LP1 Lab book session by BMB team https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6 Post your answers here en-us 2021-12-15 19:19:10 UTC 2021-12-17 09:59:02 UTC hello@padlet.com 1A anakinchan21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953412416 The steps of the experiment procedures are clear and concise. The writing is not so hard to understand.]]> 2021-12-17 09:33:15 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953412416 Q1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953412615 Good things: 
- a detailed list of the reagents
- simple& understandable procedure

Things could be improved: 
- give concentration& volume for buffer& inhibitors 
- common things e.g. "gloves" not required in the material list 
-  specify how cells can be scraped out of the well e.g. draw a diagram
]]> 2021-12-17 09:33:25 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953412615 question 1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953413376 good things: pipette specificity, page number and date, materials list,
things to improve: more clarity/details (which lysis buffer?)
mark: 42-48 range]]> 2021-12-17 09:34:06 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953413376 Good stuff https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953413772 Well organised layout nice headings uses pg numbers. Got the basics needed for a report. The equipment list is noted.]]> 2021-12-17 09:34:26 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953413772 1b.should be improved https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414354 student should use the exact time instead of a range of time]]> 2021-12-17 09:34:56 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414354 Question 1a https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414386 Clear, concise and appropriate language in the procedure. 

Clear about certain conditions needed in materials and reagents. ]]> 2021-12-17 09:34:58 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414386 question 2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414389 figure 1 and 2 are raw data
no labels on the figures for 1 and 2
mark: 52-58 range]]> 2021-12-17 09:34:58 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414389 Question 1 20xiazimu https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414405 1A. Good things:
- Steps are straightforward, clear, and easy to follow.
- Past tense used. 

1B. The things that can be improved.
- Didn’t specify the brands and details of the reagents.
- Time range used (“10-20 minutes”); should be more specific. 

]]> 2021-12-17 09:34:59 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414405 1A https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414611 - pipettes are detailed
-titles and headings are easy to see
-date is included]]> 2021-12-17 09:35:09 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953414611 1B https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415060 It could be improved by having a statistical analysis, adding more detail so its more easily replicable and by providing collaborations ]]> 2021-12-17 09:35:31 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415060 1B https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415444 -concentrations and detail about lysis buffers and inhibitors
-quantifying how many drops of lysis buffer added

]]> 2021-12-17 09:35:51 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415444 1C https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415459 Mark: 65]]> 2021-12-17 09:35:51 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415459 Q2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415505 2A raw: figure 1 and 2]]> 2021-12-17 09:35:54 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415505 Improve https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415683 Paddedrocedure very vague specific enough.  No quantirive values used or timing specifics]]> 2021-12-17 09:36:04 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415683 2A https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415972 figure 1 and 2]]> 2021-12-17 09:36:17 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953415972 question 3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416161 dont really identify where the problem could have come from, or how to fix the problem they encountered.]]> 2021-12-17 09:36:26 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416161 Q2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416228 Raw data: Figure 1& 2

Things to be improved:
- label columns& rows in Fig 1
- annotate the western blot e.g. add a ladder, label what are added in each lane
- add error bars to Figure 3]]> 2021-12-17 09:36:30 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416228 Q1. https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416634 Good things
- Clear steps
- Included the date and title
- Page number included
- Written in past tense

Improve
- More specific with materials (e.g which inhibitor?, concentration?, how many drops?)
- More specific timing not the range

]]> 2021-12-17 09:36:49 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416634 2B https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416894 -labelling and annotating all figures and axis
-adding a key to explain the colours in the excel]]> 2021-12-17 09:37:00 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953416894 Example 1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417073 Mark: 60%

Some materials and reagents are not given the volumes used, and some descriptions like "protease inhibitors" are not specific enough (what are the names of the inhibitors exactly?) 

Procedure is concise and easy to follow, but occasionally the timings are vague such as "chilled on ice for 10-20 mins" (gives a range instead of exact time).]]> 2021-12-17 09:37:09 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417073 Question 1 tszyeung21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417096 1a) Major information included: dates, title, materials etc.

1b) More detail is needed for the procedures and materials such as amount of ice etc.

1c) 58]]> 2021-12-17 09:37:11 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417096 Question 1 natalieng21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417133 A) Orgaised well with headings, date and page number. Procedure and timings are shown and written clearly.
B) It is missing some specific details; saying "a few drops" is too vague.]]> 2021-12-17 09:37:12 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417133 Question 1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417620 Good things 
Specific times mentioned 
They referred back to other page numbers 
Did mention some specific measurements 
Detailed equipment list 

Bad things 
Gloves don’t need to be mentioned 
Be more specific about measurements of buffer and inhibitors and how many drops 
Describe in more details their actions performed
 


]]> 2021-12-17 09:37:36 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417620 Procedure sunainasamirborkar21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417696 A) - Good amount of detail included to make it easy to follow

B) - Could improve on including the concentrations of the reagents used in the procedure
- Materials commonly available in the lab like gloves do not need to be mentioned]]> 2021-12-17 09:37:40 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417696 2A https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417881 Figure 1 and 2 ]]> 2021-12-17 09:37:48 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417881 1a. https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417899 -Good page organisation and formatting
-Page number and date
-Legible handwriting

]]> 2021-12-17 09:37:49 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953417899 Question 2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953418112 A.    Figures 1 and 2 are raw data
B.    Improvements - Headers and titles for table of raw data. Axis and sample labels for western blot. Add error bars for the graph. Formatting isn’t clear – doesn’t explain why different colours have been used. 
]]> 2021-12-17 09:37:59 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953418112 1a rp621 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953418194 Past tense
fair amount of detail
easy to follow 

]]> 2021-12-17 09:38:03 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953418194 Good points: https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953418583 The lab book is dated
Pages numbered
Timings measured

Bad points:
Measurements are given
Some general information put down
Branding needed
Specific timings not given]]> 2021-12-17 09:38:21 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953418583 Q2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419075 - figures 1 & 2 are raw data
- the raw data should be labelled with information regarding the trials and substances used, and the band weight in the western blot.
- we would give a mark of ~50%]]> 2021-12-17 09:38:41 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419075 Question 1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419425 Neat and clearly laid out
Should specify the concentration of lysis buffer
Give time periods
Specify how many drops rather than few
Would get 65


]]> 2021-12-17 09:38:58 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419425 Question 1: Protocols https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419616 1A) Good
  • Clearly laid out in a list, easy to read, titles/subtitles stand out
  • Specific pipette tips
  • Include page numbers
  • Each step clearly described

1B) Bad
  • Not specific times, ranges only
  • Didn’t include specific names of reagents
  • More specific details about each step
  • Included obvious things on procedure e.g. gloves

63
]]> 2021-12-17 09:39:06 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419616 2B https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419731 They could have provided some more context and explanation behind each Figure, and maybe produced other different ways of displaying the data, as well as adding a scale and labels for the axes for Figure 2]]> 2021-12-17 09:39:11 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953419731 Question 2: Data Presentation https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420155 2A) Figure 1 and 2 are raw data

2B) Improved:
  • Annotation of column of rows - add units
  • Annotation Western Blot w/ sizes, antibody used, etc. + ladder
  • Add error bars to the graph

53
]]> 2021-12-17 09:39:30 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420155 Q2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420166 2B: Rows and columns in Figure 1 should be labelled
Ladder should be included in Western blot in Figure 2
Label name of sample in Western blot]]> 2021-12-17 09:39:31 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420166 Question 2 tszyeung21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420239 2A) Figure 1 and 2

2B) Labelling is missing for both raw data, unclear and difficult to interpret. Ladder for figure 2 is missing. Error bars are missing for the processed data figure 3. 

2C) 45]]> 2021-12-17 09:39:32 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420239 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420388 1A
date
page number


1B
More specific in materials 
Volumes of buffer are not shown
no attribution of collaboration


2A. Figure 1 and 3
2B. No axis on the Western Blot (no labels)

3. Only mentioned that it has been contaminated without suggesting a possible cause. ]]> 2021-12-17 09:39:40 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420388 3A https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420537 -mention what they think went wrong and how they can improve this to prevent contamination again]]> 2021-12-17 09:39:47 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420537 Question 3: Troubleshooting https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420548 3A) 0-38
They only talked about what went wrong but not why and how to fix it.
  • Didn’t address problem and where it came from
  • Didn’t attempt to suggest as to how to solve the problem.
To Improve:
  • Suggest why they think they went wrong
  • Suggest how they would improve/fix the problem

35
]]> 2021-12-17 09:39:48 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420548 1b. https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420623 -No quantities listed in the materials and reagents
-Timings not given
-Gloves don't need to be listed in the equipment list

]]> 2021-12-17 09:39:51 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953420623 1b rp621 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421459 add concentrations and volumes
no time ranges, only specific times
don't need to mention gloves


]]> 2021-12-17 09:40:28 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421459 Example 2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421525 Mark: 42-48%

Raw data (Figure 1 and 2) lacking annotations so unclear to the reader what the data is showing. 


]]> 2021-12-17 09:40:31 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421525 2a https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421695 Figures 1 & 2 are raw data]]> 2021-12-17 09:40:40 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421695 2C https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421703 62]]> 2021-12-17 09:40:41 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421703 Question 3 20xiazimu https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421713 - Suggest which specific step may have went wrong.
- Suggest possible solutions to their problem: e.g. avoid contamination: no double dipping, make sure equipment are disinfected before and after use.

]]> 2021-12-17 09:40:41 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421713 Q3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421952 - they have clearly identified the issues
- they should explain possible causes of error as well as improvements for future experiments ]]> 2021-12-17 09:40:53 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953421952 Q1 marlinli21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953422147 1B:
don't need to include common tools(gloves)
quantity of inhibitor added should be specific
time for cooling should be specific
for the ingredient part, the amount of reagent should be included


]]> 2021-12-17 09:41:02 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953422147 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953422169 2a. Figures 1 and 2
2b. Axis labels missing for figure 1 and 2]]> 2021-12-17 09:41:03 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953422169 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953422704 Q1: Unnecessary detail such as gloves in materials section, in protocol section there was no specification of measurements which means the experiment is not reproducible.  42-48 %

Q2: Figure 1 and 3 were good and well-labelled. Figure 2 was not labelled at all so we did not know which protein was run and what are the size of the fragments. 52-58%

Q3. Identified there was a problem but did not identify its cause or how to improve. Troubleshooting failed. 42-48%]]> 2021-12-17 09:41:28 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953422704 2b https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423279 -add a key to explain the different colours in the table in Figure 1
-add a title to the table

]]> 2021-12-17 09:41:58 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423279 Q1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423378 A
- Clear procedure of the experiment. 
- Listed all steps and reagents
- well organised
- written in past tense

B
- hard to follow
- when and how vacuum aspirator is used is not mentioned
- specific time length for chiliing tubes is not mentioned(10-20 mins)
- no detailed explanation of each step
- the amount of buffer and each inhibitor are not mentioned]]> 2021-12-17 09:42:03 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423378 Question 2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423444 Figure 1 and 2 have good raw data but figure 3 is missing labels
50]]> 2021-12-17 09:42:06 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423444 Question 2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423480 2A. Raw data shown in figures 1 and 2
2B. Improvements: Labelling the table and the western blot as it is difficult to understand what is being shown. There is also no ladder for the western blot and controls are not indicated. Figure 3 could be improved by adding error bars. ]]> 2021-12-17 09:42:07 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423480 3A https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423513 They should also include solutions to their problems aside from just stating what went wrong. ]]> 2021-12-17 09:42:08 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423513 Question 2 natalieng21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423582 A) Figures 1 and 2 are raw data. Figure 3 is not raw data.
B) For figure 1, there is no information on what is in each well. For figure 2, there is no ladder so the base pair lengths cannot be known, and no description of what was in the sample and which antibody was used. Because of these reasons the data is of very little use as it cannot be understood.]]> 2021-12-17 09:42:11 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423582 Question 1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423689 -       equipment was stated with good presicion (ie specific micropipettes, microcentrifuge tubes and etc).
-       Sequence of steps were accurate and clear.
-past-tense used
 
-       Unnecessary stuff like gloves were stated in the materials&reagents – they exist in every lab anyways.
-       Few drops in procedure not detailed enough.
-       Inhibitors not specified in the procedure (may be okay since they were included in the procedure: add both inhibitors would be better)
-       Insufficient details regarding centrifugation.
-       Time required for cooling is stated as a range. the student  should record the exact amount of tine
 
52-58 %
]]> 2021-12-17 09:42:16 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423689 2A anakinchan21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423947 Figure 1 and 2]]> 2021-12-17 09:42:31 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953423947 Q3 ts1221 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953424262 They could have identified and explained what error in their protocol led to contamination. Furthermore, they could have suggested ways to improve their experiment in avoiding contamination 
42-48%]]> 2021-12-17 09:42:45 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953424262 Question 3 tszyeung21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425033 The issues were identified (contaminated yoghurt). However, it does not consider likely causes for yoghurts being contaminated and steps moving forwards. As well as the impact of it on the data obtained and evaluation of its occurrence.]]> 2021-12-17 09:43:15 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425033 Q3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425425 - Did not list a cause
- Did not suggest how to overcome it
- Did not describe how contamination was identified]]> 2021-12-17 09:43:34 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425425 Troubleshootin: https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425717 It should have been suggested what the cause of the problem was rather than simply stating the problem.
Moreover they should have mentioned likely improvements they could make for a repeat.]]> 2021-12-17 09:43:50 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425717 Question 2 20xiazimu https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425757 2A. Figure 1 & 2 are raw data.

2B. 
- Significant number in Figure 1 should be consistent.
- Label bars with their values on top. 
- Annotate the Western blot

]]> 2021-12-17 09:43:52 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953425757 2B anakinchan21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426186 I have no idea what the number in figure 1 represent, and have no idea what the image in figure is about.
Clear labelling and addition of axis should be considered to make a easy-to-understand figure]]> 2021-12-17 09:44:13 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426186 Question 1 marianacabaca21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426242 A) 
- format of the document
- headings 
- page number
- all materials are written
- writing is clear

B) 
- missing specific amounts and measurements
- gloves is not necessary in materials list
- inhibitors not specified
- not formatted in clear steps but in a vague past tense account of events]]> 2021-12-17 09:44:16 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426242 Question 1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426578 WWW:
The procedure is easy to follow
Timings are mentioned in the procedure 
Equipment list is detailed 

EBI:
No mention of controls
Equipment, such as gloves, which do not directly correlate to the method can be left out.

]]> 2021-12-17 09:44:32 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426578 2a rp621 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426849  Figure 1 & 2 are raw data.]]> 2021-12-17 09:44:47 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953426849 Question 1 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953427442 1a. Included timings which is useful.
Procedure is written in a concise manner.
1b. Vague language - "protease inhibitors" mentioned but what type of inhibitor is not mentioned. Also time range 10-20 minutes used might make experiment hard to reproduce.]]> 2021-12-17 09:45:13 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953427442 Q1: a_121 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953427466 - clear and concise 
- logical and accurate steps
- good layout
- use of page numbers

- not specific enough "a few drops" and "10-20 mins"
- no need to mention basic necessities (gloves)]]> 2021-12-17 09:45:14 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953427466 Question 3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953428112 Doesn't suggest what or why the contamination occured
Haven't suggested a way of solving the issue

]]> 2021-12-17 09:45:50 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953428112 3a https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953428393 They could suggest why the yoghurt had been contaminated rather than just saying the problem. They could also suggest what they could do next time to avoid having the same problem.]]> 2021-12-17 09:46:04 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953428393 Example 3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953428964 Mark: 42-48%

Student did not give reasons for why the yoghurt might have been contaminated, and therefore no steps to take next time to prevent the mistake. ]]> 2021-12-17 09:46:34 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953428964 Question 2 marianacabaca21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953429142 A)
- Figures 1 and 2

B) 
Figure 1:
- units are absent
- no legend explaining colour differences in numbers

Figure 2:
- annotations not present (weight, size of bands, column title, row titles)
- the picture should've been taken from the front (not clear enough from this angle)]]> 2021-12-17 09:46:44 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953429142 Question 1 monicajinyang21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953429319 1A.
- references to the manual rather than a copy-paste
- written in past tense
- clear structure and listing of steps and reagents/materials

1B.
- no measurements are provided in the procedure
- generic names are given in the materials/reagents e.g. protease inhibitor rather than the name of the inhibitor specifically used for this
- in step 3 of the procedure, the student fails to specify the time they employed and instead gives the time frame given in the manual]]> 2021-12-17 09:46:51 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953429319 Question 3 natalieng21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953429841 A) The student has not written down any of their possible ideas on why the procedure has gone wrong. They have only state what has happened, they have not actually performed a troubleshooting of the problem.This is not helpful as they would not know how to avoid this error occurring in future experiments.]]> 2021-12-17 09:47:16 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953429841 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430029 2A - Figure 1 and 2
2B - The legend could be written below the figure rather than to the side, data is not labelled for the Western Blot, the table is not labeled. ]]> 2021-12-17 09:47:26 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430029 Q2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430483 A
- Figure 1 & 2

B
- rows and columns are not labelled (Fig. 1)
- no annotation of the western blot and no ladder(Fig. 2)
- no positive controls(Fig. 2)
- no error bars(Fig. 3)]]> 2021-12-17 09:47:48 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430483 Question 3 marianacabaca21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430572 How to improve:
- explain in more depth the smell (more descriptions) - How do you know it was contaminated? 
- explore why it might have been contaminated
- suggest solutions on how to avoid this in the future]]> 2021-12-17 09:47:53 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430572 Question 2 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430674 2a. Figure 1 and Figure 2.
2b. No labelling of base pair lengths next to bands on figure 2. No error bars or standard deviation.]]> 2021-12-17 09:47:58 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953430674 2b marlinli21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953431120 In the excel table, the title of each column should be labeled
make sure that carrying the same number of replications under each condition]]> 2021-12-17 09:48:19 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953431120 Question 2 monicajinyang21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953432408 2A.
- Figure 1
- Figure 2

2B.
- Figure 1 lacks any labelling of what do the values of the cell mean
- Figure 2 is missing labelling of the lane, antibody employed and molecular weight of the band
- Figure 3, given that it is processed data, lacks standard error

]]> 2021-12-17 09:49:27 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953432408 Question 3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953436990 Steps to solve errors have not been identified]]> 2021-12-17 09:52:35 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953436990 Question 3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953437186 They don't explain how they have been contaminated, or in what way the yogurt is contaminated, which is very vague.
They do not discuss ways in which contamination could be avoided.]]> 2021-12-17 09:52:45 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953437186 3 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953438836 suggest which step cause the contamination and how to avoid it]]> 2021-12-17 09:53:57 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953438836 Question 3 monicajinyang21 https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953443905 3A. 
the student fails to discuss steps to overcome some pitfalls encountered. as discussed in the group, we suggested the student to:
- suggest ways, new methodologies, in which they could identify whether the variation of soya milk concentrations affects the outcome yoghurt
- consider whether other factors could have made the yoghurt contaminated
- go through the lab book and determine if human error could have lead to yoghurt contamination]]> 2021-12-17 09:58:19 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953443905 3A https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953444695 -No solutions were provided to overcome the issue
-lack of details in how the procedure went wrong
-no evidence on how the problem came about
-should be written in third person point of view]]> 2021-12-17 09:58:57 UTC https://padlet.com/MedicalBiosciences/sy0e2vu6h3u7ehj6/wish/1953444695