https://padlet.com/amayes81/oez1m64cwm44w5iu en-us 2023-01-31 21:14:19 UTC 2023-01-31 21:53:05 UTC hello@padlet.com amayes81 https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463715348 Get all of your sterilized equipment together and sterilize the hood. Make sure your culture has not been tainted or ruined.]]> 2023-01-31 21:35:04 UTC https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463715348 amayes81 https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463719462 ADHERENT CELLS:
1) Remove the medium with a pipette to rinse cells with a salt solution (no magnesium or calcium)
2) Remove the salt solution and add a dissociation reagent to lift the cells off the plate without harming them
3) Quickly make sure the cells lifted off so you can remove the reagent
4) Break up the clumps by rinsing with a warm medium to aid in accurate counting
5) Place cells into the centrifuge to get rid of any leftover dissociation reagent and remove the medium from the tube
SUSPENSION CELLS:
1) Remove a sample from the cell culture

]]> 2023-01-31 21:40:21 UTC https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463719462 amayes81 https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463720697 Remove a sample to stain with Trypan blue for counting of the ratio of live:dead cells.]]> 2023-01-31 21:41:55 UTC https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463720697 amayes81 https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463722045 Add a properly calculated amount of medium for your cells and mix. Seal them in a capped flask. You may need more than one flask depending on your max and min volume.]]> 2023-01-31 21:43:48 UTC https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463722045 amayes81 https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463723168 Evenly distribute your cells and put them in the incubator. Make sure to check them every 3 weeks to gently spin the cells, remove and replace the medium so there is no cell debris or waste. ]]> 2023-01-31 21:45:20 UTC https://padlet.com/amayes81/oez1m64cwm44w5iu/wish/2463723168