https://padlet.com/aidilwafi99/rw31522ghcc7 Ebola virus (EBOV), also known as Zaire ebolavirus is one of six known species within the genus Ebolavirus. It can cause a severe and often fatal hemorrhagic fever in humans and other mammals, known as Ebola virus disease (EVD). The Ebola virus vaccine was developed by genetically modified an attenuated vesicular stomatitis virus (VSV) or its scientific name, Indiana vesiculovirus. en-us 2019-09-20 09:57:48 UTC 2025-11-18 07:03:42 UTC hello@padlet.com aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387129419 The viral RNA could be obtained from a bat and be undergoing gel electrophoresis to separate the viral RNA with other macromolecules e.g. DNA or proteins of the infected animals.

]]> 2019-09-20 12:20:01 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387129419 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387131483 Use Reverse transcriptase enzyme, to reverse transcript the viral RNA to complementary DNA (cDNA).]]> 2019-09-20 12:23:56 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387131483 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387132424 Determine the targeted gene that encode the information of viral capsid of the EBOV and cut the gene using restriction enzyme, EcoR1.]]> 2019-09-20 12:25:45 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387132424 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387142963 Cut the recognition sequence at the multiple cloning site (MCS) plasmid with a ribavirin resistance gene by using the same restriction enzyme, EcoR1 to ensure the complementary 'sticky end' between the MCS plasmid and the targeted gene.]]> 2019-09-20 12:42:36 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387142963 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387151178 Introduce the recombinant DNA plasmid into the mammalian eukaryotic cell by undergoing electroporation.]]> 2019-09-20 12:53:57 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387151178 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387152592 The gene of interest of EBOV is linked to the cut MCS plasmid by using DNA ligase. ]]> 2019-09-20 12:55:55 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387152592 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387200111 Infect the transformed mammalian eukaryotic cell with VSV by transduction and let the VSV packages either recombinant DNA or its own DNA.]]> 2019-09-20 14:03:53 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387200111 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387205075 Culture the VSV with human white blood cell (WBC)and let the transducted VSV to infect WBC. The WBC with EBOV antigen could be obtained and could be cultured as a vaccine.]]> 2019-09-20 14:11:04 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387205075 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387206985 Inject the culture to human blood stream and let the antibodies against the WBC with EBOV antigen.]]> 2019-09-20 14:13:22 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387206985 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387482183 The culture is treated with antiviral drug, ribavirin to kill normal VSV present in it since it has no ribavirin resistance gene in its DNA.]]> 2019-09-21 01:31:28 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387482183 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387535812 2019-09-21 10:17:48 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387535812 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387536406 Patient's body has achieved immunity towards EBOV.]]> 2019-09-21 10:21:36 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387536406 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387536695 2019-09-21 10:23:48 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387536695 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387537480 2019-09-21 10:29:37 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387537480 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387538009 2019-09-21 10:33:19 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387538009 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387539145 2019-09-21 10:41:27 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387539145 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387540306 Zaire ebolavirus is a rare but deadly virus that causes fever, body aches, and diarrhea, and sometimes bleeding inside and outside the body.

link: https://www.webmd.com/a-to-z-guides/ebola-fever-virus-infection]]> 2019-09-21 10:50:01 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387540306 aidilwafi99 https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387540917 Indiana vesiculovirus most prominently causes a vesicular disease in domestic animals that resembles foot-and-mouth disease.

link:https://www.sciencedirect.com/topics/neuroscience/vesicular-stomatitis-virus]]> 2019-09-21 10:54:14 UTC https://padlet.com/aidilwafi99/rw31522ghcc7/wish/387540917