CYTOMEGALOVIRUS (CMV) by Nurul Husna https://padlet.com/nhusspil98/p4xfq2atwrz8 Human cytomegalovirus en-us 2017-12-04 01:39:16 UTC 2023-02-28 10:37:22 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/Dootheets.png Journal article syfkhamrdn https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212687960 2017-12-04 01:46:49 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212687960 CMV Testing faina_ https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689173 * Cytomegalovirus (CMV) testing is used to determine whether someone with signs and symptoms has an active CMV infection. *Sometimes it may be ordered to help determine whether someone had a prior CMV infection.               
* The choice of tests and samples collected depends on the age of the person, their general health status and symptoms, and on the health practitioner's clinical findings and suspicions of organ involvement.
* antibody testing is done on blood sample - two antibodies; IgM & IgG
*antibody testing also used to determine immunity to primary CMV infections.                             

]]> 2017-12-04 01:56:54 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689173 nhusspil98 https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689199 2017-12-04 01:57:14 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689199 ELISA syoga_let https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689471 The enzyme-linked immunosorbent assay (ELISA) is the most common serologic test for measuring antibody to CMV.

]]> 2017-12-04 02:00:01 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689471 nhusspil98 https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689548  

Cytomegalovirus (CMV) infection facts

  • CMV is a common virus in the same family as herpesvirus, and it can infect anyone.
  • CMV is spread by direct contact of body fluids, such as saliva, blood, urine, semen, vaginal fluids, and breast milk. Thus breastfeeding, blood transfusions, organ transplants, and sexual contact are possible modes of transmission.
  • Most healthy people do not experience any symptoms when infected with CMV, and it does not pose a serious health concern. A majority of adults have antibodies consistent with past infection.
  • Most healthy children and adults who do have symptoms will recover from CMV infection without complications and do not require antiviral treatment.
  • However, in those with a weakened immune system, CMV can cause serious disease (retinitis, hepatitis, colitis, pneumonia, or encephalitis).
  • Infants born to mothers infected with CMV during pregnancy may develop congenital CMV infection.
  • Health-care professionals diagnose CMV infections by culturing the virus, detecting CMV DNA from the infected individual, or detecting CMV antibodies.
  • Antiviral treatments may improve the prognosis in some patients with cytomegalovirus infections.
  • There is no commercially available CMV vaccine. Experimental vaccines are being studied. 
https://www.medicinenet.com/cytomegalovirus_cmv/article.htm
]]> 2017-12-04 02:00:52 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689548 nhusspil98 https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689675 Virus classification
Group: | Group I (dsDNA)
Order: | Herpesvirales
Family: | Herpesviridae
Subfamily: | Betaherpesvirinae
Genus: | Cytomegalovirus
Type species
Human cytomegalovirus]]> 2017-12-04 02:01:50 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689675 PCR -polymerase chain reaction syoga_let https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689738 The standard laboratory test for diagnosing congenital CMV infection is PCR on saliva, with urine usually collected and tested for confirmation. 
  • because most CMV seropositive mothers shed CMV virus in their breast milk. 
  • cause a false-positive CMV result on saliva collected shortly after the baby has breast fed.
Specific steps for appropriate collection of saliva samples from a baby :

  1. Saliva collected more than one hour after breastfeeding and within 3 weeks of birth - because detection of CMV after 3 weeks could be the result of post-partum infection.
  2.  a sterile cotton or polyester swab insert into the baby’s mouth between the gum and cheek and swirl for several seconds.
  3. Remove the swab and place into buffer formulated for PCR diagnostic testing . If CMV is present, it will leach from swab to the liquid.
  4. The liquid is processed according to manufacturer’s instructions, and PCR testing is performed according to the protocol in the laboratory.
  5. Specific procedures and interpretation of tests vary according to laboratory.
]]> 2017-12-04 02:02:35 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689738 Methods - Antibody testing syfkhamrdn https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689946 CMV IgM detection. CMV infection was diagnosed by the detection of CMV-specific IgM in serum samples (Eurogenetics CMV IgM ELISA assay). Patients were only included in the study if they had CMV-specific IgM levels of >300 U/mL in the absence of Epstein-Barr virus, hepatitis A, antibody to hepatitis B core antigen, and Toxoplasma gondii IgM antibodies and of rheumatoid factor.

CMV IgG avidity. The specificity of our CMV IgM assay was verified by performing CMV IgG avidity tests on all available samples from the study by using a modified CMV IgG EIA (Biokit) and 8M urea.
]]> 2017-12-04 02:04:43 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689946 nhusspil98 https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689975 2017-12-04 02:05:00 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212689975 nhusspil98 https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690141 Viruses in Cytomegalovirus are enveloped, with icosahedral, Spherical to pleomorphic, and Round geometries, and T=16 symmetry. The diameter is around 150–200 nm. Genomes are linear and non-segmented, around 200kb in length]]> 2017-12-04 02:06:37 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690141 Introduction syfkhamrdn https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690472 Cytomegalovirus (CMV) (from the Greek cyto-, "cell", and megalo-, "large") is a genus of viruses in the order Herpesvirales, in the family (150-200nm in diameter [hiv virus 120nm]) Herpesviridae, in the subfamily Betaherpesvirinae. Humans and monkeys serve as natural hosts.

There are currently eight species in this genus including the type species, human cytomegalovirus (HCMV, human herpesvirus 5, HHV-5), which is the species that infects humans.

Diseases associated with HHV-5 include glandular fever, and pneumonia.[2][3] In the medical literature, most mentions of CMV without further specification refer implicitly to human CMV. Human CMV is the most studied of all cytomegaloviruses ]]> 2017-12-04 02:09:43 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690472 How You Can Get It faina_ https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690479 you probably can catch CMV  by touching your eyes, mouth or nose after contact with infected person through 
*saliva 
*semen
*vaginal fluids 
*blood 
*urine
*breat milk 
*organ transplants]]> 2017-12-04 02:09:45 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690479 nhusspil98 https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690795 2017-12-04 02:12:29 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212690795 Preventing CMV infection syfkhamrdn https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212691164  The following precautions may help lower the risk of contracting CMV: 
  • Hand washing regularly with soap and water
  • Avoid kissing a young child, including tear and saliva contact
  • Avoid sharing glasses and kitchen utensils, for example, passing round a drink
  • Dispose of diapers, paper handkerchiefs and so on carefully
  • Use a condom to prevent the spread of CMV via vaginal fluids and semen.
 The CDC urges parents and caregivers of children with CMV to seek treatment as early as possible, whether it is medication or attending services such as hearing checks. 
]]> 2017-12-04 02:15:52 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212691164 Who is at risk? syoga_let https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212691604
  • Newborn babies
  •  pregnant women
  • people receiving an organ transplant
  •  people with a weakened immune system 
    • ]]>     2017-12-04 02:18:30 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212691604     syoga_let https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212691873
    wash hands with soap and warm water:
    • before preparing, serving or eating food
    • after going to the toilet
    • after changing a baby’s nappy
    • after coming into contact with bodily fluids, such as semen or urine
    ]]>     2017-12-04 02:20:42 UTC https://padlet.com/nhusspil98/p4xfq2atwrz8/wish/212691873