Immunofluorescence microscopy by Nagakanni Muthuvenugopal https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08 en-us 2021-06-29 08:03:13 UTC 2024-05-29 13:34:20 UTC hello@padlet.com https://padlet.net/icons/png/1f52c.png fluorescence microscopy nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629785411 an optical microscope that fluorescence instead of reflection, scattering, and absorption.]]> 2021-06-29 08:07:04 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629785411 george strokes nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629788375 first to describe fluorescence]]> 2021-06-29 08:09:46 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629788375 immunofluorescence microscopy nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629790956 assess both localization & endogenous expression levels of POI ]]> 2021-06-29 08:12:23 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629790956 flurophores nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629793179 these are used to visualize the location of bound antibodies]]> 2021-06-29 08:14:15 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629793179 fluorescence nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629796298 Fluorescence is the process where a material absorbs light at a high energy, short wavelength and emits light at a lower energy, usually visible, wavelength.]]> 2021-06-29 08:17:23 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629796298 used on nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629800840 tissue sections, cultured cells and individual cells.]]> 2021-06-29 08:21:38 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629800840 nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629802874 distribution of proteins, glycoproteins, antigen targets]]> 2021-06-29 08:23:38 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629802874 types nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629804171 direct immunofluorescence
indirect immunofluorescence]]> 2021-06-29 08:24:47 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629804171 direct (DIF) nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629806258 single Ab*
conjugated to a fluorescent dye.
eg: ATTO fluorescent conjugates
technique: first introduced by burnham et al., - lesional skin of lupus erythromatosis patients.
]]> 2021-06-29 08:26:49 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629806258 indirect (IFF) nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629808236 use of two antibodies.
promary recog. secondary Ab*  conjugated to a fluorescent dye.
eg: Bovine IgG
technique : first introduced by beutner and jordan. detection of Ab* to the intracellular spaces of squamous epithelia in sera of patients with pemphigus
]]> 2021-06-29 08:28:37 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629808236 disadvantages nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629817850 photo bleaching
auto- fluorescence
non specific fluorescence]]> 2021-06-29 08:37:25 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629817850 photobleaching nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629818972 Photobleaching is the chemical alteration of the indicator dye.
it is unable to fluoresce due to the destruction of covalent or non-covalent bonds due to non-specific binding caused by excitation light.]]> 2021-06-29 08:38:27 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629818972 auto-fluorescence nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629820362 Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength.]]> 2021-06-29 08:39:55 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629820362 MN nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629890036 2021-06-29 09:52:18 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629890036 G & F AF nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629890441 2021-06-29 09:52:48 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629890441 nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629892096 2021-06-29 09:54:40 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629892096 nagakannivenugopal3517 https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629893800 2021-06-29 09:56:25 UTC https://padlet.com/nagakannivenugopal3517/tozttew9l350vc08/wish/1629893800