https://padlet.com/amberm101550/n3rzbuhptuykmwt7 Made with a little mischief en-us 2022-03-15 18:03:47 UTC 2022-03-24 19:26:13 UTC hello@padlet.com amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2096558504           Amber Montoya 
               3rd Period 
            March 15 2022]]> 2022-03-15 18:20:25 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2096558504 amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2096562235 DNA, also known as Deoxyribonucleic Acid are two long strands in the form of a double helix that contains our genetic code. Our genetic code are essentially a set of rules used by our cells to convert the instructions in DNA to what we need in life and proteins. DNA is made up of nucleotides that are the building blocks of DNA and made up of phosphates, sugars, and nitrogen bases. These nitrogen bases have rules called Chargaff's rules, where A=T (Adenine = Thymine) and C=G (Cytosine = Guanine).  Connecting these bases are hydrogen bonds. ]]> 2022-03-15 18:22:44 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2096562235 amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2096586005 2022-03-15 18:37:17 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2096586005 amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2102745466 2022-03-19 04:45:24 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2102745466 amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2102746949

GOGGLES

Ziplock bag

A strawberry

10 mL DNA extraction buffer 

Cheesecloth

Funnel

Test tube 

 test tube stand

Stir stick

Ethanol 

Paper towels

]]> 2022-03-19 04:49:02 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2102746949 amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2103330034

Place one strawberry in a Ziploc bag.

Smash/grind up the strawberry using your fist & fingers for 2 minutes. Careful not to break the bag!!

Add the provided 10 mL of extraction buffer (salt & soap solution) to the bag. (DNA Extraction Buffer:1000 ml of deionized water, 50 ml of dish soap, 1 teaspoon of salt)

Gently kneed/mush the strawberry in the bag again for 1 minute.

Assemble your filtration apparatus as shown in the diagram.

Pour the strawberry slurry into the filtration apparatus & let it drip directly into your test tube … only until your test tube is ½ full.

Slowly pour cold ethanol into the tube.

Dip the stir stick into the tube where the strawberry extract and ethanol layers come into contact with each other.

]]> 2022-03-20 01:44:55 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2103330034 amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2103384142 To begin, we put the strawberry in the Ziplock bag and began mush the strawberry for a few minutes to break open the cells. Then we mixed the strawberry mush with salty/soapy solution, mushing it again for 2 minutes to break up proteins and dissolve cell membranes. Continuing that, we then filter strawberry slurry through cheesecloth to separate the components of the cell. Lastly, we pour the mush into the test tube half way then begin to add ethanol to filtered extract to precipitate DNA from the solution. ]]> 2022-03-20 03:59:32 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2103384142 amberm101550 https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2103414443 To sum up our strawberry lab, we crushed the strawberry in a Ziplock bag, added soapy solution and crushed again. Then we added the mushy substance to an apparatus that filtered the strawberry chunks versus the actual juice. With that juice, we added it to a test tube then added the ethanol, where the DNA slowly came up into the ethanol. I learned that plants and veggies, like all other living things, have DNA. If I did this experiment again, I'd probably change out the cheese cloth because it was kind of hard for the mushy juice to separate from the strawberry chunks. Now that I know you can extract DNA from a strawberry, I'd wanna see how the DNA looks under a microscope, whether it looks the same as ours or not. Two traits expressed in a strawberry are color and size. ]]> 2022-03-20 05:19:00 UTC https://padlet.com/amberm101550/n3rzbuhptuykmwt7/wish/2103414443