https://padlet.com/violet_reena/rj687z2tgfub 14 Feb 2017 FYP. Please take a selfie and right down 3 important points from your reading. 2. Please list down what w agreed from yesterday's discussion. e.g. fun & bee - close carriei, vapor vs LN2, time? en-us 2017-02-14 06:57:29 UTC 2024-04-13 10:46:15 UTC hello@padlet.com violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/153631700 2017-02-14 07:00:34 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153631700 amaliamohddaud https://padlet.com/violet_reena/rj687z2tgfub/wish/153632207 1.vitrification of embryois influenced by concentration and combination of CPAs.
2.time of exposure during vitrification treatment is important to achieved osmotic equilibrium.
3.cell injury will occur during prolonged storage but no significant damage as embryo will restored after 24H of culture.]]> 2017-02-14 07:06:50 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153632207 teohxiupei https://padlet.com/violet_reena/rj687z2tgfub/wish/153632368 1. Principal step for the success of nuclear transfer for cloning is the efficient method of oocyte activation 

2. There are various parthenogenetic activation 

3. Different species will have different effect on different type of chemicals/stimuli/methods chosen ]]> 2017-02-14 07:08:28 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153632368 feifun0728 https://padlet.com/violet_reena/rj687z2tgfub/wish/153632463 1. Close carrier is better than open carrier during vitrification and storage of oocyte amd embryo as the risk of contamination is higher

2. Survival rate can be higher if the warming rate of carriers is higher.

3. Sterilization of LN2 using ultraviolet irradiation is too complicated and not preferrable (in my personal opinion)


]]> 2017-02-14 07:09:13 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153632463 floriavilla94_5 https://padlet.com/violet_reena/rj687z2tgfub/wish/153632467 1. vitrification is a convenient cryopreservation method, either open carrier and closed carrier have similar effect.
2. vapor nitrogen is a safety and newly developed transport method compare to liquid nitrogen.
3. concentration and the volume of cryoprotectant used with effect the survival rate of oocytes and embryo.

]]> 2017-02-14 07:09:16 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153632467 jyjasha https://padlet.com/violet_reena/rj687z2tgfub/wish/153632519 - crypreservation of sperm may lead to decrease in freezing viability and fertilization ability in animals
- the viability and motility of sperm gradually decrease over storage time
- abnormality gradually increases over time
- concentration does not significantly different during the period of storage

]]> 2017-02-14 07:09:39 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153632519 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/153633992 2. mendeley desktop
3. share document

]]> 2017-02-14 07:25:56 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153633992 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/153635565

]]> 2017-02-14 07:38:40 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153635565 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/153637032 2017-02-14 07:51:02 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153637032 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/153640359 2017-02-14 08:20:38 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153640359 feifun0728 https://padlet.com/violet_reena/rj687z2tgfub/wish/153640390 1. We decided to use open carrier as control for vitrification of cleavage stage embryo, but we are wonder open pulled straw is better or cryoloop?

2. Can we have different storage duration to mimic transport of embryo and then see the effect on developmental of embryo for each duration. ( eg. 48hrs/72hrs/96hrs)

3. We observe the developmental of blastocyst under fluorescence microscope after 48 hrs of thawing? Or longer than that?

4. We are using mice or goat?]]> 2017-02-14 08:20:52 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/153640390 teohxiupei https://padlet.com/violet_reena/rj687z2tgfub/wish/154208756 In agreement to use IVF and time as control, using chemicals as stimuli (eg: ethanol, 6-DMAP, A23187) (but chemicals TBC, as I need to further read on it) as varying factor. 

If possible, will try to do freezing & staining 

Question: is it confirmed that I'll be using superovulated mice as my sample?]]> 2017-02-16 02:28:38 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154208756 amaliamohddaud https://padlet.com/violet_reena/rj687z2tgfub/wish/154209254 1. Stable stage of embryo for vitrification : morula
2. will do staining for blastocyst after thawing
3. open carrier for vitrification
4.will assess:
- survivality of embryo after thawing and during culture
-development of morula to blastocyst
5. minimizing as much parameters to be present
6. agree to use no vitrification and no cryopreserved morula for control.
7.time of cryopreserved is the parameter:
-immediately 0 H
-24 H
-168 H (7 days)
8. culture within 48 H and wil be assess for every 4 H
 *can be alter depends on availability 


]]> 2017-02-16 02:32:44 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154209254 feifun0728 https://padlet.com/violet_reena/rj687z2tgfub/wish/154209774 1. We agree to use open carrier instead of close carrier

2. Duration of storage to mimic transport : 48hrs/72hrs/96hrs

3. Not doing oocyte vitrification, in vivo embryo at cleavage stage will be collected and use as our sample.]]> 2017-02-16 02:37:39 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154209774 floriavilla94_5 https://padlet.com/violet_reena/rj687z2tgfub/wish/154210206 2. Duration of  our mimic transport are 48/72/96 hours.
3. We are using embryo at cleavage stage.

]]> 2017-02-16 02:42:28 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154210206 amaliamohddaud https://padlet.com/violet_reena/rj687z2tgfub/wish/154228003 1. we are using mice or ruminant embryo for prolonged storage?
2. does assessment for embryo development within 48 H in every 4 H is somehow quite impossible ? to think that  i have to stay in lab  almost a week for this purpose :D



]]> 2017-02-16 06:31:32 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154228003 jyjasha https://padlet.com/violet_reena/rj687z2tgfub/wish/154771942  1. Use mature bulls
2. Crypopreserved sperm in liquid nitrogen do maintain population especially those of 
endangered species.
3. Storage time does not show significant effect to the characteristics of spermatozoa.
4. Ejaculates must be evaluated for pH, volume, colour, viability, mobility, abnormality and sperm comcentration before amd after cryopeserved.
5. Inter-male variablity give impact to the susceptibility of bull sperm to cryoinduced damage.
6. Storage time divide into two: short term storage and long term storage
Short term : 0h, 24h,48h
Long term : 1 week, 1 month
7. Agree to use two different type of extenders
8. Should have one control sample,





]]> 2017-02-19 03:43:08 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154771942 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/154873456
mendeley reference and there are lotsss more in youtube

]]> 2017-02-20 07:45:49 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154873456 ahkoon_1225 https://padlet.com/violet_reena/rj687z2tgfub/wish/154938125 1. There are two types of cryopreservation procedures: slow freezing and vitrification.
2. Usually storage time does not show significant effect to the fertility of the oocytes, but some studies show longer time may cause changing in quality of the oocytes hence affect the fertility.
3. Contamination may occur in liquid nitrogen due to it did not sterile. ]]> 2017-02-20 14:15:22 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/154938125 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/155074930 Triton X 100
Hoechst
Propidium iodide

]]> 2017-02-21 07:49:25 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/155074930 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/155487425 2017-02-22 15:48:09 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/155487425 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156213914 Hi all, lovely day. Can you find out if we can stain blastocyst with FdA. And which stage. Write in the comment. Thanks!

]]> 2017-02-25 23:53:41 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156213914 sitifariza0526 https://padlet.com/violet_reena/rj687z2tgfub/wish/156224315 1. main advantage to using frozen semen in any breeding program is faster genetic gain for the inherited trait desired. 
2. Fertility rates using frozen stallion spermatozoa are not equal to that of fresh semen
3. As equine semen is a one of the most difficult in the industry to cryopreserve efficiently without causing damage to the membrane or apoptosis. 

]]> 2017-02-26 06:41:56 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156224315 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156408572 2017-02-27 14:20:32 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156408572 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156409047 2017-02-27 14:21:37 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156409047 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156409502 2017-02-27 14:22:30 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156409502 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156410042 2017-02-27 14:23:37 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156410042 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156411858 2017-02-27 14:27:19 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156411858 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156461103 2017-02-27 16:14:21 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156461103 violet_reena https://padlet.com/violet_reena/rj687z2tgfub/wish/156462939 I looove reading old books because they explain theoretical and methodology very detailed. Enjoy reading]]> 2017-02-27 16:18:37 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156462939 amaliamohddaud https://padlet.com/violet_reena/rj687z2tgfub/wish/156624538 I dont know the measurement😂]]> 2017-02-28 05:19:45 UTC https://padlet.com/violet_reena/rj687z2tgfub/wish/156624538