https://padlet.com/amendoza2/po5826cu8d6 By: Jayla Hines, Alexis Mendoza, Olivia Pope, Allison Solis en-us 2016-03-07 02:47:14 UTC 2025-12-29 06:49:31 UTC hello@padlet.com https://c1.staticflickr.com/5/4020/4421477159_ebff66fb21_b.jpg amendoza2 https://padlet.com/amendoza2/po5826cu8d6/wish/99557791 Can the insertion of the GFP gene from bioluminescent jellyfish into e. coli change/transform e. coli into a bioluminescent form/phenotype by expression of the GFP gene into the green fluorescent protein that glows green?]]> 2016-03-08 02:57:21 UTC https://padlet.com/amendoza2/po5826cu8d6/wish/99557791 amendoza2 https://padlet.com/amendoza2/po5826cu8d6/wish/99557809 The Green Flourescent Protein that makes jellyfish glow in the dark transforms the e.coli into a biolumuinescent phenotype, which causes the bacteria to glow the unique bright green color under ultraviolet light. ]]> 2016-03-08 02:57:37 UTC https://padlet.com/amendoza2/po5826cu8d6/wish/99557809 amendoza2 https://padlet.com/amendoza2/po5826cu8d6/wish/99557819 Although the agar plate was on the bottom of the container, when observations were made the pGLO was found on the top cover of the container. Only one colony of pGLO glowed. According to the observations, only one colony of pGLO glowed, thus leading to finding the transformation efficiency. Since there was only one colony, the transformation efficiency isn't going to be a high number. The transformation efficiency was 6.375 transformants/ug. ]]> 2016-03-08 02:57:45 UTC https://padlet.com/amendoza2/po5826cu8d6/wish/99557819 amendoza2 https://padlet.com/amendoza2/po5826cu8d6/wish/99557836 This can be justified by  stating that the E.coli takes in the pGLO gene during transformation. The transformed E. coli will only grow in the ampicillin and arabinose plates. The pGLO plasmid contains the ampicillin resistant gene and the GFP for glow. ]]> 2016-03-08 02:57:55 UTC https://padlet.com/amendoza2/po5826cu8d6/wish/99557836 opope https://padlet.com/amendoza2/po5826cu8d6/wish/99570240 2016-03-08 06:10:16 UTC https://padlet.com/amendoza2/po5826cu8d6/wish/99570240 asolis2 https://padlet.com/amendoza2/po5826cu8d6/wish/99572785 Number of colonies on LB/amp/ara plate= 1

Micrograms of pGLO DNA spread on the plates = 8/51 or 0.16

]]> 2016-03-08 06:57:36 UTC https://padlet.com/amendoza2/po5826cu8d6/wish/99572785