Module 3_G2 by Laura Bacete https://padlet.com/laurabacete/BI2015_G2_M3 What should be in the report? en-us 2021-10-18 10:18:29 UTC 2021-10-21 09:23:59 UTC hello@padlet.com https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832931038 GFP in general

]]> 2021-10-21 08:57:18 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832931038 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832960482 Hydrophobic interaction chromatography ]]> 2021-10-21 09:14:48 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832960482 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832960835 SDS-PAGE]]> 2021-10-21 09:14:59 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832960835 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832961031 Western blot]]> 2021-10-21 09:15:06 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832961031 Methods for detecting proteins https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832962068 2021-10-21 09:15:44 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832962068 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832962654 Buffers]]> 2021-10-21 09:16:05 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832962654 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832962886 TE buffer (?) instead of water in Lysate ]]> 2021-10-21 09:16:13 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832962886 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963053 Protein purification]]> 2021-10-21 09:16:20 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963053 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963423 Hypothesis]]> 2021-10-21 09:16:33 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963423 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963465 Western blot]]> 2021-10-21 09:16:34 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963465 image of gels https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963567 2021-10-21 09:16:39 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832963567 Recombinant proteins and tags https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832964007 2021-10-21 09:16:55 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832964007 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832964937 Pictures from column chromotography]]> 2021-10-21 09:17:27 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832964937 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832965005 Tagging of proteins]]> 2021-10-21 09:17:29 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832965005 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832965912 Recombinant proteins]]> 2021-10-21 09:17:56 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832965912 Why do we want to produce proteins? Background https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832966033 2021-10-21 09:18:01 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832966033 Host organisms https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832966531 2021-10-21 09:18:21 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832966531 Aim of the experiment, summary etc https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832967639 2021-10-21 09:19:04 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832967639 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832967987 GFP bound to proteins and free gfp in solution]]> 2021-10-21 09:19:17 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832967987 Did we observe other protein bands in the gel? Why? https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832969276 2021-10-21 09:20:02 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832969276 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832969958 Fractions from hic]]> 2021-10-21 09:20:33 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832969958 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832970464 Production and detection ]]> 2021-10-21 09:20:52 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832970464 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832974616 Expections and result ]]> 2021-10-21 09:23:15 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832974616 https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832975909 Possible improvements ]]> 2021-10-21 09:23:59 UTC https://padlet.com/laurabacete/BI2015_G2_M3/wish/1832975909