https://padlet.com/amayes81/sy2dln2widxzbyeb en-us 2023-01-29 22:05:18 UTC 2023-01-29 23:19:04 UTC hello@padlet.com amayes81 https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612198 DNA Barcoding is similar to simple sequence DNA. Simple sequences are what is used in the process of fingerprinting. DNA barcoding is fingerprinting for many species, but without the fingers. There are lots of steps to get your final product which includes: sample collection, DNA isolation, PCR, and gel elctrophoresis.]]> 2023-01-29 22:32:30 UTC https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612198 amayes81 https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612346 When collecting a sample, you collect a small piece of tissue and mark the location you recieved the sample. The cell wall (plant cells) or membrane (animal cells) must be broken down first. You can then move onto isolating the DNA from the other cell material that will not be used.]]> 2023-01-29 22:32:59 UTC https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612346 amayes81 https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612469 1) Put cell material into a centrifuge so it will pellet to the bottom of the tube
2) Remove the excess liquid from the top and put it in a separate tube to mix with silica, then incubate so the DNA will bind to the silica
3) Put it in the centrifuge again so the DNA and silica will pellet at the bottom of the tube
4) Remove the excess liquid so you can wash and spin a couple of times to remove impurities. 
5) Add water and incubate so the DNA releases from the silica
6) Put it in the centrifuge so your DNA will be in your excess liquid, while the silica is at the bottom of the tube. Remove the DNA and put it on ice

]]> 2023-01-29 22:33:18 UTC https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612469 amayes81 https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612547 To create multiple copies of the target DNA sequence, the DNA is put into a tube with PCR reagants and placed in a thermocycler. It takes at least 15 cycles to create a suffiecient amount of DNA to use electrophoresis.]]> 2023-01-29 22:33:33 UTC https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612547 amayes81 https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612689 This is what creates the final product of DNA barcoding. It involves using an electrical current to move DNA material through a porous gel in a salt solution. The DNA is put into a stain and loaded into wells of the gel. The negatively charged DNA moves towards the positive end. The smaller pieces of DNA are able to move more quickly through the gel. Once confirmed that there is a PCR product, it can be sequenced. ]]> 2023-01-29 22:34:00 UTC https://padlet.com/amayes81/sy2dln2widxzbyeb/wish/2460612689