https://padlet.com/mms2012171240/nel9gj7ceqenzphv GROUP 6 en-us 2021-11-05 14:24:47 UTC 2021-11-07 16:51:16 UTC hello@padlet.com http://yessicaponcebiotech.weebly.com/uploads/2/3/4/3/23430374/6569724_orig.jpg mms2012171240 https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1870105962 a) The DNA that contain genes from different sources that have been combined by the techniques of genetic engineering.
b) Source of plasmid = bacterial cell
     Role = Carries the foreign DNA fragments to be introduced into bacterial cell
c) An endonuclease that recognize and cleave DNA molecule at specific nucleotide sequences (restriction sites). 
d) Restriction site = 5' GGATCC 3'
     Restriction enzyme = Bam HI
e) i) 2
ii) 2
f) Modifying enzyme
g) Because bacteria can reproduce faster.

]]> 2021-11-05 14:30:22 UTC https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1870105962 mms2012171240 https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1870161911 a) Q
b) -able to accept the foreign DNA in multiple cloning sites.
    -present of origin of replication to be able to replicate freely in the host cell.
    -has selectable marker gene for screening.
c) Recombinant plasmid
d) Host cell
e) -able to receive recombinant plasmid through transformation process.
    -able to maintain the structure of the recombinant plasmid from one generation to another generation.
f) Impossible to use different type of S because it will not produce compatible sticky ends on both the foreign DNA fragments and the plasmid.]]> 2021-11-05 14:53:39 UTC https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1870161911 https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1872215368 a) One difference between EcoRI and SmaI are, EcoRI produce compatible sticky end while SmaI produce compatible blunt end after cleaved with the same restriction enzyme. Another difference is EcoRI leaves a staggered cut with single-stranded ends while SmaI makes a simple cut across both strands at a single point.

b) i) Sticky end is when Restriction enzyme cleave the sugar-phosphate backbones in the two DNA strand in a staggered cut. Resulting double stranded restriction fragments have at least one single-stranded end.
    
     ii) Palindromic sequence
         5'    GAATTC   3'
         3'    CTTAAG   5'
  
    iii) 2 DNA fragment are produced.

]]> 2021-11-07 03:36:26 UTC https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1872215368 https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1872373857 a) The transformed bacteria cell will die. It is because it does not have tetracycline resistant gene so it cannot resist to tetracycline.

b) The host cell will survive.]]> 2021-11-07 07:43:13 UTC https://padlet.com/mms2012171240/nel9gj7ceqenzphv/wish/1872373857