CHM 510 ANALYTICAL SEPARATION METHODS (NOTES) by DR NUR NADIA DZULKIFLI

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GAS CHROMATOGRAPHY

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HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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SUPERCRITICAL FLUID CHROMATOGRAPHY (SFC)

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CAPILLARY ELECTROPHORESIS (CE)

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SAMPLE PREPARATION

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VAN DEEMTER COLOURFUL NOTES

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NORMAL PHASE

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REVERSE PHASE

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CHAPTER 3 HPLC (ADSORPTION CHROMATOGRAPHY)

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SIZE EXCLUSION CHROMATOGRAPHY (SEC) PART 1

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SIZE EXCLUSION CHROMATOGRAPHY (SEC) PART 2

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ION EXCHANGE CHROMATOGRAPHY (IEC) PART 1

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ION EXCHANGE CHROMATOGRAPHY (IEC) PART 2

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ION EXCHANGE CHROMATOGRAPHY (IEC) PART 3

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SEC PART 1

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SEC PART 2

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EXPERIMENT 2: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC): METHOD DEVELOPMENT

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EXPERIMENT 1: GAS CHROMATOGRAPHY (GC): OPTIMIZATION OF FLOW RATE AND COLUMN TEMPERATURE

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EXP 1

EXPERIMENT 3: FATTY ACID DETERMINATION USING GAS CHROMATOGRAPHY (GC)

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ION PAIR CHROMATOGRAPHY (IPC)

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HPLC PART 1

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HPLC PART 3

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SUPERCRITICAL FLUID CHROMATOGRAPHY (SFC) PART 1

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SUPERCRITICAL FLUID CHROMATOGRAPHY (SFC) PART 2

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SUPERCRITICAL FLUID CHROMATOGRAPHY (SFC) PART 3

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BAND BROADENING IN LC

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HPLC PART 2

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CHAPTER 6_PART 1

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CHAPTER 6_PART 2

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CHAPTER 6_PART 3

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CHAPTER 6_PART 4

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CHAPTER 6_PART 5

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REVERSE PHASE_1

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ISOCRATIC & GRADIENT ELUTION

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ION PAIR CHROMATOGRAPHY

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APPLICATION OF HPLC

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ION EXCHANGE CHROMATOGRAPHY (IEC)

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COURSE LEARNING OUTCOMES

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Able to:

1.Explain the fundamental concepts & theories of separation techniques in CE.

2.Sketch, label the schematic diagrams & discuss the function of each component in CE.

3.Identify the strength & limitations of CE technique.

4.Suggest & justify the most suitable & efficient separation technique to be employed for an analysis.

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SIZE EXCLUSION CHROMATOGRAPHY (SEC)

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SIZE EXCLUSION CHROMATOGRAPHY (SEC)_2

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ADSORPTION CHROMATOGRAPHY

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ADSORPTION CONCEPT

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ION EXCHANGE CHROMATOGRAPHY (IEC) PART 2

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DEFINITION OF SUPERCRITICAL FLUID

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COURSE LEARNING OUTCOMES

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Able to:

1.Explain the fundamental concepts & theories of separation techniques in HPLC.

2.Sketch, label the schematic diagrams & discuss the function of each component in HPLC.

3.Identify the strength & limitations of HPLC technique.

4.Suggest & justify the most suitable & efficient separation technique to be employed for an analysis.

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SFC EXTRACTION

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INTRODUCTION OF SFC

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CE_APPLICATION

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CE_EPF AND EOF

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EOF_1

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MICELLAR

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COURSE LEARNING OUTCOMES

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Able to:

1.Explain the fundamental concepts & theories of separation techniques in SFC.

2.Sketch, label the schematic diagrams & discuss the function of each component in SFC.

3.Identify the strength & limitations of SFC technique.

4.Suggest & justify the most suitable & efficient separation technique to be employed for an analysis.

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CHAPTER 6_PART 6

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MARCH - AUGUST 2025

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VAN DEEMTER SHORT NOTE

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SLIDES_CHAPTER 1

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SLIDES_CHAPTER 2 GC

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SLIDES_CHAPTER 3_HPLC_PART 1

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SLIDES_CHAPTER 3_HPLC_PART 2

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SLIDES_CHAPTER 3_HPLC_PART 3

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SLIDES_CHAPTER 3_HPLC_PART 4

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SLIDES_CHAPTER 3_HPLC_PART 5

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SLIDES_CHAPTER 4_PART 1

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SLIDES_CHAPTER 4_PART 2

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SLIDES_CHAPTER 5_PART 1

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SLIDES_CHAPTER 5_PART 2

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SLIDES_CHAPTER 6_PART 1

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SLIDES_CHAPTER 5_PART 2

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SEMESTER OCT 2025 – FEBRUARY 2026 (20254)

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