https://padlet.com/jobrien105/totryqtxt073oetn Jack, Lindsay and Jayda en-us 2020-10-28 12:23:03 UTC 2024-12-18 08:24:51 UTC hello@padlet.com jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/869288696 Procedure:
- Add 5.0 mL of potato extract to Erlenmeyer flask
- Add 5.0 mL of hydrogen peroxide to separate beaker
- Assemble apparatus by filling large beaker and graduated cylinder with water and place graduated cylinder upside down inside large beaker, with tube from Erlenmeyer flask inside graduated cylinder (see image below)
- Record initial volume of air
- Mix two test tubes in Erlenmeyer flask and immediately stopper the tube of the flask
- Record amount of gas collected in graduated cylinder every 10 seconds while agitating flask]]> 2020-10-28 12:28:05 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/869288696 lfeng3 https://padlet.com/jobrien105/totryqtxt073oetn/wish/869289427 Our goals: to see how changing substrate concentration (H2O2) would affect the oxygen production of 5ml potato extract.

Hydrogen peroxide (H2O2) is a chemical compound that can be found in the cell organelles peroxisomes. Peroxisomes break down organic molecules by oxidation to produce H2O2. Because H2O2 can potentially be toxic to the cell (cause disease), peroxisomes also have catalase. The catalase enzyme is important to the cell and our bodies because it prevents oxidative damage by breaking H2O2 down into water and oxygen gas so that it is safe.

Findings: From the data we collected we concluded that the larger the substrate concentration, the more oxygen is produced.]]> 2020-10-28 12:28:20 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/869289427 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875469214 We repeated the same procedure three times, but varied the substrate concentration by changing the amount of hydrogen peroxide each time. For our first experimental procedure, we used 1.0 mL H2O2. Next we used 2.5 mL, and then 4 mL, and continued to record the amount of gas collected in the cylinder each time.]]> 2020-10-30 02:39:58 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875469214 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875481765 2020-10-30 02:48:28 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875481765 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875505318 2020-10-30 03:07:30 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875505318 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875511485 2020-10-30 03:12:21 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875511485 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875515356 2020-10-30 03:15:02 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875515356 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875518436 2020-10-30 03:17:36 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875518436 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875532825 This graph shows the amount of oxygen evolved at 5.0 mL H2O2 over time. The amount of oxygen increases but the rate (2.4 mL/s) begins to slowly decrease until it plateaus at 68 mL, or a gain of 48 mL. ]]> 2020-10-30 03:28:44 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875532825 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875534025 This graph shows the amount of oxygen evolved at 1.0 mL H2O2 over time. The rate of reaction (0.5 mL/s) is far lower than the original test, and it plateaus at 29 mL, or a gain of 15 mL. ]]> 2020-10-30 03:29:51 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875534025 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875535275 This graph shows the amount of oxygen evolved at 2.5 mL H2O2 over time. The rate of reaction (1.6 mL/s) has increased from the previous test as the substrate concentration increases. The graph plateaus as 56 mL, or an increase of 33 mL. ]]> 2020-10-30 03:30:58 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875535275 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875536442 This graph shows the amount of oxygen evolved at 4.0 mL H2O2 over time. The rate of reaction (1.8 mL/s) has increased again and it plateaus at 84 mL, or a gain of 48 mL. ]]> 2020-10-30 03:32:01 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875536442 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/875557216 2020-10-30 03:50:42 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/875557216 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/876067932 2020-10-30 12:13:09 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/876067932 https://padlet.com/jobrien105/totryqtxt073oetn/wish/876252438 The reaction rate of oxygen evolved increases as substrate concentration increases. This is because with more substrate, competition for enzymes with other substrates decreases and the substrate can attach to a higher percentage of available enzymes.]]> 2020-10-30 13:18:56 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/876252438 https://padlet.com/jobrien105/totryqtxt073oetn/wish/876305316 Other factors that could affect enzyme reaction rate are temperature, pH, enzyme concentration, and the presence of other substrates and enzyme inhibitors. Enzyme surroundings and concentrations allow the enzyme to function properly under ideal circumstances. Perfect conditions for specific enzymes vary based on their function and location in the body. ]]> 2020-10-30 13:33:45 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/876305316 jobrien105 https://padlet.com/jobrien105/totryqtxt073oetn/wish/876976773 2020-10-30 16:14:32 UTC https://padlet.com/jobrien105/totryqtxt073oetn/wish/876976773