https://padlet.com/rciprian1/sie4he9fc81s Oral Microbiomes Unknowns ( 23A and 23B) en-us 2020-03-04 20:42:54 UTC 2026-04-10 23:09:29 UTC hello@padlet.com rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454683747 These organisms are Gram negative bacteria composed of pleomorphic cells which are bounded by a triple-layered membrane and no cell wall.  The appearance of their colonies demonstrate a "fried egg" appearance with a small central nipple which is common in Mycoplasma colonies. It is also considered to be a facultative anarobe although the growth is better under anarobic conditions. Its preferance of habitat for maximal growth is in the human orpharynx at a temperature of 36 degree celsius and pH 7. [Mycoplasma Buccale]]]> 2020-03-04 21:12:54 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454683747 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454704089 Mycoplasma usually colonizes the throat, it is the bacterial biofilm, or tartar found in the oral cavity. Also it is one of the common causes of acute respiratory infections. Mycoplasma can be separated from root canal infections, gingivitis, and periodontitis clinical specimens, the distribution and pathogenicity in the oral cavity are still unclear. It is can also initiate and progress oral cancer in humans.]]> 2020-03-04 21:54:21 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454704089 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454704118 A pathogenic bacterium, the most frequently found cell morphology is short bacillus, but long bacilli are sometimes observed. They are classified as black pigmented bacteria due to its formation of shiny and smooth colonies which appear either a grey, light brown, or black color on blood agar plates. The cells are nonsporulating, nonmotile, anaerobes, and stain gram-negative. It is commonly isolated from dental cyst, and are a major component of biofilm formation. P. intermediate use steroid hormones as growth factors. They can be found in high concentration in pregnant women and women with bacterial vaginosis [Prevotella Intermedia]]]> 2020-03-04 21:54:26 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454704118 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454704144 Many successful studies have demonstrated the malignant potential of Mycoplasma in prostate cancer, myeloid cell lines, and mouse embryo cells, but there was no substantial evidence linking Mycoplasma to oral cancer. At that time (2005), there was a lack of in-vivo studies illustrating the pathogenesis of Mycoplasma in oral potentially malignant disorders. In vivo studies using animal models like the Syrian hamster cheek pouch may aid us in understanding the molecular pathway of Mycoplasma induced oral carcinogenesis, which in turn will provide us with vital diagnostic markers and potential therapeutic targets. ]]> 2020-03-04 21:54:30 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454704144 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454704182
Domain: | Bacteria
Phylum: | Firmicutes
Class: | Mollicutes
Order: | Mycoplasmatales
Family: | Mycoplasmataceae
Genus: | Mycoplasma
Species: | M.  Buccale

]]> 2020-03-04 21:54:35 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454704182 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454704225 In the picture below (phylogenetic tree), one can see that the most recent common ancestors are Prevotella Bivia and Prevotela Buccalis. The most recent addition to the phylogenetic tree is Prevotella Buccae. Also, the oldest ancestor is Leptotricia Buccalis. ]]> 2020-03-04 21:54:42 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454704225 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454704246  Prevotella intermedia has serious implications in the context of medical pathology for humans, It is one of the leading causes of periodontal disease. It is transmissible by saliva and kissing. it can cause subgingival plaque, tissue attachment loss, and probing depth beyond standard ranges. One of the main reasons why P. intermedia has a virulent impact on the connective tissue of the mouth, its because it can form biofilm which then turn into dental plaque; If the plaque is persistant it could leave the mouth vulnerable to infections.
[Prevotella Intermedia]]]> 2020-03-04 21:54:46 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454704246 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/454704296 Prevotella intermedia ZT belongs to a genus marked with highly dynamic genomes. The specific genes of Prevotella intermedia indicate that adhesion, competing with surrounding microbes and horizontal gene transfer are the main drive of the evolution of Prevotella intermedia.]]> 2020-03-04 21:54:52 UTC https://padlet.com/rciprian1/sie4he9fc81s/wish/454704296 rciprian1 https://padlet.com/rciprian1/sie4he9fc81s/wish/459567760 There are different ways to determine a species of bacteria from the genome that it carries without knowing its identity because some genes are specific to the species. One can use a tool that gives access to multiple sequences of RNA/DNA or even protein that can match the sequence with a percentage of similarity to different species. BLAST is this tool and it allows us to  use 16s ribosome to reconstruct phylogeny because it has a slow rate of evolution. Thus it allow us to get the specific species from its nucleotide sequence and eliminate all the species related to it. Also using phylogenetic trees allow me to understand and put into simple terms the ancestral relationship of different organisms in a simplified form and more easy to analyze. My two unknowns were not hard to find information about them once I knew their identity; I was able to find that they were both part of oral disease, they are gram negative bacteria and they do not provide any benefits to the body but dangerous disease. There are a lot research towards them and new findings keep appearing with recent research that will help humans tolerate their dangerous life style in our body. Finally, the TED talk by Dr. Jonathan Elsen was interesting to watch because it showed me that microbes are not all harmful to us humans but they provide benefits that our body alone cannot do. There are more cells from microorganisms than our own cells and the best understanding we can get from their job in our body is by looking at its genome. This will allows us to identify the species as a friend or foe.
Amazing Findings:
Kayla Smith
Nathanael Boone


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