https://padlet.com/tierrageorge01/swj0hmgqo5xj By: Tierra R. George en-us 2016-09-21 11:36:23 UTC 2023-01-30 23:51:18 UTC hello@padlet.com tierrageorge01 https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125353635 The purpose of this experiment is to prove that oxygen and water are the products of a liver enzyme reaction and to see what happens when the enzymes are heated over their optimal temperature.]]> 2016-09-21 11:44:42 UTC https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125353635 tierrageorge01 https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125355605 In this Liver Enzyme experiment, we will be using test tubes and pieces of liver to observe how the enzymes in a liver react to hydrogen peroxide. In this experiment, the liver represents the enzyme, the peroxide is the substrate, and the oxygen and the water are the products. Each test tube reaction helps us gain more information about the way enzymes work and the effect of temperature. ]]> 2016-09-21 11:54:27 UTC https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125355605 tierrageorge01 https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125356623 - Five test tubes
- Test Tube holder
- 3 pieces of regular liver
- 1 piece of boiled liver
- Hydrogen Peroxide
- Matches/ Wooden Splint
- Liquid Water 
- Prongs/Something to pick up the liver with (optional)]]> 2016-09-21 11:59:32 UTC https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125356623 tierrageorge01 https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125358025 Step 1: Find a clean space to work on
Step 2: Take out all of your materials
Step 3: Add about 2 centimeters of water to Test Tube 1, then add a piece of liver and push it to the bottom. 
Step 4: Observe what happens
Step 5: Fill up the 2nd Test Tube with 2 centimeters of peroxide.
Step 6:  Add a piece of liver to Test Tube 2 and push it to the bottom. 
Step 7: Observe the reaction (Bubbles up).
Step 8: Prove that oxygen and water are the products.
Step 9: Fill up the third Test Tube with 2 cm of peroxide.
Step 10: Light your wooden splint on fire and let it burn for awhile. When its glowing red, slowly put this down into the tube.
Step 11: Observe the reaction (Nothing happens because peroxide is not flammable).
Step 12: Fill the 4th test tube with 2 cm of peroxide, add a piece of liver, push it down, and wait until it bubbles up.
Step 13: Light your wooden splint. After letting it burn, when it glows red, touch the tip of the splint to the bubbles rising out of the test tube. Repeat this process. 
Step 14: Observe the reaction (It catches on fire).
Step 15: Put 2 centimeters of water into the last Test Tube (5), then place a piece of BOILED liver into the tube and push it down.
Step 16: Drop some peroxide into the test tube and observe how the enzymes do not react the same way with the peroxide. They do not bubble up, they are "dormant".]]> 2016-09-21 12:06:15 UTC https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125358025 tierrageorge01 https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125358248 1) What are enzymes? What are their roles in chemical reactions?
      R= Enzymes are specialized proteins that speed up the process of a chemical reaction. 
2) Describe what happened to test tube 2 when hydrogen peroxide was added to the liver. What gas was produced? What liquid was let behind?
      R= When hydrogen peroxide was added to the liver in test tube 2, it began to fizz up and a column of bubbles rose up and out of the test tube. The gas produced was oxygen and the hydrogen peroxide was left behind. 
3) What effect does the red hot wood have on test tube 4? Why is the flame being produced?
      R= For test tube 4, I am proving that the products, oxygen and water are being given off. After the hydrogen peroxide and the liver react and bubble up, the hot stick dies out because the reaction is also giving off water, so it cools off, but on the second try, the contact with the red hot wood causes it to catch on fire and for the "bubbles" to go down a little. The flame is being produced because pure oxygen in the presence of a spark, creates fire. 
4) What effect does temperature have on the enzymes?
R= If the enzymes in the liver are heated over their optimal temperature, it loses its structure and the bonds break, meaning, it loses its shape and the substrate can longer fit. For instance, in this experiment, since the liver was already boiled, it was over heated, so the peroxide (substrate) did not react with the enzyme the same way.

]]> 2016-09-21 12:06:54 UTC https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125358248 tierrageorge01 https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125573586 2016-09-21 22:36:38 UTC https://padlet.com/tierrageorge01/swj0hmgqo5xj/wish/125573586