LIFE227.0 Assessment by Rachel Floyd https://padlet.com/floyd781/t0hfot96g07fr0p9 Ask anything apart from 'how should I analyse the data' en-us 2023-11-27 15:16:55 UTC 2024-11-27 15:41:30 UTC hello@padlet.com https://padlet.net/icons/png/1f929.png https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805418205 Why does the IC50 lane shows a more intensive smear than IC75? Does IC75 even produce a smear and if not why?

]]> 2023-11-28 09:12:34 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805418205 What do you mean by plotting the data as raw OD values? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805667297 2023-11-28 12:52:56 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805667297 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805671822 Do we need to calculate IC50

]]> 2023-11-28 12:56:47 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805671822 Does normalising the data mean to just compare it to the control? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805673574 2023-11-28 12:58:17 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805673574 how many statistical analysis are recommended ? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805830216 2023-11-28 14:35:44 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805830216 Is there a maximum number of figures ? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805864314 2023-11-28 14:56:29 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805864314 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805864749 Do we need to perform a stats test on these results??

]]> 2023-11-28 14:56:46 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805864749 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805865091 What’s the hypothesis?

]]> 2023-11-28 14:56:59 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805865091 What’s the hypothesis? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805865284 2023-11-28 14:57:06 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805865284 What’s the hypothesis? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805865775 2023-11-28 14:57:14 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805865775 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805866904 How do you add error bars to a graph that is showing % viability? Do you have to calculate the standard deviation into a percentage?


]]> 2023-11-28 14:57:45 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805866904 Standard deviation https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805869773 I've calculated the SDs for the data but on google sheets i cant add custom standard deviations

]]> 2023-11-28 14:59:19 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805869773 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805870261 What are we actually investigating

]]> 2023-11-28 14:59:35 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805870261 General question https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805871125 Around how many words should the total method section be?

]]> 2023-11-28 15:00:04 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805871125 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805871449 Do we need to perform statistical analysis on this data too or just immunofluoroesnce?

]]> 2023-11-28 15:00:16 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805871449 Are we plotting the mean for each drug concentration for each lab group or means including all of the groups results https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805871909 2023-11-28 15:00:30 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805871909 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805872665 At what point in the method should the chamber be removed from the slide?

]]> 2023-11-28 15:00:54 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805872665 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805872880 How many graphs do we need

]]> 2023-11-28 15:01:01 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805872880 MLKL clusters https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805873446 What are MLKL Clusters where can i find more about them

What does this protein do

How do we used this to determine the pathway to focus our lab report about ?

]]> 2023-11-28 15:01:21 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805873446 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805874180 Is the mean being calculated for each drug concentration columns or is it for each group’s drug concentrations?

]]> 2023-11-28 15:01:44 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805874180 What is a chamber slide https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805874997 2023-11-28 15:02:14 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805874997 What stats analysis do we need to do https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876060 2023-11-28 15:02:49 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876060 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876172 do we have to reference the manual

]]> 2023-11-28 15:02:54 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876172 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876300 I don’t know how to write a method on the immunofluorescence part when it’s not what we did in the lab. Even with the help material

]]> 2023-11-28 15:02:57 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876300 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876706 What kind of graphs do we need for mtt assay

]]> 2023-11-28 15:03:12 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805876706 Dataset https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805879433 I can see the MTT data and MLKL data on the excel sheet but don’t understand which part of the spreadsheet is the electrophoresis dataset

]]> 2023-11-28 15:04:38 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805879433 OD at 570nm https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805881552 The lab book said purple formazan was quantified at 550-570 nm but the MTT assay sheet says something about OD readings (data prior to normalisation) being read at 570nm. could you please explain this?

]]> 2023-11-28 15:05:51 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805881552 do we add a picture of our gel to the lab report? or just desribe/analyse https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805881704 2023-11-28 15:05:57 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805881704 Method https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805886477 This is probably a silly question as I know methods aren't referenced however are we expected to reference the lab book as this is where we "got" the methods from? Asking because we're expected to reference R if using it is mentioned in the methods :)

]]> 2023-11-28 15:08:42 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805886477 general question https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805886895 why do we only get till monday to do this assignment it seems like a lot to do in such a small amount of time?

]]> 2023-11-28 15:08:57 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805886895 hypothesis https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805897303 i know the FAQ said we don’t have to include a hypothesis if we’ve expanded on the aim of the study but if we do include hypotheses should we stick to one? or could we expand and come up with two for example

]]> 2023-11-28 15:14:15 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805897303 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805900492 when explaining with what concentration we have treated the 2 DNA samples should we use IC50 and IC75 or should we put specific values

]]> 2023-11-28 15:16:17 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805900492 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805904055 What is the protein?

]]> 2023-11-28 15:18:27 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805904055 For the % cell viability, do we keep values over 100 in? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805909094 2023-11-28 15:21:40 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805909094 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805913203 Do we use statistical tests such as correlation coefficient to further our conclusion or not?

]]> 2023-11-28 15:24:03 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805913203 do we have to go into detail on the methods of this? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805933614 2023-11-28 15:36:10 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805933614 Should we include 3 different subheadings for each test in the methods and results session? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805935938 2023-11-28 15:37:36 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805935938 SG58008 - PBS OD is 0.71 and the OD for 0.063mM is 0.718 so the % viability is greater than 100% https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805935955 2023-11-28 15:37:37 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805935955 I don’t understand what the data is that we have been given, what are the numbers and where have they been calculated from https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805937748 2023-11-28 15:38:43 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805937748 Should we include 3 different subheadings for each test in the methods and results session? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805945227 2023-11-28 15:43:28 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805945227 Can we add a picture of the actual electrophoresis gel https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805945843 2023-11-28 15:43:50 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805945843 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805946360 How do they all link together?

]]> 2023-11-28 15:44:11 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805946360 Electrophoresis https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805946817 What figure do we do for this?

]]> 2023-11-28 15:44:30 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805946817 Methods https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805948001 Do we have to do a methods for MTT Assay, im uno fluorescence test, electrophoresis AND our cell count viability under the microscope?

]]> 2023-11-28 15:45:20 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805948001 Would I link The cell count viability experiment we did under the microscope with the MTT assay ? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805952501 2023-11-28 15:48:11 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2805952501 General https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2807874282 What does KH61220 refer to? Is this the derivative of Naphthyridine?

]]> 2023-11-29 19:06:27 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2807874282 General Question https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2808813723 which dataset do we use to calculate IC50?

]]> 2023-11-30 10:08:36 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2808813723 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2809562001 For the OD data plot are we doing the average of each column or finding the average for every student group

]]> 2023-11-30 19:55:41 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2809562001 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2810354202 Do we use the cell viability graph to work out the ic50 or the OD graph?

]]> 2023-12-01 10:37:20 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2810354202 Standard deviation https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2810411530 how do we include standard deviation bars in our graphs when were just plotting the singular mean values against cell viability?


]]> 2023-12-01 11:43:23 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2810411530 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2810990461 What are the units for the drug used

]]> 2023-12-01 22:04:56 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2810990461 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811243356 What are the units for IC50

]]> 2023-12-02 12:01:03 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811243356 Does the electrophoresis NOT tell you if its necrosis or necroptosis? I know it isnt apoptosis https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811293505 2023-12-02 14:08:50 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811293505 General Question https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811333548 Is it okay to use sources that arent scientific articles like NHS websites or articles written by people on websites?

]]> 2023-12-02 15:33:06 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811333548 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811335302 how do i reference the handbook??

]]> 2023-12-02 15:36:58 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811335302 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811383330 Do I need to reference the methods

]]> 2023-12-02 17:27:57 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811383330 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811472420 The Guidance on the canvas does not tell me the volume of IC50 of the drug diluted in growth media and how to make the negative control (untreated cells). How can I figure out them?

]]> 2023-12-02 22:00:26 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811472420 is it ok to reference scientific articles that are from the 2000s or 2010s or are those too old? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811476403 2023-12-02 22:19:53 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811476403 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811493310 Should the whole paper be written in past tense passive voice?

]]> 2023-12-02 23:44:28 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811493310 I’m a bit confused by what IC75 means. Is it the drug concentration where cell viability is 75% or when 75% of cells have died (i.e. cell viability is 25%)? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811511935 2023-12-03 01:30:04 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811511935 in the introduction do we mention the 3 cell death pathways but then state that it was this specific pathway that was discovered? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811681907 2023-12-03 11:36:24 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811681907 https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811800118 Do we need to describe the process of preparing agarose gel in method?

]]> 2023-12-03 15:25:05 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811800118 Whats the point of the equation if we just follow the video to calculate IC50. Can we just use the equation and would x be 50? https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811923912 2023-12-03 18:54:12 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/2811923912 How should I reference the lab book? What should I put for the author, year etc. https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/3236954898 2024-11-27 15:41:29 UTC https://padlet.com/floyd781/t0hfot96g07fr0p9/wish/3236954898