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      <title>Blended Learning 6 by Chuah</title>
      <link>https://padlet.com/acsonchuah/raq4y713kbn</link>
      <description>ABO Discrepancies</description>
      <language>en-us</language>
      <pubDate>2018-01-29 06:11:52 UTC</pubDate>
      <lastBuildDate>2026-03-07 18:07:43 UTC</lastBuildDate>
      <webMaster>hello@padlet.com</webMaster>
      <image>
         <url>https://padlet-assets.s3.amazonaws.com/icons/Rocket.png</url>
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      <item>
         <title>Blended Learning </title>
         <author>acsonchuah</author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225494073</link>
         <description><![CDATA[<div>Write your answer in this way:<br><strong><br>Case:<br>Problem:</strong></div><div><strong>Causes:</strong></div><div><strong>Resolution:</strong></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:14:51 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225494073</guid>
      </item>
      <item>
         <title>Farhan</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495636</link>
         <description><![CDATA[<div><strong>Case 1.</strong><br><em>Case</em>- Group 1 Discrepancies<br><em>Problem</em>- Weak reactivity with B cells<br><em>Causes</em>- Weakly reactivity of antibody<br><em>Resolution</em>- Incubate in 37°c, If negative incubate at 4°c<br><br><strong>Case 2<br></strong><em>Case</em>- Group 2 Discrepancies<br><em>Problem</em>- Weakly reactivity with Anti B<br><em>Causes</em>- Due to missing antigen, or acquired B phenomenon<br><em>Resolution</em>- <br>●Acetic Anhydrate to decrease the reactivity of acquired B<br>●Incubate in 37°c for 30 minutes. If negative, incubate in 4<br><br><strong>Case 3</strong><br><em>Case</em>- Group 3 Discrepancies<br><em>Problem</em>- Pseudoagglutination<br><em>Causes</em>- Rouleax formation (coin stacking red cells)<br><em>Resolution</em>- Wash with saline 3 times to disperse the red cells<br><br><strong>Case 4<br></strong><em>Case</em>- No discrepancies<br><em>Problem</em>- No problem<br><em>Causes</em>- No causes<br><em>Resolution</em>- Patient is normal<br><br><strong>Case 5<br></strong><em>Case</em>- Group 2 Discrepancies<br><em>Problem</em>- No agglutination on Anti A<br><em>Causes</em>- Absence of A antigen<br><em>Resolution</em>- Need to do screening once again<br><br><strong>Case 6<br></strong><em>Case</em>- Group 2 Discrepancies<br><em>Problem</em>- Mixed field<br><em>Causes</em>- Subgroup of B<br><em>Resolution</em>- Incubate with B1 cells at 37°c<br><br><strong>Case 7<br></strong><em>Case</em>- No case<br><em>Problem</em>- Normal patient<br><em>Causes</em>- <br><em>Resolution</em>- <br><br><strong>Case 8<br></strong><em>Case</em>- Group 4 Discrepancies<br><em>Problem</em>- Weak agglutination of B cells<br><em>Causes</em>- Cold antibody or alloantibody<br><em>Resolution</em>- Go for antibody screening<br><br><strong>Case 9<br></strong><em>Case</em>- Group 1 Discrepancy<br><em>Problem</em>- No reaction at reverse grouping<br><em>Causes</em>- Undergoing bone marrow transplant, chemotherapy (antibody supression)<br><em>Resolution</em>- Incubate at room temperature for 15-30 minutes</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:27:42 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495636</guid>
      </item>
      <item>
         <title>Ika :)</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495638</link>
         <description><![CDATA[<div>CASE 1<br>Problem:&nbsp; weaken reaction / missing&nbsp; antibody in reverse grouping&nbsp;<br>Causes: Group 1 Discrepancies - Lack of antibody (AGE RELATED OR WEAKENED IMMUNE SYSTEM)&nbsp;<br>Resolution: Enhance the weak or missing reaction in the serum by incubating the patient serum with reagent A1 and B cells at room temperature for approximately 15 to 30 mins.&nbsp;<br><br>CASE 2&nbsp;<br>Problem:&nbsp;<br>1+ reaction with anti-B. Appears to have additional antigens.<br>Causes:&nbsp;<br>Group 2 Discrepancies - Acquired B antigen<br>Resolution:&nbsp;<br>Patient history - bowel obstruction, carcinoma of colon/rectum (E.coli)<br><br>CASE 3<br>Problem:&nbsp;<br>Weak forward with anti-A and 1+ reaction with A1 cells<br>Causes:&nbsp;<br>1) Subgroup of A (A2 with anti-A1)<br>2) unexpected cold reacting antibody to antigen on reagent A1 cells<br>Resolution:<br>1) test patient cells with anti-A1 lectin and with patient serum test with A2 cells<br>2) an unexpected cold antibody would be detected in the antibody screening&nbsp;<br><br>CASE 4<br>Report normal result.&nbsp;<br><br>CASE 5<br>Problem: Weak reaction in forward grouping / Missing antigen in forward grouping.&nbsp;<br>Cause: A subgroup<br>Resolution: Screening again<br><br>CASE 6<br>Problem: forward grouping&nbsp;<br>Causes: Group B individual transfused with group O cells&nbsp;<br>Resolution: Check for recent transfusion<br><br>CASE 7&nbsp;<br>Problem: normal but weak&nbsp;reaction<br>Causes:&nbsp;blood sample/technical<br>Resolution: Redo the test and check <br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:27:44 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495638</guid>
      </item>
      <item>
         <title>Jocelyn </title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495671</link>
         <description><![CDATA[<div><strong>Case</strong> <strong>1</strong> <br><strong>Problem</strong>: Due to weaken reaction or missing antibody of the reverse grouping (B cells)<br><strong>Causes</strong>: Caused by Group 1 discrepancies which is lack of antibody (Age related or weakened immune system)<br><strong>Resolution</strong>: To enhance the weak or missing reaction in the serum by incubating the patient serum with reagent A1 and B cells at room temperature for approximately 15-30minutes. <br><strong>Case 2</strong><br><strong>Problem</strong>: Due to unexpected reaction or missing antibody in reverse grouping (Anti B)<br><strong>Causes</strong>: Caused by Group 2 discrepancies which is lack of antigens. <br><strong>Resolution</strong>: Patient history: bowel obstruction, carcinoma of colon or rectum <br><strong>Case</strong> 3<strong><br>Problem: </strong>Due<strong> </strong>to weaken forward with anti-A and 1+ reaction with A1cells<br><strong>Causes</strong>: Subgroup of A which is A2 with anti-A1 &amp; Due to unexpected cold reacting antibody to antigen on reagent A1 cells<br><strong>Resolution</strong>: a) Test patient cells with anti-A1 lectin and patient's serum test with A2 cells<br>b)Unexpected cold antibody would be detected in the antibody screening <br><strong>Case</strong> <strong>4<br>Problem: </strong>Normal<strong><br>Causes: -<br>Resolution:-<br>Case 5<br>Problem: </strong>Due to weak reaction in forward grouping. Absence of antigen in forward grouping <strong><br>Causes: </strong>A<strong> </strong>subgroup <strong><br>Resolution: </strong>To enhance the reaction by extending the incubation time <br><strong>Case 6<br>Problem:</strong>Strength of anti-B is weaker than expected in reverse grouping<strong><br>Causes:</strong>Group B individuals transfused with group O cells<strong><br>Resolution: </strong>Is it any recent transfusion <br><strong>Case 7<br>Problem: </strong>Due to weak reaction but normal<strong><br>Causes: </strong>technical problem or blood samples <strong><br>Resolution:&nbsp;</strong>Need to redo and check again&nbsp;<br><strong>Case 8<br>Problem:<br>Causes:<br>Resolution:</strong></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:28:05 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495671</guid>
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      <item>
         <title>Carolyn</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495739</link>
         <description><![CDATA[<div><strong>Case: 1</strong><br><strong>Problem: </strong>Due to the weak reaction at the reverse grouping. <br><strong>Causes</strong>: Group 1 Discrepancies. It is may be due to&nbsp; lack of antibodies.&nbsp;</div><div><strong>Resolution</strong>: Incubated the patient serum with the reagent A1 and B cells at room temperature for 15 to 30 min. If no reaction, serum-cell mixtures can be incubated at 4 degree celcies for 15-30 min.<br><br><strong>Case: 2</strong><br><strong>Problem</strong>: Due to the weak reaction at the forward grouping and unexpected reactions in the forward grouping.<br><strong>Causes:</strong> Group 2 Discrepancies. it may be due to the extra of the antigens. Besides, it may also be having subgroup&nbsp; B or may be acquired phenomenon<br><strong>Resolution:</strong> Incubated the patient serum with the reagent A1 and B cells at room temperature for 15 to 30 min. If no reaction, serum-cell mixtures can be incubated at 4 degree celcies for 15-30 min. <br><br><strong>Case: 3</strong></div><div><strong>Problem: </strong>Due to pseudo agglutination which is the problem occur in the forward and reverse grouping.</div><div><strong>Causes: </strong>Group 2 Discrepancies. It may be causes by subgroup A and unexpected cold antibodies to antigen on reagent A1 cells&nbsp; formation<strong><br>Resolution: </strong>Test the patient cells with A1 lectin and patient serum test with A2 cells and unexpected cold antibody would be detect ed in the antibody screening<strong><br></strong><br><strong>Case: 4</strong><br><strong>Problem:</strong> Normal<br><strong>Causes</strong>: No causes<br><strong>Resolution:</strong> No resolution <br><br><strong>Case: 5</strong><br><strong>Problem:</strong> The anti-A does not clot. It may be weak reaction in forward grouping. There is lack of the antigen<br><strong>Causes:</strong> Group 2 Discrepancies. It may be due to subgroup A. <br><strong>Resolution:</strong>&nbsp; Test with the polyclonal or monoclonal belnd of anti-A and the anti-B<br><br><strong>Case: 6</strong><br><strong>Problem:</strong> unexpected reaction in the forward grouping because the anti-B show clot in the reactions<br><strong>Causes:</strong> Group 2 Discrepancies. It may be cause by the leukemia<br><strong>Resolution</strong>: check for recent transfusion or recent bone marrow transplantation.<br><br><strong>Case</strong>: <strong>7</strong><br><strong>Problem</strong>: Normal<br><strong>Causes</strong>: No causes<br><strong>Resolution</strong>:&nbsp; No resolution<br><br><strong>Case: 8</strong> <br><strong>Problem</strong>: Forward shows AB individual. reverse show weaker extra reaction with B cells<br><strong>Causes</strong>: Group 4 Discrepancies. It may cause by autoantibody or alloantibody<br><strong>Resolution:</strong> Screen for antibodies using Screening Cells and an autocontrol<br><br><strong>Case: 9</strong><br><strong>Problem: </strong>Reverse grouping, missing patient antibody<br><strong>Causes</strong>: Age related or weakened immune system<br><strong>Resolution</strong>: Incubate at room temperature 15-30 minutes and respin. Check patient history.<br><br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:28:46 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495739</guid>
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      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495761</link>
         <description><![CDATA[<div>Case:1<br>Problem: Reverse grouping B-cells result is abnormal as it shows weak reaction. Should be a strong agglutination but results shows 1+ only.<br>Causes: Group 1. Lack or weak reaction of antibody.<br>Resolution:Incubate patient's serum with reagent B at room temperature.</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:28:58 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495761</guid>
      </item>
      <item>
         <title>Lilly</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495762</link>
         <description><![CDATA[<div>Case 1<br>Problem: weaken reaction in reverse grouping&nbsp;<br>Causes: Has depressed antibody production (group 1)<br>Resolution: Enhance the weak or missing reaction in the serum by incubating the patient serum with reagent A1 and B cells at room temperature for 15-30minutes.&nbsp;</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:28:59 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495762</guid>
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      <item>
         <title>yash</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495763</link>
         <description><![CDATA[<div><strong>Case: 1<br>Problem:&nbsp; weaken reaction in reverse grouping</strong></div><div><strong>Causes: has depressed antibody production&nbsp;</strong></div><div><strong>Resolution: incubate patient serum with reagent&nbsp;B cells at room temperature for 15-30 min.</strong></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:28:59 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495763</guid>
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      <item>
         <title>Sarah</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495768</link>
         <description><![CDATA[<div>Case 1<br>Problem: Weakened reaction with B cells in reverse grouping&nbsp;<br>Causes: Group 1 Discrepancies. It may be due to weakly reacting or missing antibodies.<br>Resolution: Incubate B cells-serum at room temperature for 15-30 minutes.&nbsp;<br><br>Case 2<br>Problem: Unexpected reaction in forward grouping as anti-B shows weak reaction<br>Causes: Group 2 Discrepancies. It may be due to the acquired B phenomenon that causes weak reaction with anti-B in forward grouping.<br>Resolution: Add acetic anhydrate to reduce the reactivity of acquired B<br><br>Case 3<br>Problem: Unexpected reaction with A1 cells in reverse grouping as it shows weak reaction.<br>Causes: Group 2 Discrepancies. It may be due to subgroup of A, A2 with anti-A1 that causes agglutination<br>Resolution: Test patient cells with anti-A1 lectin and patient serum with A2 cells<br><br>Case 4<br>Report result as normal.&nbsp;<br><br>Case 5<br>Problem: No reaction in forward grouping.<br>Causes: Group 2 Discrepancies. It may be due to A subgroup<br>Resolution: Extend incubation time to enhance reaction. Test with polyclonal or monoclonal blend of anti-A, and anti-B&nbsp;<br><br>Case 6<br>Problem: Unexpected reaction in forward grouping as anti-B shows weak mixed field reaction<br>Causes: Group 4 Discrepancies. This may be due to transfusion of group O cells<br>Resolution: Check medical history of patient and find out patient's ABO type prior to transfusion<br><br>Case 7<br>Problem: Normal but weak reaction<br>Causes: Technical problem or blood sample problem<br>Resolution: Redo test and check<br><br>Case 8<br>Problem: Unexpected reaction in reverse grouping as B cells show weak reaction.<br>Causes: Group 4 Discrepancies. It may be due to presence of cold alloantibody or autoantibody&nbsp;<br>Resolution: Screen for antibodies using Screening Cells and an autocontrol<br><br>Case 9<br>Problem: No reaction in reverse grouping of group O individual<br>Causes: Group 1 Discrepancies. Age related or weakened immune system<br>Resolution: Incubate at room temperature 15-30 minutes and respin. Check patient history.&nbsp;<br><br>PANG KANG LIAOOOOOOOOOOOOOOOOO GO HOME</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:29:00 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495768</guid>
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      <item>
         <title>Ee Huey</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495817</link>
         <description><![CDATA[<div>Case: Case 1<br>Problem: Weak react with B cells in reverse grouping<br>Causes: Group 1 discrepancies. It may due to weakly reacting or missing antibodies.<br>Resolution: Enhance the weak or missing reaction in the serum by incubating patient's serum with reagent A1 and B cells at room temperature for 15-30 minutes<br><br>Case: Case 2<br>Problem: Anti-B clot in forward grouping.<br>Causes: Group 2. It may due to Acquired B Phenomenon.<br>Resolution: Add acetic anhydrate to decrease the reactively of acquired B but not effect normal B cells.<br><br>Case: Case 3<br>Problem:Weak forward with anti-A1 and A1 cells clot in reverse grouping.</div><div>Causes: Group 3. It may due to pseudoagglutination or rouleaux formation, or subgroup of A (A2 with anti-A1)</div><div>Resolution: Use saline dilution or saline replacement technique will free the cells&nbsp; in rouleaux formation.<br><br>&nbsp;</div><div>Case: Case 4<br>Problem: Normal</div><div>Causes: No causes because is normal</div><div>Resolution: No resolution because is normal<br><br>&nbsp;</div><div>Case: Case 5<br>Problem: No reaction in forward grouping</div><div>Causes: Group 2. It may due to A subgroup.</div><div>Resolution: Increased the association of antibody and antigen by incubating the test mixture at room temperature up to 30 mins.<br><br>&nbsp;</div><div>Case: Case 6<br>Problem: Have mix field reaction with Anti-B in forward grouping.</div><div>Causes: Group 4. It may due to transfusion of group O cells.&nbsp;</div><div>Resolution: Check medical history of patient and find out patient's ABO type prior to transfusion.<br><br>&nbsp;</div><div>Case: Case 7<br>Problem: It normal but weak react with Anti-A and Anti-B in forward grouping.</div><div>Causes: May due do blood sample or technical problem.</div><div>Resolution: Repeat and check and repeat and check and........<br><br>Case: Case 8<br>Problem: Weak react with B cells in reverse grouping.</div><div>Causes: Group 4. It may due to cold react of autoantibodies in red blood cell or unexpected alloantibodies.</div><div>Resolution: Screen for antibodies using Screening Cells and an autocontrol.</div><div>&nbsp;<br>&nbsp;</div><div>Case: Case 9<br>Problem: Have no any reaction with all cells in reverse grouping.</div><div>Causes: Group 1. It may due to age related or weakened immune system.</div><div>Resolution: Incubate at room temperature 15-30 minutes and respin. Check patient history.<br><br>&nbsp;</div><div>Case: Case 10<br>Problem: Close</div><div>Causes: Can go back now</div><div>Resolution:&nbsp; Yeah!!!!!!</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:29:25 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495817</guid>
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      <item>
         <title>Cassandra</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495834</link>
         <description><![CDATA[<div>Case 1<br>Problem: Unexpected reactions in the reverse grouping<br>Causes: Weakly reacting or missing antibodies<br>Resolutions: Incubate the patient's serum (B-cells) at room temperature<br>Case 2<br>Problem: Unexpected reactions in the forward grouping&nbsp;<br>Causes: Acquired B antigen&nbsp;<br>Resolutions: Increase the association of Ab-Ag by incubating the test mixture at room temperature for up to 30 minutes.&nbsp;<br>Case 3&nbsp;<br>Problem: Weak forward with anti-A&nbsp;<br>Causes: Subgroup of A<br>Resolution: Incubate the serum<br>Case 4&nbsp;<br>Problem: It is normal<br>Causes:&nbsp;<br>Resolution: Report the result<br>Case 5<br>Problem: Missing antigen<br>Causes: Weakened A antigen<br>Resolution:&nbsp; Incubate the patient's serum<br>Case 6<br>Problem: Unexpected reactions in the forward grouping<br>Causes: Group B individual transfused with O cells<br>Resolution: Increase the association of Ab-Ag by incubating the test mixture at room temperature for up to 30 minutes<br>Case 7<br>Problem: It is normal but weak reaction<br>Causes: Technical or blood sample<br>Resolution: Redo and check<br>Case 8<br>Problem: Forward shows AB individual<br>Causes: Possible cold allo- or autoantibody<br>Resolution: Screen for antibodies using auto control<br>Case 9<br>Problem: Reverse grouping, missing patient<br>Causes: Age related or weakened immune system<br>Resolution: Incubate at room temperature 15-30 minutes and respin. Check patient history.<br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:29:34 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495834</guid>
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      <item>
         <title>Aina</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495879</link>
         <description><![CDATA[<div>Case 1 :&nbsp; Group 1 discrepancies&nbsp;<br>Problem : Reverse grouping&nbsp;<br>Causes : Group 1 - weakly reacting / missing antibody<br>Resolution: incubate B cells serum patient at room temperature&nbsp;<br><br>Case 2 : Group 2 discrepancies&nbsp;<br>Problem : unexpected reaction Forward grouping&nbsp;<br>Causes : missing antigen / weakly reactivity of reaction&nbsp;<br>Resolution : incubate serum for 30minutes at room temperature&nbsp;<br><br>Case 3 : Group 1 weak forward with anti-a and a1<br>Problem : subgroup of A&nbsp;<br>Causes : unexpected cold reaction antibody&nbsp;<br>Resolution:&nbsp; wash with saline 3 times<br><br>Case 4 : Normal result and report the result<br>Problem :&nbsp;<br>Causes :&nbsp;<br>Resolution:<br><br>Case 5 : Group 2 discrepancies&nbsp;<br>Problem : unexpected reaction in forward grouping&nbsp;<br>Causes : no antigen presence&nbsp;<br>Resolution: need to do antibody screening once again<br><br>Case 6 : Group 2 discrepancies - forward grouping&nbsp;<br>Problem: mixed fields&nbsp;<br>Causes : group B individual transfused with group O<br>Resolution: bone marrow&nbsp;<br><br>Case 7 : Normal .&nbsp;<br>problem : weak reaction<br>Causes : technical of blood sample<br>Resolution: redo and check<br><br>Case 8 : Group 1 - reverse grouping&nbsp;<br>Problem:&nbsp; weak reactivity of B cells&nbsp;<br>Causes: Weak antibody&nbsp;<br>Resolution: incubate serum for longger time at room temperature&nbsp;<br><br>Case 9 : Group 1 discrepancies&nbsp;<br>Problem : reverse grouping, missing patients antibody probably group O with no antibodies&nbsp;<br>Causes : age related&nbsp;<br>Resolution: incubate at room temperature 15-30 minutes and respin. Check patient history.<br><br><br>&nbsp;<br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:29:57 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495879</guid>
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      <item>
         <title></title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495926</link>
         <description><![CDATA[<div>I scrd&nbsp;<br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:30:28 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495926</guid>
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      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495941</link>
         <description><![CDATA[<div>Case 1<br>Problem : unexpected reaction in the reverse grouping due to weakly reacting or missing antibodies<br>Causes : Group 1 discrepancies<br>Resolution : incubate patient's serum<br><br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:30:36 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225495941</guid>
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      <item>
         <title>Shahrul</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225496000</link>
         <description><![CDATA[<div>Case 1 :<br>Problem: Reverse grouping result in B cells is abnormal, because weaken reactions, it is only 1+&nbsp;<br>Cause: Group 1<br>Resolution: incubate patient serum with reagent B at room temperature.&nbsp;<br><br>Case 2 :<br>Problem:&nbsp; weak reactions with mixed-field appearance&nbsp;<br>Causes: Group 2, Acquired B phenomenon&nbsp;<br>Resolution: increase the Ab-Ag by incubate in room temperature about 30 minutes<br><br>Case 3 :<br>Problem: subgroup , weak at forward grouping&nbsp;<br>Causes: Group 1, subgroup A<br>Resolution:&nbsp; incubate the test mixture at 4 degree Celsius, within 15-30 minutes .&nbsp;<br><br>Case 4 :&nbsp;<br>Problem : No prob, Normal<br>Causes: normal&nbsp;<br>Resolution : -&nbsp;<br><br>Case 5 :&nbsp;<br>Problem: weak in forward grouping&nbsp;<br>Causes: Group 2, A subgroup is&nbsp;<br>Resolution: incubate the test mixture in 4 degree Celsius for 15-30 minutes<br><br>Case 6 :<br>Problem: mixed in Anti-B, so its weaker<br>Causes: Group 4, rbc transfusion<br>Resolution: incubate at room temperature<br><br>Case 7 :<br>Problem: Normal but weak reaction<br>Causes: AB blood Group not produce antibody, maybe technical prob&nbsp;<br>Resolution: redo and check&nbsp;<br><br>Case 8 :&nbsp;<br>Problem: weak in reverse grouping, presence of B cells<br>Causes: Group 4, possible cold allo or autoantibody&nbsp;<br>Resolution: screen for antibodies using autocontrol and screening cells<br><br>Case 9 :<br>Problem: might be reverse grouping<br>Causes: Group 1, suppress antibody and weakened immune system<br>&nbsp;Resolution:&nbsp; incubate at room temperatures , 15~30&nbsp;<br><br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:31:10 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225496000</guid>
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      <item>
         <title>I hate immunohaemato but because of mr.chuah I love it....herrmmmm</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225496813</link>
         <description><![CDATA[]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:37:55 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225496813</guid>
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         <title></title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497018</link>
         <description><![CDATA[]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:40:11 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497018</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497276</link>
         <description><![CDATA[<div>Case:2<br>Problem: Slight reaction at forward grouping, Anti-B that shows 1+ which suppose not to occur. This shows presence of Antigen B in the RBC. Might be Acquired B phenomenon.<br>Causes: Group 2. Presence of weak or small amount of antigens.<br>Resolution: Increase the solution pH by using Acetic anhydrate that will change the pH that might cause the Acquired B phenomenon not to react </div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:42:05 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497276</guid>
      </item>
      <item>
         <title>yash</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497339</link>
         <description><![CDATA[<div>&nbsp;</div><div><strong>Case: 2<br>Problem: group 2&nbsp; weaken reaction of forward grouping in B antigen</strong></div><div><strong>Causes: due to presence of Acquired B phenomenon&nbsp;</strong></div><div><strong>Resolution:</strong> bacterial infection of E.coli identification is required<br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:42:35 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497339</guid>
      </item>
      <item>
         <title>Lilly </title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497612</link>
         <description><![CDATA[<div>Case 2<br>Problem:weaken reaction in forward grouping for anti-B<br>Causes: Due to aquired B phenomenon<br>Resolution: increase the association of ab-ag by incubating the test mixture at 4°C for 15-30minutes </div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:44:44 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225497612</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225498470</link>
         <description><![CDATA[<div>Case 2<br>Problem : the unexpected reaction in the forward grouping<br>Causes : Group 2 discrepancies due to lack of antigen<br>Resolution : Acetic anhydrate is to decrease the reactivity of acquired B antigen</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:50:21 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225498470</guid>
      </item>
      <item>
         <title>Case 2</title>
         <author>acsonchuah</author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225498667</link>
         <description><![CDATA[]]></description>
         <enclosure url="https://padlet-uploads.storage.googleapis.com/42349356/1112402fc5e51e5c9479362aa3b4137a/2.jpg" />
         <pubDate>2018-01-29 06:52:12 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225498667</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499121</link>
         <description><![CDATA[<div>Case: 3<br>Problem: Weak reaction of Anti-A yet mild agglutination at A1 cells shows the blood group is a subgroup causing weaken reactions. This is than supported with the strong agglutination at B cells.&nbsp;<br>Causes: Group 2. Subgroup A reactions.(A2 cells)<br>Resolution: Test anti-A1 lectin with patient's cells and A2 cells with patient's serum.</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:55:15 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499121</guid>
      </item>
      <item>
         <title>Case 3</title>
         <author>acsonchuah</author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499123</link>
         <description><![CDATA[]]></description>
         <enclosure url="https://padlet-uploads.storage.googleapis.com/42349356/b3c89a0588bc44a554b29527630dfc07/3.jpg" />
         <pubDate>2018-01-29 06:55:17 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499123</guid>
      </item>
      <item>
         <title>yash </title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499229</link>
         <description><![CDATA[<div>&nbsp;</div><div><strong>Case: 3<br>Problem: weak forward reaction with 1+ A1 cells</strong></div><div><strong>Causes: presence of subgroup A may present</strong></div><div><strong>Resolution:</strong> test A1 lectin with patient's cell<br><br>&nbsp;</div><div><strong>Case:4<br>Problem: normal,normal ,normal</strong></div><div><strong>Causes: none</strong></div><div><strong>Resolution:</strong> report the result<br>&nbsp;</div><div><strong>Case: 5<br>Problem: unexpected missing antigen in forward grouping</strong></div><div><strong>Causes: Leukemias may weakened the A or B antigen</strong></div><div><strong>Resolution: extend incubate patient serum at RT for 30 minutes.<br><br></strong>&nbsp;</div><div><strong>Case: 6<br>Problem: unexpected weak agglutination with mixed field in forward grouping</strong></div><div><strong>Causes: acquired B&nbsp;</strong></div><div><strong>Resolution:</strong> Treat with Acetic anhydrate<br><br>&nbsp;</div><div><strong>Case: 7<br>Problem: normal with weak reaction</strong></div><div><strong>Causes: technical problem or blood sample</strong></div><div><strong>Resolution:</strong> The whole test procedure has to re-do<br><br>&nbsp;</div><div><strong>Case: 8&nbsp;<br>Problem: Unexpected weak reaction with 1+ in B cell</strong></div><div><strong>Causes: Due&nbsp; to presence of cold auto-antibody because it cause clothing at room temperature</strong></div><div><strong>Resolution:</strong> Antibody screening&nbsp;<br><br>&nbsp;</div><div><strong>Case: 9&nbsp;<br>Problem: Missing antibody in reverse grouping which probably blood group O</strong></div><div><strong>Causes: Age related ( infant / older )</strong></div><div><strong>Resolution:</strong>&nbsp; incubate in RT for 15-30 minutes.<br><br>&nbsp;</div><div><strong>Case: 10<br>&nbsp;no patient!! counter CLOSED!!!!!!</strong></div><div>&nbsp;</div><div><strong><br></strong>&nbsp;</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:56:09 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499229</guid>
      </item>
      <item>
         <title>Lilly</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499686</link>
         <description><![CDATA[<div>Case 3<br>Problem:weaken reaction with anti-A and A1 cells<br>Causes: subgroup of A( A2 with anti -a1)<br>Resolution: test patient cells with anti -a1 lectin and with patient serum test with A2 cells&nbsp;<br><br>Case 4<br>Problem: no problem. Normal<br>Causes:<br>Resolution<br><br>Case 5<br>Problem: Due to the missing antigens in forward grouping&nbsp;<br>Causes: Leukemias mat yield weakened A or B antigens or the A subgroup&nbsp;<br>Resolution: increasethe association of ab-ag by incubating the patient serum at room temperature for 30minutes.<br><br>Case 6<br>Problem: unexpected weak agglutination of antigen with mix fields&nbsp;<br>Causes: acquired B<br>Resolution: Find what ABO type the patient prior with transfusion or treat with Acetic anhydrate to decrease cell reactivity of aquired B<br><br>Case 7<br>Problem: normal but weak reaction </div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 06:59:13 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225499686</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225500145</link>
         <description><![CDATA[<div>Case 3<br>Problem : weak forward with anti-A and 1+ reaction with A1 cells<br>Causes : Group 3 discepancies<br>Resolution : test patient cell with anti A1 lectin and with patient serum A2 cells</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:02:08 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225500145</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225501409</link>
         <description><![CDATA[<div>Case:4<br>Problem: Nothing, its normal<br>Causes: -<br>Resolution: - </div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:11:00 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225501409</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225501492</link>
         <description><![CDATA[<div>Case 4<br>Problem :normal😏<br>Causes :&nbsp;-😏<br>Resolution :&nbsp;-😏</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:11:46 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225501492</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225502534</link>
         <description><![CDATA[<div>Case: 5<br>Problem: No reaction at Anti-A which determines the absence of antigen A. The B cells reaction shows 3+.<br>Causes: Group 2. No antigen A present, subgroup of A.<br>Resolution: Test patient's cells with Anti-A serum for 15 - 30 minutes under 4 degrees Celsius.  </div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:19:44 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225502534</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225502601</link>
         <description><![CDATA[<div>Case 5<br>Problem : unexpected reaction in&nbsp; the forward grouping<br>Causes : subgroup A may be present<br>Resolution : incubate</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:20:09 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225502601</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225504310</link>
         <description><![CDATA[<div>Case: 6<br>Problem: Presence of MF reaction at Anti-B.<br>Causes: Group 4. Blood transfusion<br>Resolution: Patient's latest blood transfusion record is revised.</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:29:06 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225504310</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225504770</link>
         <description><![CDATA[<div>Case&nbsp;6<br>&nbsp;Problem: unexpected reaction in forward grouping<br>Causes:Leukemia that weakened A or B antigen<br>Resolution: recent transfusion</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:31:31 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225504770</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225505477</link>
         <description><![CDATA[<div>Case: 7<br>Problem: Normal yet weak reaction<br>Causes: -<br>Resolution: Redo the sample and check again.</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:35:04 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225505477</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225505544</link>
         <description><![CDATA[<div>Case 7<br>Problems : normal!!!!<br>Causes : technical problem<br>Resolution : check again</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 07:35:28 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225505544</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225515050</link>
         <description><![CDATA[<div>Case: 8<br>Problems: Weak reaction occured at B cells when it can be said the current patient's blood group is AB by forward grouping.<br>Causes: Group 4. Presence of autoantibody or alloantibody. <br>Resolution: Perform cell screening using screening cells and an autocontrol.</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 08:18:56 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225515050</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225515617</link>
         <description><![CDATA[<div>Case 8<br>Problem: due to miscellaneous problems<br>Causes: Group 4 discrepancies.cold reactive autoantibodies.<br>Resolution: screening for antibodies</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 08:21:29 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225515617</guid>
      </item>
      <item>
         <title>Lilly</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225516685</link>
         <description><![CDATA[<div>Case 8<br>Problem: extra reaction with B cells in forward grouping&nbsp;<br>Causes: possible cold allo or auto antibody from another blood group system<br>Resolution: screen for antibodies using screening cells and autocontrol</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 08:26:01 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225516685</guid>
      </item>
      <item>
         <title>Lilly</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225518562</link>
         <description><![CDATA[<div>Case 9<br>Problem: reverse grouping, missing antibody probably group O with no antibodies&nbsp;<br>Causes: age related or weakened immune system&nbsp;<br>Resolution: incubate at room temperature&nbsp; for 15-30minutes and respin. Check patient history.<br><br><br></div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 08:33:33 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225518562</guid>
      </item>
      <item>
         <title>SRI</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225518842</link>
         <description><![CDATA[<div>Case: 9<br>Problem: This patient has no forward nor reverse grouping reactions. <br>Causes: Group 1. The patient is undergoing certain immunosuppresive medication due to a bone marrow or organ transplantation.<br>Resolution: Incubate at room temperature for 15-30 minutes. Check patient's medical history for reconfirmation.  </div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 08:34:54 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225518842</guid>
      </item>
      <item>
         <title>Nadya</title>
         <author></author>
         <link>https://padlet.com/acsonchuah/raq4y713kbn/wish/225520514</link>
         <description><![CDATA[<div>Case 9<br>Problem:missing antibody<br>Causes:age related<br>Resolution : incubate at room temperature and check patient's history</div>]]></description>
         <enclosure url="" />
         <pubDate>2018-01-29 08:41:30 UTC</pubDate>
         <guid>https://padlet.com/acsonchuah/raq4y713kbn/wish/225520514</guid>
      </item>
   </channel>
</rss>
