-19 06:55:17 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666290487 第二張不知道拍甚麼，可以用這個三原色圖來拍 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666456821 2023-08-19 17:54:58 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666456821 利用校正光源的譜線與照片像素位置的對照，產生轉換公式 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666672513 2023-08-20 13:04:18 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666672513 選取照片中分析範圍，其中必須包含校正光源譜線及後續欲分析的波長範圍，不確定就用全選就好 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666673426 2023-08-20 13:07:13 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666673426 image to stack的好處 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666680933 2023-08-20 13:33:04 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666680933 取得轉換公式，以利後續轉換待測物光譜的波長 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666682448 2023-08-20 13:36:54 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666682448 開啟待測光譜，利用剛剛的公式轉換像素為波長 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666683197 2023-08-20 13:39:31 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666683197 即時光譜 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666693803 https://imagej.nih.gov/ij/plugins/webcam-capture/index.html]]> 2023-08-20 14:06:23 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2666693803 科學Maker粉絲頁 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2667088555 https://www.facebook.com/groups/720651754612879/?ref=share&mibextid=NSMWBT]]> 2023-08-21 04:17:14 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2667088555 科學Maker工具圖鑑 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2667090098 https://ywyang236.blogspot.com/p/maker.html]]> 2023-08-21 04:19:24 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2667090098 取得科學Maker儀器的方式 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2667099727 https://ywyang236.blogspot.com/2021/07/maker_23.html


]]> 2023-08-21 04:31:44 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2667099727 每次使用，需要先拍攝一張校正用光譜照片，再拍攝待測物光譜。 ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2668101581 2023-08-22 01:18:45 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2668101581 不同寬度狹縫的影響為何？ ml1201 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2668108145 2023-08-22 01:23:46 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2668108145 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669570661 2023-08-23 03:17:29 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669570661 H https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669576148 2023-08-23 03:23:19 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669576148 P https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669577346 2023-08-23 03:24:30 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669577346 H https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669577538 2023-08-23 03:24:42 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669577538 P2 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669577752 2023-08-23 03:24:54 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669577752 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669578887 2023-08-23 03:26:02 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669578887 0823 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669579980 2023-08-23 03:27:05 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669579980 shuchen https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669580378 2023-08-23 03:27:32 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669580378 08231 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669580421 2023-08-23 03:27:35 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669580421 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669581601 2023-08-23 03:28:47 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669581601 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669582064 2023-08-23 03:29:15 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669582064 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669582297 2023-08-23 03:29:29 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669582297 Mavis orang https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669583575 2023-08-23 03:30:54 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669583575 dukedidi1 https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669587014 2023-08-23 03:34:54 UTC https://padlet.com/ml1201/ujpxk70mzyxvd334/wish/2669587014