Controlling blood pressure. 
Soy beans in the tempe is the source of iron(Fe), calcium(Ca) and pottasium(K). those element plays important role in controlling blood pressure. High potassium levels is what that makes soy is very useful to prevent and control the rise in blood pressure. 

 Prevent Cancer 
The fiber content in tempe is not only effective for improving the performance of the gastrointestinal tract, but also effective in binding toxic and cancer-causing cholesterol and remove it from the body. The bound toxins cannot damage the cells in the body. 

 Prevent anemia 
Tempe can help to prevent anemia. It is because of the high content of various minerals that are easily absorbed by blood and prevent osteoporosis. 

2.Explain three (3) advantages of "tempe" to health
 

Tempeh is high in soy protein, which can promote satiety, reduce hunger and increase weight loss.

- Reduces cholesterol level
Tempeh is traditionally made from soybeans, which contain natural plant compounds called isoflavones. Studies show that soy isoflavones and soy protein may decrease blood cholesterol levels.

- Keeping bone strong 
Tempeh is high in calcium and may help increase bone density and prevent bone loss.

Source of vitamins (especially vitamin B) is very useful for:

~Controlling Blood Sugar

Tempe is also safe for diabetics. The content of protein and fiber contained in tempe was able to prevent the rise in blood sugar levels. Diabetics are usually more at risk of developing atherosclerosis or vascular inflammation associated with heart disease, so it should keep blood cholesterol levels remain low. This is why eating tempe is good for diabetics.

~Prevent anemia

Tempe can prevent anemia. The high content of various minerals in tempe that are easily absorbed by blood and prevent osteoporosis.

-vitamin B12 can found in animal food only but the tempe is the only vegetable food which contain vitamin B12

- Neutralize the negative effects of bad cholesterol because it contains polyunsaturated fat (PUFA), niacin, omega 3 and 6 so as to reduce the risk of heart attack (coronary) 

-Tempe is useful as free-radical scavengers degenerative diseases prevention (atherosclerosis, coronary heart disease, diabetes mellitus, hypertension, and others) and various types of cancer (pancreatic, prostate and breast) and prevent premature aging because it contains powerful antioxidants (three types of isoflavones). 

Neutralize the negative effects of bad cholesterol because it contains polyunsaturated fat (PUFA), niacin, omega 3 and 6 so as to reduce the risk of heart attack (coronary).

Prevent anemia

Tempe can help to prevent anemia. It is because of the high content of various minerals that are easily absorbed by blood and prevent osteoporosis 

Prevent Premature Aging Process

Free radicals are organic molecules responsible for aging, tissue damage, and the possibility of some diseases such as cancer and heart disease. 

The fiber content in tempe is not only effective for improving the performance of the gastrointestinal tract, but also effective in binding toxic and cancer-causing cholesterol and remove it from the body. The bound toxins can not damage the cells in the body. Research conducted at the University of North Carolina, found that genestein and phytoestrogens in soybean was able to prevent prostate and breast cancer, and premature aging

ii) Reduce the risk of heart disease

Neutralize the negative effects of bad cholesterol because it contains polyunsaturated fat (PUFA), niacin, omega 3 and 6 so as to reduce the risk of heart attack (coronary). 

iii) Controlling Blood Sugar

Tempe is also safe for diabetics. The content of protein and fiber contained in tempe was able to prevent the rise in blood sugar levels. Diabetics are usually more at risk of developing atherosclerosis or vascular inflammation associated with heart disease, so it should keep blood cholesterol levels remain low. This is why eating tempe is good for diabetics. 

 

1)       Reduce cholesterol level 

Tempeh is traditionally made from soybeans, which contain natural plant compounds called isoflavones.Soy isoflavones have been associated with reduced cholesterol levels. 

2)      High in protein 

Tempeh is high in protein. One cup (166 grams) provides 31 grams of protein. Some studies suggest that a protein-rich diet may stimulate thermogenesis, leading to an increase in metabolism and helping your body burn more calories after each meal 

3)      Contain probiotics 

Fermentation is a process that involves the breaking down of sugars by bacteria and yeast. Through fermentation, the phytic acid found in soybeans is broken down, helping to improve digestion and absorption 

 

The consumption of fermented, probiotics foods has many benefits. The microflora that lives in fermented foods creates a protective lining in the intestines and shields it against pathogenic factors such as salmonella and E.coli

The calcium provided by tempe is involved in the growth and maintenance of bones. Calcium is needed to maintain bone mineral density and prevent weak, brittle bones and fractures. Copper, another mineral present in tempe also plays an important role in growing bones.

In addition to supporting human immunity to disease, research has shown that probiotics can improve digestive function, aid mineral absorption and heal leaky gut syndrome that contributing to cancer prevention.

      Improve Immune System and Health 

Amino acids can enhance the benefits of Tempe in boosting the immune system. Tempe is also an important source of vitamin B12, which vegetarians can use to replace meat, and an excellent source of calcium
      High Fiber 

High content of soybean fiber are found in Tempe can help patients with diabetes to control their blood sugar levels. It can also prevent colon cancer, improving kidney health by replacing animal protein with soy, and prevent diarrhea in children.
  Controlling blood pressure 

Soy is a source of iron (Fe), calcium (Ca) and potassium (K). Potassium absorption of soy beans is relatively better than from any other vegetable food ingredient. High potassium levels is what that makes soy is very useful to prevent and control the rise in blood pressure. 

1. List four (4) enzyme extraction methods. 

·         Isolation and purification 

·         Filtration 

·         Ultrasonification 

·         Centrifugation 

 

2.Explain three (3) advantages of “tempeh” to health 

 

A. Anticancer Effect. 

 

-Fermentation bacteria produce short chain fatty acids which can prevent breast cancer and produce anti-mutagenic organic acid to normalize the intestinal permeability. 

 

B. Reduces Menopausal Symptoms. 

 

-During menopause, estrogens level of body starts decreasing. Isoflavones in soy works as estrogens to helps reduce menopausal symptoms, especially hot flushes. 

 

C. Good for diabetic patient. 

 

-The protein in tempeh is excellent for diabetic patients, who tend to have problems with animal sources of protein. The protein and fiber in tempeh can also prevent high blood sugar levels and help in keeping blood sugar levels under control. 

- Purification
- Centrifugation
- Ultrasonification

2. Three advantages of “tempe” to health :

Neutralize the negative effects of bad cholesterol because it contains polyunsaturated fat (PUFA), niacin, omega 3 and 6 so as to reduce the risk of heart attack (coronary).

Tempe can help to prevent anemia. It is because of the high content of various minerals that are easily absorbed by blood and prevent osteoporosis.

Soybeans in fact contain anti-cancer compounds. One risk factor for breast cancer is high levels of estrogen in the blood (postmenopausal androgen). High levels of estrogen can be lowered by consuming food that is high fiber content so that the majority of estrogen that can be excreted out of the body.

1. Four Enzyme extraction method are :

-Filtration 

-Purification 

-Centrifugation        

-Ultrasonification 

 2. Three advantages of tempe to health 

Increase bone density
Calcium, together with minerals like vitamin K and vitamin D, is needed to maintain bone    
mineral density and prevent weak, brittle bones and fractures as it involved in the growth and maintainance of bones. 

reduce the risk of heart disease
Neutralize the negative effects of bad cholesterol because it contais polyunsaturated fat (PUFA),   
niacin, omega 3 and so as to reduce the risk of heart attack (coronary)

Breast cancer prevention
One risk factor for breast cancer is high levels of estrogen in the blood (postmenopausal androgen). High levels of estrogen can be lowered by consuming food that high fiber content o that the majority of estrogen can be exctreted out of the body. 

1. List four (4) enzyme extraction methods.

- Purification
- Centrifugation
- Filtration
- Ultrasonification

2. Explain three (3) advantages of “tempe” to health.

I. Prevent anaemia

Tempe can help to prevent anaemia. It is because of the high content of various minerals that are easily absorbed by blood and prevent osteoporosis.

II. Prevent Cancer

The fibre content in tempe is not only effective for improving the performance of the gastrointestinal tract, but also effective in binding toxic and cancer-causing cholesterol and remove it from the body. The bound toxins cannot damage the cells in the body. Research conducted at the University of North Carolina, found that gene stein and phytoestrogens in soybean was able to prevent prostate and breast cancer, and premature aging.

III. Controlling Blood Sugar

Tempe is also safe for diabetics. The content of protein and fibre contained in tempe was able to prevent the rise in blood sugar levels. Diabetics are usually more at risk of developing atherosclerosis or vascular inflammation associated with heart disease, so it should keep blood cholesterol levels remain low. This is why eating tempe is good for diabetics.

Four (4) enzyme extraction methods:
1. Filtration
2. Purification 
3. Centrifugation
4. Ultrasonication

Three (3) advantages of “tempe” to health:

1. Tempeh can reduces cholestrol

Rhizopus moulds produce natural, heat-stable antibiotic agents against some disease-causing organisms. Indonesians who eat tempeh as a regular part of their diet recognize it as a medicine for dysentery and rarely fall victim to the intestinal diseases to which they are constantly exposed. 

One serving of tempeh contains more fiber than most peoples consume in one day. Fiber is essential for a healthy digestive tract as well as preventing many chronic diseases.

Tempeh is a great choice for people who have difficulty digesting plant-based high-protein foods like beans and legumes or soy foods such as tofu. The process of fermentation makes the soybeans softer, since enzymes produced by the mould predigests a large portion of the basic nutrients. The Rhizopus moulds produce an enzyme phytase which breaks down phytates, thereby increasing the absorption of minerals such as zinc, iron and calcium. The fermentation process greatly reduces the oligosaccharides that make beans hard to digest for some people. Studies have shown tempeh to be essentially non-flatulent and producing no more gas than non-legume food. 

1) List 4 enzyme extraction methods .
~filtration
~purification
~centrifugation
~ultrasonication

2. Explain three advantages of tempe to health

Good for diabetes
Protein in tempe is good for diabetic patients who often have problems with animal protein sources. Protein and fiber in tempe can also prevent the rise in blood sugar and keep blood sugar levels under control.

Prevent anemia
Tempe can help to prevent anemia. It is because of the high content of various minerals that are easily absorbed by blood and prevent osteoporosis

Contains a natural antibiotic

Rhizopus fungi produce natural antibiotic substances to against some harmful organisms. Natural antibiotic substances in Tempe this could be a cure for dysentery when consumed every day.

1.Source of good protein 

-Protein keep our metabolism running, our energy up and our blood sugar levels stable. The high level of protein are critical for building muscle mass, supporting neurological function, aiding in digestion, balancing hormones and keeping an upbeat mood. 
 

2. Have a number of active substances 

-Tempe contain soy isoflavone which their consumption may exert cancer-preventive effects by decreasing estrogen synthesis and altering metabolism away from gene-damaging metabolites toward inactive metabolites. 

3.  Sources of essential fatty acid 

- Functions of essential fatty acids include improving immunity, cell signaling, mood and brain health, plus decreasing inflammation. It also enhance mental and physical performance, help treat some diseases, promote mental health, and improve body composition. 

1.      List 4 enzymes extraction methods 

       i.          Filtration 

      ii.          Purification 

     iii.          Centrifugation 

     iv.          Ultrasonication 

2.      Explain 3 advantages of ‘tempe’ to health 

       i.          Help to lower cholesterol level 

-        ‘Tempe’ is high in fiber content. It contains the powerful isoflavones and Vitamin B3 which function in lowering the cholesterol level. 

      ii.          Help to prevent diabetes 

-        ‘Tempe’ consist of manganese which is an essential mineral in reducing the risk of having diabetes. Manganese helps to increase the amount of digestive enzymes produced during the digestive process which aid in the nutrient absorption. It also limits the rate of carbohydrates and sugar being absorbed into the blood. 

    iii.          Help to reduce muscle spasms 

-        ‘Tempe’ consist of potassium and magnesium which aid in the reducing of muscle cramps, tension and fatigue. The blood circulation can also be improved to help the muscle recovers faster after a workout. 

2. Explain 3 advantages of tempeh to health

1.Prevent anemia

 Tempe can help to prevent anemia. It is because of the high content of various minerals that are easily absorbed by blood and prevent osteoporosis
  
2. Helps the metabolism of red blood cell
Source of vitamin especially vitamin B is very useful for red blood cell metabolism,healthy skin and muscle,improve the immune and nervous system function.

3. Increase bone density
 The calcium provided by tempeh is involved in the growth and maintenance of bones.calcium is needed to maintain bone mineral density and prevent weak,brittle bones and fractures.copper another mineral that present in tempeh.

Explanation: Tempeh is high in protein which is good for a diebetic patients who they are often have problems with animal protein sources. They also prevent the rise in blood sugar and will keep blood sugar under control.

2) Reduce cholesterol

Explanation: Niacin in tempeh is considered an important treatment option for helping reduce dangerously high cholesterol levels. This help to lower the risk of cardiovascular disease in patients that mixed with dyslipidemia.(Dyslipidemia is an elevation of plasma cholesterol, triglycerides or both.

3) Help to reduce muscle spasms

Explanation: Tempeh contain potassium and magnesium that help in reducing of muscle cramps, tension and fatigue. Blood circulation helps the muscle recovers faster after doing a workout.

Rhizopus fungus will produce antibiotic subtances that against a number of harmful organisms. 

 
   - Sources of Vitamin B (B12)

Tempe is a very good source of vitamin B. Even tempe is the only source of vitamin B12 from food vegetable (usually vitamin B12 is only contained in animal food). Because of that vegetarians prefers to make tempe instead of meat. Other vitamins contained in Tempe is vitamin B2 (riboflavin), B6 (pyridoxine), B1 (thiamin), niacin, folic acid, and pantothenic acid.

    - Healthy for Heart

In recent years, soy proteins have become a new icon in maintaining heart health. Research has also shown that the protein content in tempe can reduce bad cholesterol by 30-45 percent.As we know that the bad cholesterol (LDL) is the causative factor blockage of blood vessels that can lead to heart attacks and strokes. Research also indicates that tempe may increase the levels of HDL, or good cholesterol in the blood, which is useful to suppress the amount of bad cholesterol and remove it from the body.

 

Tempeh is traditionally made from soybeans, which contain natural plant compounds called isoflavones.
Soy isoflavones have been associated with reduced cholesterol levels.

+ Promote Bone Health

Tempeh is a good source of calcium, a mineral that is responsible for keep bone strong and dense.
Adequate calcium intake may prevent the development of osteoporosis, a condition that is associated with bone loss and porous bones.

+ Prevent Premature Aging Process

Free radicals are organic molecules responsible for aging, tissue damage, and the possibility of some diseases such as cancer and heart disease.

-Purification 

-Centrifugation        

-Ultrasonification 

 2. Three advantages of tempe to health
-Help to prevent cancer
The bound toxins can not damage the cells in the body. The fiber content is effective in binding toxic and remove it from the body. It prevent cancer causing. The genestein and phytoestrogens in soybean was able to prevent prostate and breast cancer, and premature aging.
-Improve Immune System and Health 

Amino acids can enhance the benefits of Tempe in boosting the immune system. Tempe is also an important source of vitamin B12, which vegetarians can use to replace meat, and an excellent source of calcium.
-Help to prevent diabetes 
‘Tempe’ consist of manganese which is an essential mineral in reducing the risk of having diabetes. Manganese helps to increase the amount of digestive enzymes produced during the digestive process which aid in the nutrient absorption. It also limits the rate of carbohydrates and sugar being absorbed into the blood. 

Neutralize the negative effects of bad cholesterol because it contains polyunsaturated fat (PUFA), niacin, omega 3 and 6 so as to reduce the risk of heart attack (coronary).

The calcium provided by tempeh is involved in the growth and maintenance of bones. Calcium, together with the other essential minerals like vitamin K and vitamin D, is needed to maintain the bone mineral density and prevent weak, brittle bones and fractures. It helps form a part of hydroxyapatite, the mineral complex that makes bones and teeth hard, this complex also maintains bone density and helps bones heal.

The niacin in tempeh is considered an important treatment option for helping reduce dangerously high cholesterol levels. Niacin, or vitamin B3, has been proven to lower the risk of cardiovascular disease in patients with mixed dyslipidemia.  Dyslipidemia is an elevation of plasma cholesterol, triglycerides or both. 

The fiber content in tempe is not only effective for improving the performance of the gastrointestinal tract, but also effective in binding toxic and cancer-causing cholesterol and remove it from the body. The bound toxins cannot damage the cells in the body. 

Tempe consist of manganese which is an essential mineral in reducing the risk of having diabetes. Manganese helps to increase the amount of digestive enzymes produced during the digestive process which aid in the nutrient absorption. It also limits the rate of carbohydrates and sugar being absorbed into the blood.

c) Help in reducing the risk of heart disease
Neutralize the negative effects of bad cholesterol because it contains polyunsaturated fat (PUFA), niacin, omega 3 and 6 hence reducing the risk of heart attack (coronary). 

-Filtration 

-Purification 

-Centrifugation        

-Ultrasonification 

2.Explain three (3) advantages of “tempe” to health. 

1). Fermentation is a process that involves the breaking down of sugars by bacteria and yeast. Through fermentation, the phytic acid found in soybeans is broken down, helping to improve digestion and absorption 

ote Bone Health

2) Tempeh is a good source of calcium, a mineral that is responsible for keep bone strong and dense.
Adequate calcium intake may prevent the development of osteoporosis, a condition that is associated with bone loss and porous bones.

3)   High content of soybean fiber are found in Tempe can help patients with diabetes to control their blood sugar levels. It can also prevent colon cancer, improving kidney health by replacing animal protein with soy, and prevent diarrhea in children. 

Neutralize the negative effects of bad cholesterol because it contains polyunsaturated fat (PUFA), niacin, omega 3 and 6 so as to reduce the risk of heart attack (coronary).

     b)Controlling Blood Sugar

Tempe is also safe for diabetics. The content of protein and fiber contained in tempe was able to prevent the rise in blood sugar levels. Diabetics are usually more at risk of developing atherosclerosis or vascular inflammation associated with heart disease, so it should keep blood cholesterol levels remain low. This is why eating tempe is good for diabetics.

     c)Anti-free radicals

Tempe is useful as free-radical scavengers degenerative diseases prevention (atherosclerosis, coronary heart disease, diabetes mellitus, hypertension, and others) and various types of cancer (pancreatic, prostate and breast) and prevent premature aging because it contains powerful antioxidants (three types of isoflavones).

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1. 4 enzymes extraction methods are:
-filtration
-centrifugation
-purification
-ultrasonification

2. 3 advantages of tempe to health are:
1. Helps Lower Blood Pressure
Tempe contains magnesium, which this substance plays an important role on the cardiovascular system and is responsible for more than 300 metabolic processes in the body. Magnesium is beneficial for preventing blood clots, lower blood pressure, maintaining bone strength, and help prevent complications associated with diabetes.

2. High Fiber

High content of soybean fiber are found in Tempe can help patients with diabetes to control their blood sugar levels. It can also prevent colon cancer, improving kidney health by replacing animal protein with soy, and prevent diarrhea in children.

3. Sources of Energy
Tempe also contains riboflavin, known as vitamin B2. These micronutrients are easily digested and water – soluble nature . Riboflavin has an important role in energy metabolism and is also needed in the metabolism of carbohydrates, protein, fat and ketone substances. In addition, this vitamin plays a role in the formation of red blood cells. Riboflavin deficiency affects the body’s energy production . Early symptoms of riboflavin deficiency are usually chapped lips and sore throat.

Tempeh contains high-quality complete protein and provides all the amino acids that needed in our body. Protein help to maintain muscle tissue. The protein in tempeh is more digestible as compared to animal sources.
b) Prevention of type 2 diabetes.
Soy food like "tempeh" have proven with the ability to lessen insulin resistance by increasing the synthesis of insulin receptors.
c) Cancer prevention benefits.        The soybean in "tempeh" consists lignan phytoestrogens that can  help prevent some forms of cancer by blocking estrogenic activity, reducing the risk of breast, prostate and colon cancers, along with cardiovascular disease. High content  of soy isoflavone (Genistein) in "tempeh" can increase activity of a tumor suppressor protein called p53. As p53 becomes more active, it can help trigger programmed cell death (apoptosis) in cancer cells, and it also help trigger cell cycle arrest (helping stop ongoing cancer cell activity). Genistein are potentially in  blocking the activity of protein kinases  that  slow down the tumor formation, especially in the case of breast and prostate cancer.