https://padlet.com/anisz2508/pv99qo4ceg6w place what you have found about the microbe here. and comment :) en-us 2017-03-09 12:24:40 UTC 2025-12-28 08:04:32 UTC hello@padlet.com ainaardani https://padlet.com/anisz2508/pv99qo4ceg6w/wish/160742704 My name is Nur Aina Ardani binti Mohd Arif, a first year Biochemistry's student from UPM. I learnt a lot about our team's chosen bacteria; Ideonella Sakaiensis that has intriguing ability of eating plastic, which is considered as non-biodegradable material.  It is able to utilize Poly(ethylene terephthalate)  a.k.a PET as its major energy and carbon source. The journal enclosed here further explains about PET assimilation.]]> 2017-03-17 10:22:09 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/160742704 anisz2508 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/160980841 hey guys, this is how the padlet should look like. place each of your findings here. the rest please do comment on what you think about the material. 
this is my journal. this journal tells how they inoculate the Ideonella Sakaiensis. 

]]> 2017-03-19 10:49:35 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/160980841 aqilahvictorious6 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/162587843 Ideonella sakaiensis was identified in 2016 by a team of researchers from Kyoto Institute of Technology and Keio University after collecting samples of PET debris in a search for bacteria which relied on the plastic for carbon growth. The bacterium was observed to utilize two distinct enzymes that reacted with water to break down PET plastics into terephthalic acid and ethylene glycol, substances which on their own pose no threat to the environment.

Further investigation identified an enzyme, ISF6_4831, which works with water to break down PET into an intermediate substance, which is then further broken down by a second enzyme, ISF6_0224. The scientist explained that by screening natural microbial communities exposed to PET in the environment, we isolated a novel bacterium, Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. When grown on PET, this strain produces two enzymes capable of hydrolyzing PET and the reaction intermediate, mono(2-hydroxyethyl) terephthalic acid.Both enzymes are required to enzymatically convert PET efficiently into its two environmentally benign monomers, terephthalic acid and ethylene glycol

Link that explain more about how they identify Idenonella sakaiensis: http://www.bio.keio.ac.jp/labs/kmiyamoto/Yoshida%20et%20al.%20Science%20(2016)%20Accepted%20Version.pdf ]]> 2017-03-25 22:19:08 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/162587843 lehonphang https://padlet.com/anisz2508/pv99qo4ceg6w/wish/162838665 Hey, these are some information that I found to get to know Ideonella sakaiensis. They talk about the researches done by the researchers team from Japan. ]]> 2017-03-27 14:47:40 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/162838665 zhenglim1995 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/163648067 http://acsh.org/news/2016/07/13/some-hope-for-plastics-in-the-environment

]]> 2017-03-30 09:56:34 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/163648067 limyuyan1995 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/163875504 For addition information, I just found out something about why Ideonells Sakaiensis was placed under the Genera Ideonella. Ideonella is recognised to be related genotypically , for example, as determined by 16S rRNA gene sequence analysis to genera Aquabacterium, Lepothrix and Roseateles within the Betaproteobacteria. This determine the characterictics of bacteris Ideonella Sakaiensis as well as  Ideonella dechloratans and Ideonella azotifigens in the same genera. Below attachement is about Ideonella dechloratans and lets explore the beauty of genera Ideonella.]]> 2017-03-31 04:48:41 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/163875504 lksoon94 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164080370 There are only one research so far which is carry out by a Japanese team, led by Dr. Kohei Oda from the Kyoto Institute of Technology and Dr. Kenji Miyamoto from Keio University.
Before this , scientist only found very few species of fungi but no bacteria function in breaking down poly[ethylene terephthalate], or PET.

Although ideonella sakaiensis have ability to break down PET , the process takes a long time about 6 weeks at 30°C to fully degrade a thumb-nail-sized piece of PET. so, in order to break down world’s plastic pollutants, ideonella sakaiensis have to be improve to become more powerful.
 

These three link is summary of the research on Ideonella sakaiensis which easily to let you understand the concept of the research.
1) http://www.sci-news.com/biology/ideonella-sakaiensis-bacterium-can-break-down-metabolize-plastic-03693.html

2 ) https://www.aaas.org/news/science-newly-identified-bacteria-break-down-tough-plastic

3) https://www.newscientist.com/article/2080279-bacteria-found-to-eat-pet-plastics-could-help-do-the-recycling/]]> 2017-04-01 01:51:30 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164080370 MinnAnan https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164138931 My part covers on the taxonomy of ideonella sakaiensis.
A bacteria that basically digest plastics.
Idenoella sakaiensis is Gram-negative, aerobic, and rod-shaped. It does not form spores. The individual cells of the organism are motile and have a single flagellum. I. sakaiensis tests positive for oxidase and catalase. The bacterium grows at a PH range of 5.5 to 9.0 (optimally 7 to 7.5) and a temperature of 15-42 °C (optimally at 30-37 °C). Through phylogenetic analysis, the species was shown to be affiliated to the genus Ideonella, and also related to Ideonella dechloratans and Ideonella azotifigens.


The site below is methods on how a newly found microorganism is classified according to their domain, phylum, class, family, genus and species.

hope you guys can take a look and learn something from it. 
Thank you.


]]> 2017-04-02 03:46:32 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164138931 lucy8496 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164159015 A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterium, designed strain 201-F6T, was isolated from a microbial consortium that degrades poly(ethylene terephthalate) (PET) collected in Sakai city, Japan, and was characterized on the basis of a polyphasic taxonomic study. The cells were motile with a polar flagellum. The strain contained cytochrome oxidase and catalase. It grew within the pH range 5.5–9.0 (optimally at pH 7–7.5) and at 15–42 ºC (optimally at 30–37 ºC). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). C_{16 : 0}, C_{1 7 : 0} cyclo, C_{18 :1}ω7c and C_{12 : 0} 2-OH were the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of genomic DNA was 70.4 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that strain 201-F6T was affiliated to the genus Ideonella , and was closely related to Ideonella dechloratans LMG 28178T (97.7 %) and Ideonella azotifigens JCM 15503T (96.6 %). Strain 201-F6T could be clearly distinguished from the related species of the genus Ideonella by its physiological and biochemical characteristics as well as by its phylogenetic position and DNA–DNA relatedness. Therefore, the strain represents a novel species of the genus Ideonella , for which the name Ideonella sakaiensis sp. nov. (type strain 201-F6T=NBRC 110686T=TISTR 2288T) is proposed.

Abbreviation:
PET | poly(ethylene terephthalate)

]]> 2017-04-02 12:44:24 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164159015 eden_haziq97 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164521235 Hi guys, today I would like to share about you guys some information about the ecological and systematic about the microbe Ideonella sakaiensis. This microbe is actually still new to the world. So scientists still doing research upon this microbe. But these are some information that scientist have been discovered. Hope this was helpful :)]]> 2017-04-04 05:03:02 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/164521235 lksoon94 https://padlet.com/anisz2508/pv99qo4ceg6w/wish/189640354 2017-09-21 08:06:02 UTC https://padlet.com/anisz2508/pv99qo4ceg6w/wish/189640354