Técnicas de extracción de Ácidos nucleicos by janet renteria https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp Materia: Genética Subtema: extracción de ADN en-us 2024-05-31 22:00:07 UTC 2024-06-01 07:14:18 UTC hello@padlet.com Introducción al Método Fenol-Cloroformo renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725516 2024-05-31 22:00:08 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725516 Procedimiento Paso a Paso renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725524 2. Fenol: Agregar fenol para separar las proteínas del ADN.
3. Cloroformo: Añadir cloroformo para purificar el ADN.
4. Centrifugación: Separar fases acuosa y orgánica.
5. Precipitación: Usar alcohol frío para precipitar el ADN.
6. Recolección: Recuperar el ADN precipitado.]]> 2024-05-31 22:00:10 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725524 Resultados Esperados renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725525 2024-05-31 22:00:10 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725525 Introducción al Método Salting Out renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725529 2024-05-31 22:00:10 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725529 Instrucciones Detalladas renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725530 2. Salting Out: Agregar solución saturada de sal para precipitar proteínas.
3. Centrifugación: Realizar centrifugación para separar el ADN de las proteínas precipitadas.
4. Precipitación: Utilizar alcohol isopropílico frío para precipitar el ADN.
5. Recolección: Recuperar el ADN precipitado para su análisis.]]> 2024-05-31 22:00:11 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725530 Análisis de Resultados renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725531 2024-05-31 22:00:11 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725531 Uso de Kits Comerciales renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725535 2024-05-31 22:00:12 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725535 Pasos del Proceso renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725552 2. Unión: Aplicar la muestra a la columna de sílice para que el ADN se una.
3. Lavado: Lavar la columna con el buffer de lavado del kit.
4. Elución: Eluir el ADN puro con un buffer adecuado.
5. Cuantificación: Medir la concentración de ADN con un espectrofotómetro.]]> 2024-05-31 22:00:14 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725552 Ventajas y Aplicaciones renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725553 2024-05-31 22:00:14 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014725553 Janet Renteria García renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014726336 2024-05-31 22:02:33 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014726336 Janet Renteria García renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014739727 2024-05-31 22:55:13 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014739727 renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014740923 2024-05-31 23:00:59 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014740923 renteriajanet22 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014846194 2024-06-01 04:40:19 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014846194 Juan Luis Moreno Méndez https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014866670 2024-06-01 05:59:15 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014866670 nitram0512 https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014887744 2024-06-01 07:10:04 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014887744 CYGM https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014888904 2024-06-01 07:14:18 UTC https://padlet.com/renteriajanet22/n8n8fp1yu7mjextp/wish/3014888904