Cardiac Testing

Patient completed a treadmill stress test. The patient experienced drastic increases in blood pressure as well as a few premature ventricular contractions (PVCs) on EKG during the exam. One run of ventricular tachycardia was noted. Additional monitoring using a Holter monitor is suggested given these abnormalities and the noted mitral regurgitation (see below). The patient was only able to walk for 8 minutes before the test was concluded – max speed 3.5 mph at an elevation of 2%, and max blood pressure of 190/110.

Test Results:
The results of Urinalysis test indicate: Positive for bacterial infection. Further test need to preformed to identify the type of bacteria.
The results of the Gross Examination of Colony Morphology test indicate: Size of colonies-small, margins-wary, elevation-raised, color-red. Serratia Marcesens.
The results of Gram Staining test indicate: The gram staining process indicates that Anna's sample was gram negative.
The results of the Biochemical test indicate: Indote test- negative, unease-negative, mobility-positive, H2S-negative.
Type of Bacteria Present: Serratia Marcescens.

Diagnosis: Anna Garcia's death was possibly an affect of multiple things. Anna had diabetes and sickle cell anemia and a bacterial infection, Serratia Marcescens. Anna could have possibly been getting up for aspirin to stop pain in her body caused by diabetes when her legs gave out when standing up. Anna could have hit her head on the table and when she hit the floor it could have started up her respiratory track infection. Anna wouldn't have been able to pick herself off the floor due to her legs and eventually died.
Treatment: 

Gross Examination of Colony Morphology- one of the first steps in characterizing and identifying a bacterial culture. Colony morphology is a way scientists can identify bacteria. An individual colony of bacteria is spread directly onto nutrient agar and grows in a Petri dish. Colonies differ in their shape, size, color and texture.
Four basic categories of colony morphology:

Gram Staining-a process by which the cell wall structure of bacteria is determined using a series of reagents. This is considered a differential stain as the differences in bacteria are discovered. Bacteria are either Gram positive (they stain purple) or Gram negative (they stain pink/red). Gram negative bacteria are generally considered to be more dangerous as they have an additional membrane outside of their cell wall that is an endotoxin.

Biochemical Test- For this test we followed the Gram Negative Identification Flowcharts and use the biochemical test results documented in the data table to determine the bacterial species infecting Anna Garcia.

Data Table:

Ornithine Decarboxylase Test- Positive
Citrate Test-Positive
VP test-Positive
Oxidase Test-Negative
Lysine Decarboxylase Test-Positive
H₂S Test-Negative
Pigmentation-Red
Glucose Fermentation Test-Positive
Lactose Fermentation-Negative
Indole Test-Negative
Urease Test-Negative
Nitrate Reduction-Positive
Arabinose Test-Negative
Motility Test-Positive
Gelatin Hydrolysis-Positive
Catalase Test-Positive