MOHD 2025 by Mariona Nadal

Muktiple choice questions

2023-11-07 13:56:04 UTC

Group 1: If you want to have a high copy number of plasmids in yeast, which type of plasmid would be the best choice?

A) Yeast replicative plasmid (ARS)
B) Yeast centromeric plasmid (CEN)
C) Yeast episomal plasmid (2μ)
D) Yeast integrative plasmid

Group 2:

Group 3:

Group 4: Which of the following best illustrates how studying yeast MAPK pathways helps scientists understand signaling in multicellular organisms?

A) Yeast MAPK pathways, such as HOG and mating response cascades, use orthologous kinases to those in mammals, revealing MAPK’s regulation of stress and developmental responses.

B) Yeast MAPK pathways are unique and can be compared to mammalian systems but don’t provide enough evidence of how mammalian cells function.

C) Yeast cells rely on MAPK signaling only for glucose metabolism, relating it only to a couple of animal processes.

D) Yeast MAPKs are not phosphorylated and therefore model signaling in higher eukaryotes.

Group 5:

short assay questions:

2023-11-07 13:57:29 UTC

Group 1: Why is S. cerevisiae used as a model organism?

Group 2: Describe the purpose and significance of the Yeast Knockout Collection (YKOC) in biomedical research.

Group 3: How can we study protein interaction using yeast as a model organism?

Group 4:

Group 5:

The yeast two-hybrid (Y2H) system is primarily used to:

2025-11-04 14:22:56 UTC

a) Measure enzyme kinetics in yeast cells
b) Identify protein–protein interactions
c) Detect gene mutations in yeast genomes
d) Quantify mRNA expression levels
e) Study protein degradation via the proteasome

a

krismas84 — 2025-11-04 14:23:05 UTC

4

2025-11-04 14:23:20 UTC

Which of the following best illustrates how studying yeast MAPK pathways helps scientists understand signaling in multicellular organisms?

A) Yeast MAPK pathways, such as HOG and mating response cascades, use orthologous kinases to those in mammals, revealing MAPK’s regulation of stress and developmental responses.

B) Yeast MAPK pathways are unique and can be compared to mammalian systems but don’t provide enough evidence of how mammalian cells function.

C) Yeast cells rely on MAPK signaling only for glucose metabolism, relating it only to a couple of animal processes.

D) Yeast MAPKs are not phosphorylated and therefore model signaling in higher eukaryotes.

What is the main phenotype observed in a hog1Δ yeast mutant?

2025-11-04 14:23:59 UTC

A) Increased cell growth rate

B) Defective adaptation to high osmolarity

C) Overactivation of the TOR pathway

D) Increased mitochondrial respiration

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How does the yeast two-hybrid system work and why is it useful to study protein-protein interactions?

2025-11-04 14:24:07 UTC

Which yeast MAPK is the ortholog of human p38?

a)Pbs2

b)Hog1

c)Ste11

d)Whi5

e)Jnk1

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What is the primary advantage of using yeast artificial chromosomes in research?
a) They improve fermentation speed
b) They behave as normal eukaryotic chromosomes and can store large DNA fragments
c) They increase mutation rates
d) They produce insulin naturally
e) They are used only in bread making

Which of the following statements about Saccharomyces cerevisiae (yeast) is correct?

2025-11-04 14:24:33 UTC

a) Yeast cells only exist in the diploid state under laboratory conditions.
b) The alpha (α) and beta (β) factors refer to different nutrient sources used by yeast.
c) Haploid yeast cells of opposite mating types can fuse to form a diploid cell.
d) Diploid yeast cells immediately die when nutrients are scarce.
e) Yeast cells have a life cycle that takes several days to complete.