https://padlet.com/ISRStratford/wolbachia_lab Collaborate between groups and classes to fully research the DNA extraction, PCR, and Gel Electrophoresis. Each group will present the details of the Wolbachia lab section that has been designated to your group. en-us 2021-05-14 12:02:23 UTC 2025-07-11 21:35:46 UTC hello@padlet.com https://padlet.net/icons/png/1f9ec.png ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524261812 Cam, Coach Lyon, Julissa, Jacori]]> 2021-05-14 12:08:37 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524261812 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524274925 Lizzie, Jacob, Josiah]]> 2021-05-14 12:14:24 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524274925 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524276287 Ben, Amaje, LaSheka]]> 2021-05-14 12:15:04 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524276287 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524281039 2021-05-14 12:17:20 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524281039 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524285402 Sokera
Tabitha
(Arayna)]]> 2021-05-14 12:19:20 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524285402 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524286986 2021-05-14 12:20:02 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524286986 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524305770 Ryan
Kevin
Lyon
(Destanie)
(Makayah)]]> 2021-05-14 12:27:46 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524305770 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1524322118 2021-05-14 12:33:39 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524322118 jacobdcesario https://padlet.com/ISRStratford/wolbachia_lab/wish/1524354365 In this activity, we are extracting total genomic DNA from our collected insects. Total genomic DNA includes DNA, and the symbiotic bacteria if present in the insect.                                   The first step is Cell Lysis. We began blotting away the ethanol from our insects. Then, we macerated them with Buffer ATL. This breaks open the cell and cell membranes. the problem with this is that it also exposes DNA to proteins in the insect. The enzyme Proteinase K is added to keep the DNA intact and denature proteins. The second step is the elimination of cellular debris. This is a purification process. We put our tubes in the spin column. This spun or tubes around, and cleansed whatever liquid we had inside. This filters everything other than the DNA. Now that it has been filtered, it was washed by two Buffers: AW1 and AW2. Column washing removes residual protein impurities without affecting the DNA.                    The third step is DNA elution. We will complete this step by removing DNA from the filter. This is done by adding the elution buffer AE. Spinning the tube with the DNA in the filter will pull the elution buffer through the matrix, pulling the DNA into the extraction tube. Elution of the bound DNA is typically done with later or a low salt solution like TE Buffer. This is because the removal of salts with the
increase in water molecules unmasks charges and rehydrates the DNA as well as the surface of the silica membrane.]]> 2021-05-14 12:44:02 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1524354365 ISRStratford https://padlet.com/ISRStratford/wolbachia_lab/wish/1525443319 2021-05-14 17:02:16 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1525443319 rasaqo07718 https://padlet.com/ISRStratford/wolbachia_lab/wish/1531931852 Caitlin
Rasaq
Bre]]> 2021-05-17 16:01:48 UTC https://padlet.com/ISRStratford/wolbachia_lab/wish/1531931852