Gram Staining by Carol Nicosia https://padlet.com/cnicosia/s7psbh0e0s9s All information gathered on Gram Staining en-us 2017-03-01 19:19:24 UTC 2023-05-30 10:40:15 UTC hello@padlet.com What is Gram Staining https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158043910 Gram staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safanin.]]> 2017-03-06 15:03:12 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158043910 Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158043981 2017-03-06 15:03:24 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158043981 Gram Staining https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044023 2017-03-06 15:03:30 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044023 Gram staining https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044049  The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.
http://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html]]> 2017-03-06 15:03:34 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044049 The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044101 2017-03-06 15:03:42 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044101 The Gram stain is the most important staining procedure in microbiology. It is used to differentiate between gram positive organisms and gram negative organisms. Hence, it is a differential stain. ... Gram negative and gram positive organisms are distinguished from each other by differences in their cell walls. https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044210 2017-03-06 15:03:58 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044210 Gram staining method, an important procedure in Microbiology, was developed by Danish physician Hans Christian Gram in 1884, which is where the name of the technique came from. https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044388 2017-03-06 15:04:23 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044388 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044437 Gram staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safanin.]]> 2017-03-06 15:04:30 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044437 has a thick mesh like cell wall made of peptidoglycan https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044450 2017-03-06 15:04:32 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044450 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044455 2017-03-06 15:04:32 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044455 In gram staining, gram positive bacteria are stained purple whereas gram negative bacteria are stained red. https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044468 2017-03-06 15:04:34 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044468 Things needed to do the staining https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044474
  • Crystal violet (primary stain)
  • Iodine solution/Gram's Iodine (mordant that fixes crystal violet to cell wall)
  • Decolorizer (e.g. ethanol)
  • Safranin (secondary stain)
  • Water (preferably in a squirt bottle)


    • ]]>     2017-03-06 15:04:35 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044474     https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044868 2017-03-06 15:05:27 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044868 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044935 2017-03-06 15:05:35 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044935 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044943 Gram staining is used to differentiate between groups of bacteria based on their cell walls]]> 2017-03-06 15:05:36 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044943 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044999 Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet.]]> 2017-03-06 15:05:44 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158044999 Violet stained gram-positive cocci and pink stained gram-negative rod-shaped bacteria. Gram-positive bacteria are bacteria that give a positive result in the Gram stain test. Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-colored when seen through a microscope. https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045065 2017-03-06 15:05:54 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045065 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045079 2017-03-06 15:05:55 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045079 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045118 2017-03-06 15:06:00 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045118 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045142 2017-03-06 15:06:04 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045142 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045287
    ]]>     2017-03-06 15:06:26 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045287     a staining technique for the preliminary identification of bacteria, in which a violet dye is applied, followed by a decolorizing agent and then a red dye. The cell walls of certain bacteria, to retain the first dye and appear violet, while those that lose it appear red https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045356 2017-03-06 15:06:36 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045356 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045445 2017-03-06 15:06:46 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045445 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045518 2017-03-06 15:06:58 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045518 how to stain https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045533
  • Crystal violet (primary stain)
  • Iodine solution/Gram's Iodine (mordant that fixes crystal violet to cell wall)
  • Decolorizer (e.g. ethanol)
  • Safranin (secondary stain)
  • Water (preferably in a squirt bottle)

    • ]]>     2017-03-06 15:07:01 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045533     Gram staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safanin. https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045608 2017-03-06 15:07:12 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045608 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045781 The Gram stain is the most important staining procedure in microbiology

    ]]>     2017-03-06 15:07:36 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045781     https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045834
    ]]>     2017-03-06 15:07:43 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045834     https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045835 The process is named after the Danish bacteriologist who originally devised it in 1882]]> 2017-03-06 15:07:43 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045835 History: https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045938 The Gram staining method, named after the Danish bacteriologist who originally devised it in 1882 (published 1884), Hans Christian Gram.
    http://www.uphs.upenn.edu/bugdrug/antibiotic_manual/Gram1.htm]]>     2017-03-06 15:07:58 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045938     https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045954 2017-03-06 15:08:01 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158045954 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046116 2017-03-06 15:08:25 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046116 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046278
    ]]>     2017-03-06 15:08:51 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046278     https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046298 2017-03-06 15:08:54 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046298 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046339 2017-03-06 15:09:01 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046339 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046350 2017-03-06 15:09:02 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046350 In microbiology, gram staining is an important test used because it can determine the presence of bacteria in a sample, as well as differentiate between the two distinct bacteria species, which are gram-positive and gram-negative bacteria. https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046379 2017-03-06 15:09:06 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046379 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046386 2017-03-06 15:09:07 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046386 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046452 2017-03-06 15:09:19 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046452 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046468 2017-03-06 15:09:21 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046468 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046704 2017-03-06 15:09:50 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046704 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158046732


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    La tinción de gram]]>     2017-03-06 15:11:17 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158047372     https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158047434 2017-03-06 15:11:23 UTC https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158047434 https://padlet.com/cnicosia/s7psbh0e0s9s/wish/158047513
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