https://padlet.com/phulukdaree/su67dvemcf28 To keep an online database of data generated and analysis done during my absence en-us 2017-04-13 10:54:23 UTC 2023-07-08 23:25:16 UTC hello@padlet.com phulukdaree https://padlet.com/phulukdaree/su67dvemcf28/wish/166225187   ]]> 2017-04-13 10:54:23 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/166225187 phulukdaree https://padlet.com/phulukdaree/su67dvemcf28/wish/166225193 AP: Are there any concepts in our plan of action that were unclear? 

AP: Mary-anne, Please attend the MSc Committee meeting with Dr Soma. Send her an email indicating that you will need to since I am on leave. I will advise if anything changes.

AP: Sandra, Prof AJ will attend your PhD and ethics meeting with you.



]]> 2017-04-13 10:54:23 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/166225193 phulukdaree https://padlet.com/phulukdaree/su67dvemcf28/wish/166225348 2017-04-13 10:57:14 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/166225348 phulukdaree https://padlet.com/phulukdaree/su67dvemcf28/wish/166225432 12/04/17 - Made up CCM with EMEM and thawed cells


]]> 2017-04-13 10:58:39 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/166225432 phulukdaree https://padlet.com/phulukdaree/su67dvemcf28/wish/166225460 13/04/17 - Discussed MTT and CV first repeat results. Did repeat of MTT and CV assays using low range of eicosanol. Changed media in 2 x T75. Could not split cos cells were stressed.

]]> 2017-04-13 10:59:01 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/166225460 phulukdaree https://padlet.com/phulukdaree/su67dvemcf28/wish/166226146 2017-04-13 11:11:31 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/166226146 phulukdaree https://padlet.com/phulukdaree/su67dvemcf28/wish/167234465 1 June 2017

Thank you for the update.

Can we please update a representative melt curve (select the controls) for each gene for the MDAs and then for the HepG2s as well?

Thank you.]]> 2017-04-20 12:18:12 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/167234465 https://padlet.com/phulukdaree/su67dvemcf28/wish/167272927 Split the cells and plated in 2 flasks]]> 2017-04-20 14:27:35 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/167272927 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/170482178 24/04/17-26/04/17
First MTT and CV repeat

08/05/17-10/05/17
Second MTT and CV repeat

15/05/17-19/05/17
Third MTT and CV repeat


]]> 2017-05-08 12:05:30 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/170482178 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/172775431 2017-05-19 11:23:49 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/172775431 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/172775603 2017-05-19 11:25:15 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/172775603 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/172775667 2017-05-19 11:25:51 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/172775667 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/172775691 2017-05-19 11:26:07 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/172775691 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/172775809 2017-05-19 11:27:00 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/172775809 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/172775837 2017-05-19 11:27:14 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/172775837 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/172776034 Hi Alisa, Please see attached the 3 repeats for CV and MTT. I am busy with the 4th repeat of both CV and MTT . Thanks 
Regards]]> 2017-05-19 11:28:55 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/172776034 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/173117334 2017-05-22 11:48:10 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/173117334 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/173117593 2017-05-22 11:49:45 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/173117593 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/173118235 2017-05-22 11:52:53 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/173118235 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/173118379 2017-05-22 11:53:47 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/173118379 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/173118483 2017-05-22 11:54:32 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/173118483 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/173118796 2017-05-22 11:56:26 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/173118796 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/173119018 19/05/17-22/05/17
HepG2 RNA isolation and quantification 

23/05/17
Standardize RNA and make cDNA

24/05/17
qPCR:
FASN and HMGCR

25/05/17
qPCR:
LC3 and ACLY]]> 2017-05-22 11:57:32 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/173119018 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/174569052 qPCR analysis: ACLY]]> 2017-05-31 07:55:20 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174569052 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/174569355 qPCR analysis: FASN]]> 2017-05-31 07:57:37 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174569355 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/174569448 qPCR analysis: HMGCR]]> 2017-05-31 07:58:17 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174569448 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/174569507 qPCR analysis: LC3]]> 2017-05-31 07:58:46 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174569507 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/174589736 2017-05-31 10:26:43 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174589736 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/174589971 2017-05-31 10:28:28 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174589971 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/174590189 2017-05-31 10:30:14 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174590189 sndanielles https://padlet.com/phulukdaree/su67dvemcf28/wish/174590242 2017-05-31 10:30:44 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/174590242 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175526569 2017-06-07 09:23:07 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175526569 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175526572 2017-06-07 09:23:08 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175526572 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175526755 2017-06-07 09:24:31 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175526755 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175526895 2017-06-07 09:25:45 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175526895 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175527056 2017-06-07 09:26:58 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175527056 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175527379 HepG2 ACLY Mo Melt Curve]]> 2017-06-07 09:29:37 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175527379 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175527762 HepG2 LC3 Mo Melt curve]]> 2017-06-07 09:32:45 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175527762 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175527802 HepG2 HMCR Mo melt Curve]]> 2017-06-07 09:33:09 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175527802 maphasha97 https://padlet.com/phulukdaree/su67dvemcf28/wish/175528006 HepG2 FASN Mo Melt Curve]]> 2017-06-07 09:34:52 UTC https://padlet.com/phulukdaree/su67dvemcf28/wish/175528006