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      <title>Genetics of Microorganism by Leonardo Emanuel de Oliveira Costa</title>
      <link>https://padlet.com/leonardo_costa/r8st67oloods</link>
      <description>Let´s make one wonderfull review of genetics of bacteria in english!!!</description>
      <language>en-us</language>
      <pubDate>2018-06-15 11:17:06 UTC</pubDate>
      <lastBuildDate>2023-03-01 18:18:29 UTC</lastBuildDate>
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         <title>Bacterial Chromosome</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267376554</link>
         <description><![CDATA[<div>  </div><div>Every organism has genetic information, which is responsible for determining all of its characteristics. In most of the cases, this information is stored in DNA. The DNA is compacted, forming what we call as chromosome. Bacteria usually have only one chromosome composed of double‐stranded DNA in a loop. In this case, the DNA doesn´t have a membrane that involves it and its replication, differently of other organisms, is semiconservative. <br><br></div><div>            When compared to human chromosomes, the bacterial one doesn´t have introns, which are non-coding sequences of genetic matter. It may be a problem because it avoids mutation complications. Due to the fact of human chromosomes having a big amount of introns, they are less susceptible to U.V. mutations, what decreases the chance of developing cancer. <br><br></div><div>            Besides this difference, we can also mention the divergence between the genetic matter size of human and bacteria. Considering that the mankind has 46 chromosomes and the bacterium only one, the bacterium´s genome is smaller than the human one. We can also justify the size with the fact mentioned before about the non-existence of introns in bacterial chromosome. <br><br></div><div>            Although we said the bacteria have only one looped chromosome, there are two exceptions, the <em>vibrio cholarae</em>, that has 2 looped chromosomes, and the <em>agrobacterium tumefaceis </em>has a linear chromosome. <br><br></div><div><figure class="attachment attachment--preview"><img src="https://lh4.googleusercontent.com/-ewaXTh_NKh4/TW5rUJ_NuHI/AAAAAAAAAEA/Ia-XVJjuzEc/s1600/plasmideo.gif" width="330" height="404"><figcaption class="attachment__caption"></figcaption></figure> <br><br><br></div>]]></description>
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         <pubDate>2018-06-15 14:11:06 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267376554</guid>
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         <title>DNA replication in bacteria</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267376784</link>
         <description><![CDATA[<div>DNA replication consists of a series of processes that results in the doubling the quantity of genetic information in a single bacteria, in order to generate another individual.<br><br>The first step to replicate DNA is to sign where the process should start. For this, the bacterial metabolism utilizes 'initiator proteins', that act as an origin for the whole process. Look for Photo A.<br><br>When the other enzymes recognize those proteins, one of them (called DNA topoisomerase) uncoils the alfa-helix structure of DNA and, at thye same time, reduces the tension cause by the dissociation of the hydrogen bonds that keep the nitrogenous bases united, which the helicase enzyme is responsible for.<br><br>Then, another enzyme called DNA-polimerase III induces the formation of phosphodiester bonds between free-roaming nucleotides in the nucleus. This process, however, is not perfect and leaves some flaws in polimerization behind. These flaws are called Okazaki fragments, and they are repaired by the DNA-ligase.<br><br>This process is important due to the fact that it is only through it that you can generate genetic material to the daughter cells.<br><br>References:<br><a href="https://www.ncbi.nlm.nih.gov/pubmed/23818497">https://www.ncbi.nlm.nih.gov/pubmed/23818497</a><br><br><a href="https://www.infoescola.com/genetica/replicacao/">https://www.infoescola.com/genetica/replicacao/</a></div>]]></description>
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         <pubDate>2018-06-15 14:12:32 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267376784</guid>
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         <title>Horizontal transfer of genes mechanisms</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267376893</link>
         <description><![CDATA[<div>Horinzontal transfer of genes is one way to share material between two multi or unicellurar organisms of different species. It's the most important kind of DNA sharing that allows bacterial antiobiotic resistence spread through them. This specfic kind of tranference was dicovered about 70 years ago.<br>Nowadays there are 4 different kinds of DNA transference: <br>1 - Bacterial Conjugation:<br>This process consists in a direct contact between two cells and then one injects a conjugative or mobilizable genetic element, often a plasmid or transposon. The cell that contains the plasmid is called F<sup>+</sup> and the one that receives it is called F<sup>-</sup> and, after the transference the one that didn't had the gene will received it becoming one F<sup>+</sup>. <br>Moreover there are two different kinds of F factor that can be formed, Hfr and F'', the first one envolves Hfr and F<sup>-</sup> then the F<sup>-</sup> is transformed into Hfr, the second one F' and F<sup>-</sup> where the F<sup>-</sup> is transformed in F''.<br>2 - Transduction: </div><div> Transduction is the pioneer seen in studies between <em>Salmonella sp</em> bacteria. Later was clear that several different kinds of bacterial virus can serve as natural vectors for genes of their own holts. Generally, transduction propagates progeny viral particales contain the host chromossome rather than a reproduced viral genome. </div><div>Transduction has the lytic and lysogenic cicle. The lytic cicle consists in adsorption, penetration, replication, assembly and release. The lysogenic cicle is about adsorption, penetration, integration and replication. </div><div>There are 2 kinds of transduction: </div><ol><li>Generalized: consists in any bacterial fragment of chromossome being transferred.</li><li>Specialized: is the transferance of genes adjacent to the prophet.</li></ol><div>3 - Transformation: </div><div>In thansformation the donator has his DNA in fragmented form spelled for other bacteria in the same enviroment that the first one is, after that the receptor will identify the DNA and place it in it's DNA, recognizing it. The change of animal cells by tumor-inducing virus can also be called transformation. <br><strong>References:</strong><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187160/">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187160/</a><br><a href="https://www.britannica.com/science/horizontal-gene-transfer">https://www.britannica.com/science/horizontal-gene-transfer</a><br><a href="https://www.britannica.com/science/transformation-biology">https://www.britannica.com/science/transformation-biology</a></div>]]></description>
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         <pubDate>2018-06-15 14:13:19 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267376893</guid>
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         <title>Cromossomo Bacteriano</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377028</link>
         <description><![CDATA[<div> </div><div>Cromossomo Bacteriano é constituído  na maioria das vezes por uma única molécula de DNA fita dupla, circular, não apresenta membrana nuclear e seus genes são Haploides (um único conjunto de genes), e é capaz de autoduplicação. Seus genes contém todas as informações necessárias à sobrevivência da célula.<br>  </div><div>Os genes são organizados na forma de Operons. Os operons são agrupamentos de genes estruturais localizados em sequência no cromossomo bacteriano, que são regulados em conjunto <br><br></div><div>      Um exemplo que podemos destacar é o DNA da E. coli, que tem cerca de 4,6 milhões de pares de bases e possui cerca de 1 mm de comprimento, mil vezes maior que toda a célula. O cromossomo ocupa apenas cerca de 10% do volume celular e seu DNA é superenovelado. <br><br>Como citado a cima , as bacterias possuem apenas 1 cromossomo circular,porém existem algumas exceções como no caso da bactéria Vibrio Cholarae,(que possui 2 cromossomos circulares) e a Agrobacterium Tumefacéis (que possui um cromossomo linear).<br><br></div><div>     Diferente do cromossomo bacteriano, o cromossomo humano contém íntrons e com isso se tornam mais difíceis as mutações genéticas   , são partes que não possuem material genético. Os eucariontes possuem múltiplos cromossomos lineares dentro do núcleo celular, além disso o cromossomo humano possui diferentes formas diferentemente do bacteriano que é circular, são diploides (possui 2 conjuntos de cromossomos completos) no caso dos humanos eles possuem 23 pares. <br>Referencias :<br><a href="https://www1.folha.uol.com.br/fsp/ciencia/fe0308200001.htm">https://www1.folha.uol.com.br/fsp/ciencia/fe0308200001.htm</a><br><a href="http://www.ufjf.br/microbiologia/files/2013/05/Gen%C3%A9tica-Bacteriana-1.pdf">http://www.ufjf.br/microbiologia/files/2013/05/Gen%C3%A9tica-Bacteriana-1.pdf</a><br><br></div>]]></description>
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         <pubDate>2018-06-15 14:14:24 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377028</guid>
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      <item>
         <title>Mecanismo de transferência horizontal de genes</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377053</link>
         <description><![CDATA[<div>A transferência horizontal de genes é o processo pelo qual ocorre a transferência do ADN ( material genético bacteriano) de uma bactéria para outra que não seja da mesma espécie. Esse recurso auxilia na adaptação desses microrganismos e tem grande influência na variabilidade genética dos mesmos. <br>Atualmente, há três mecanismos de transferência conhecidos:<br>1- Conjugação: Envolve o contato entre as duas células. Os plasmídeos de natureza F permitem a transferência de DNA plasmidial de uma célula para outra ( capacidade conjugativa). A célula portadora do plasmídeo ( doadora) é denominada F+ e a que não possui o plasmídeo (receptora) é denominada F- . Dessa forma, quando um plasmídeo é transferido de um doador F+ para um receptor F-, a célula F- é convertida em uma célula F+. Além disso,  a inserção do fator F no cromossomo resulta em uma célula Hfr. <br>( Observe o esquema na imagem 1). <br> 2- Transdução: Transferência feita com o auxílio de vírus. Pode ser generalizada ou especializada.<br>2.1- Generalizada: Pode transferir qualquer segmento do cromossomo bacteriano. (Figura 2.1)<br>2.2- Transferência de genes adjacentes profago. ( Figura 2.2) <br>3-  Tranformação: Dá-se quando absorve-se fragmentos de DNA presentes no meio, encorporando- os. Pode ocorrer com cepas de diferentes espécies e é necessário possuir sítios de superfície para a ligação com o DNA. (Figura 3)<br>Referências:<br><a href="http://www.esalq.usp.br/departamentos/lgn/pub/seminar/MCQuecine-200701-Resumo.pdf">http://www.esalq.usp.br/departamentos/lgn/pub/seminar/MCQuecine-200701-Resumo.pdf</a><br><br><a href="http://www.ebah.com.br/content/ABAAABbNIAD/processos-transferencia-genes-entre-bacterias">http://www.ebah.com.br/content/ABAAABbNIAD/processos-transferencia-genes-entre-bacterias</a><br><br><a href="https://djalmasantos.wordpress.com/2014/03/10/transformacao-bacteriana/">https://djalmasantos.wordpress.com/2014/03/10/transformacao-bacteriana/</a></div>]]></description>
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         <pubDate>2018-06-15 14:14:36 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377053</guid>
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         <title>O que é o DNA?</title>
         <author>gabimartins999</author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377059</link>
         <description><![CDATA[<div>O DNA é uma molécula relacionada diretamente  com as características físicas e fisiológicas de seres vivos. Nos humanos, essa molécula mede aproximadamente 1, 99 m e é composta por fita dupla. Inclusive, este DNA é constituído por regiões não codificadoras (íntrons) e regiões codificadoras (éxons) que formam os genes. Esse último está atrelado à transferência de características comuns para os descendentes, ou seja, à hereditariedade. As bactérias, uma das representantes do reino monera, possuem o DNA com as seguintes características: <br>Circular<br>Fita dupla<br>Replicação semiconservativa<br>Não apresenta carioteca<br>Genoma haplóide<br>Não possui ìntrons<br>Essas características foram descobertas ao longo do tempo, por diversos cientistas. Além disso, foi determinado que essa molécula por nucleotídeos, que são constituidos de um  grupo fosfato, uma pentose e uma base nitrogenada (citosina, guanina, timina e adenina). Entre as principais diferenças entre o DNA e RNA, isto é, uma classe de moléculas  responsáveis pela <strong>síntese de proteínas</strong> das células do seres vivos; pode-se citar a diferença entre as pentoses que as compõem, podendo haver,  no caso do RNA, uma hidroxila no carbono 2' (ribose) ou um hidrogênio no carbono 2' (desoxirribose) no caso do DNA. <br>Ademais, o RNA possui a base nitrogenada uracila ao invés da timina, presente no DNA, e é formado por uma fita simples. As purinas, representadas pela adenina e guanina fazem pareamento com as pirimidinas, grupo constituído pela citosina, timina e uracila. É importante destacar que, no caso do DNA, a citosina pareia com a guanina por três ligações de hidrogênio e a adenina com a timina por duas ligações de hidrogênio.<br>O DNA é passível a mutações ao decorrer do tempo devido a agentes mutagênicos como a radiação ultravioleta, ocasionando dímeros de timina; agentes químicos, por exemplo, o ácido nítrico, que altera a conformação das bases e, finalmente, os agentes biológicos como os elementos transponíveis capazes de se auto-replicar e se  movimentar de uma região para outra em um genoma de uma célula , promovendo, assim a alteração da expressão gênica.<br><br>Referências:<br><a href="https://brasilescola.uol.com.br/o-que-e/biologia/o-que-e-dna.htm">https://brasilescola.uol.com.br/o-que-e/biologia/o-que-e-dna.htm</a><br>Livro Princípios de bioquímica de Lehninger<br><br></div>]]></description>
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         <pubDate>2018-06-15 14:14:39 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377059</guid>
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         <title>What is DNA?</title>
         <author>gabimartins999</author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377101</link>
         <description><![CDATA[<div>DNA is a molecule directly related to the physical and physiological characteristics of living organisms. In humans, this molecule measures approximately 1.99 m and is composed by double strand. In addition, this DNA is made up of non-coding regions (introns) and coding regions (exons) that form the genes. This one is related to the transfer of characteristics common to the offspring, it means, heredity. Bacteria, one of the representatives of the monera kingdom, have DNA with the following characteristics:<br>-Circular;<br>-Double strand;<br>-Semiconservative replication;<br>-There is no carioteca;<br>-Haploid genome;<br>-There is no ìntrons;<br>These characteristics were discovered over time by several scientists. In addition, this molecule was determined by nucleotides, which are constituted of a phosphate group, a pentose and a nitrogenous base (cytosine, guanine, thymine and adenine). Among the major differences between DNA and RNA,  a class of molecules responsible for the synthesis of proteins from living cells; we can mention the difference between the pentoses that make them up, and in the case of RNA, a hydroxyl in carbon 2 '(ribose) or a hydrogen in the carbon 2' (deoxyribose) in case of DNA.<br>In addition, RNA has the uracil base instead of thymine, present in the DNA, and is formed by a simple strand. Purines, represented by adenine and guanine, are paired with pyrimidines, a group consisting of cytosine, thymine and uracil. It is important to note that, in the case of DNA, cytosine is connected to guanine by three hydrogen bonds and adenine to thymine by two hydrogen bonds.<br>DNA is susceptible to mutations over time due to mutagenic agents such as ultraviolet radiation, causing thymine dimers; chemical agents, for example nitric acid, which alters the conformation of the bases and finally biological agents as the transposable elements capable of self-replicating and moving from other region in a genome of a cell, thus altering gene expression.<br><br>References:<br><a href="https://brasilescola.uol.com.br/o-que-e/biologia/o-que-e-dna.htm">https://brasilescola.uol.com.br/o-que-e/biologia/o-que-e-dna.htm</a><br>Lehninger book</div>]]></description>
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         <pubDate>2018-06-15 14:14:59 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377101</guid>
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         <title>How works the transcriptions in bacteria?</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377232</link>
         <description><![CDATA[<div><br> INTRODUCTION:<br><br></div><div>The transcription is an importante  <br>phase, responsible for the synthesis of a RNA molecule from a DNA template. This process involves, basically, three steps: initiation, elongation and termination. <br><br>TRANSCRIPTION:<br><br>The initiation consists in the DNA double helix being unziped by an enzyme, called RNA polymerase, that  binds in a DNA sequence (the promoter) </div><div>to initiate transcription. </div><div>Once RNA polymerase is in position at the promoter, the elongation cans begin. Basically, elongation is the stage when the RNA strand gets longer, thanks to the addition of new nucleotides.<br><br></div><div>During elongation, RNA polymerase "walks" along one strand of DNA, known as the template strand, in the 3' to 5' direction. For each nucleotide in the template, RNA polymerase adds a matching (complementary) RNA nucleotide to the 3' end of the RNA strand. It's important to know that during this process each T of the coding strand is replaced with a U in the RNA transcript. <br><br>Finally, the last step is the termination, wich the mRNA that is formed leaves the nucleous.  RNA synthesis continues until the polymerase encounters a termination signal, at which point transcription stops, the RNA is released from the polymerase, and the enzyme dissociates from its DNA template.  The formation of such a self-complementary structure in the RNA disrupts its association with the DNA template and terminates transcription. Because hydrogen bonding between A and U is weaker than that between G and C, the presence of A residues downstream of the inverted repeat sequences is thought to facilitate the dissociation of the RNA from its template. Other types of transcription termination signals, in both prokaryotic and eukaryotic cells,<strong> </strong>depend on the binding of proteins that terminate transcription to specific DNA sequences, rather than on the formation of a stem-loop structure in the RNA. <br><br>Image that resume the process of the transcription:</div>]]></description>
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         <pubDate>2018-06-15 14:16:13 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267377232</guid>
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         <title>Replicação de DNA bacteriano</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267380047</link>
         <description><![CDATA[<div> A replicação do DNA bacteriano inicia-se pela descondensação da molécula, num processo bi-direcional, ocorrendo nas duas fitas de DNA da dupla-hélice. Esse processo é semelhante à replicação do DNA animal, pois é necessária a utilização de enzimas para descondensá-lo, como, por exemplo, as helicases que desfazem as interações entre as bases nitrogenadas, resultando na separação das fitas.  Após a passagem desta última enzima, proteínas estabilizam o DNA desnovelado; uma DNA-polimerase passa por entre as fitas descondensadas, polimerizando os nucleotídeos livres, adicionando as bases nitrogenadas necessárias para realizar as interações, pontes de hidrogênios. Entretanto, há fragmentos de DNA que não são totalmente polimerisados, os denominados fragmentos de Okazaki. Para acertar este problema, a DNA-ligase encuba-se de passar, novamente, por toda a fita de DNA que estava sendo sintetizada a fim de relê-la, adicionando os nucleotídeos que estão faltando nos fragmentos.<br> <br>A importância desse processo é a duplicação de seu DNA, para que ela possa se reproduzir e assim dá continuidade à sua informação genética. </div><div><br>Referencia bibliografica<br><a href="http://www.ufjf.br/microbiologia/files/2013/05/Gen%C3%A9tica-Bacteriana-1.pdf">http://www.ufjf.br/microbiologia/files/2013/05/Gen%C3%A9tica-Bacteriana-1.pdf</a><br><br><a href="https://netnature.wordpress.com/2016/05/18/replicacao-de-dna-em-procariotos-a-origem-da-mitose-nos-pre-cariotos/">https://netnature.wordpress.com/2016/05/18/replicacao-de-dna-em-procariotos-a-origem-da-mitose-nos-pre-cariotos/</a> </div>]]></description>
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         <pubDate>2018-06-15 14:37:20 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267380047</guid>
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         <title>Photo A (DNA replication)</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267381859</link>
         <description><![CDATA[<div><br></div><div>The blue line represents the original DNA strands and the red lines represent how the copied DNA should form. We call this proteins primaseses</div>]]></description>
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         <pubDate>2018-06-15 14:50:29 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267381859</guid>
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         <title>Como ocorre o processo de transcrição?</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267383056</link>
         <description><![CDATA[<div> </div><div>INTRODUÇÃO <br><br></div><div>A transcrição bacteriana é uma importante etapa para a síntese de proteínas nestes procariotos. A <em>Escherichia Coli,</em> por exemplo, possui uma proteína que inibe o ataque de células do Sistema Imunológico Humano. Este processo é constituído basicamente de cinco etapas, e seu produto consiste numa molécula de RNA mensageiro, que será posteriormente traduzida para a formação proteica. <br><br></div><div>TRANSCRIÇÃO <br><br></div><div>Na transcrição, a partir de uma fita molde de DNA, ocorre a síntese de uma fita complementar de RNA, a partir de um gene específico. Desta forma, a informação genética contida na sequência de bases nitrogenadas do DNA é reescrita, apresentando então a mesma sequência de bases no RNA mensageiro (RNAm). <br><br></div><div>O pareamento específico das bases nitrogenadas ocorre da seguinte forma: As adeninas, que se pareiam com timina no DNA molde, quando transcritas para o RNAm se pareiam com a base uracila. As guaninas, por outro lado, mantêm seu pareamento com as citosinas, exatamente como no DNA. <br><br>O esquema abaixo explicita o processo de transcrição e as estruturas envolvidas.<br><br>Referências bibliográficas:<br>TORTORA, G.J.; FUNKE, B.R.; CASE, CL. Microbiologia. 8. ed., Porto Alegre: Artmed, 2005<br>PORTAL EDUCAÇÃO. Transcrição em procariotos e tradução. Disponível em: <a href="https://www.portaleducacao.com.br/conteudo/artigos/biologia/transcricao-em-procariotos-e-traducao/23359">https://www.portaleducacao.com.br/conteudo/artigos/biologia/transcricao-em-procariotos-e-traducao/23359</a> Acesso em 15/06/2018 às 11:15h.</div>]]></description>
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         <pubDate>2018-06-15 15:00:45 UTC</pubDate>
         <guid>https://padlet.com/leonardo_costa/r8st67oloods/wish/267383056</guid>
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         <title>Figura 1</title>
         <author></author>
         <link>https://padlet.com/leonardo_costa/r8st67oloods/wish/267384184</link>
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         <pubDate>2018-06-15 15:10:08 UTC</pubDate>
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         <title>Figura 2.1</title>
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         <title>Figura 3</title>
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         <pubDate>2018-06-15 15:11:28 UTC</pubDate>
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         <author>gabimartins999</author>
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         <pubDate>2018-06-15 15:11:42 UTC</pubDate>
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         <title>Photo B (DNA replication)</title>
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         <pubDate>2018-06-15 15:15:56 UTC</pubDate>
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