https://padlet.com/gracieonyia/mypbq7ioxxyy Made with a lightning strike of genius en-us 2018-09-17 20:41:06 UTC 2024-07-11 09:09:58 UTC hello@padlet.com seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/282603828 Life]]> 2018-09-17 20:46:21 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/282603828 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286268420 Food Additives and their effects on the body; whether they actually have negative effects 
-Amy Vo

]]> 2018-09-26 21:08:46 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286268420 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286269721 If we were to test the negative effects of food additives, how would we do so? What materials or substances should we use? 
-Amy Vo

]]> 2018-09-26 21:13:37 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286269721 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286270700 -MSG
-Artificial Food Coloring
-Sodium Nitrite
-high fructose corn syrup
-Carrageenan
-Stevia


-Amy Vo

]]> 2018-09-26 21:17:29 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286270700 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286273975 -Effect of Dopamine on crickets and how their dosages affect the crickets' behaviors
-Amy Vo. ]]> 2018-09-26 21:32:23 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286273975 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286275974 There was a quick article I read that  talked about how Stevia can actually lead to overeating although  it's been deemed to be a lot better than sugar. Is it really all that better though?
-Amy Vo

]]> 2018-09-26 21:40:06 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286275974 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286277307 What's really in milk? ]]> 2018-09-26 21:45:46 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286277307 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286277681 -We can see the effects of certain types of workouts on people (like measuring their blood pressure and heart rate).
-Amy Vo]]> 2018-09-26 21:47:18 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286277681 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286278055 Is milk really good for you? Do we need milk? How is milk negatively affecting health?
-Amy Vo]]> 2018-09-26 21:49:12 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286278055 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286626130 https://www.sciencedirect.com/science/article/pii/S0048969718327669?via%3Dihub
Made me wonder perhaps microplastics are in other organisms we thought were unaffected but actually drastically are? Probably something we might need to go outside for, but just an idea.]]> 2018-09-27 16:36:26 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286626130 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286627166 How detrimental is acid rain to a plant? Could simulate how it affects a plant over time. ]]> 2018-09-27 16:38:01 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286627166 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286631648 https://www.reddit.com/r/science/comments/9jcghk/researchers_connect_lower_antibiotic_resistance/
I know this has been done several times... but perhaps not like this? A different approach with another study's evidence? Also the species of bacteria is pretty interesting, should give it a read.]]> 2018-09-27 16:45:37 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286631648 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286635259 https://www.nature.com/articles/s41598-018-32681-y
Was cool.]]> 2018-09-27 16:51:50 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286635259 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286639846 http://www.ox.ac.uk/news/2018-09-25-addictive-behaviours-have-strong-links-ancient-retroviral-infection
This is interestingggg]]> 2018-09-27 17:00:49 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286639846 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286650239 Cancer cells are easy to obtain, any ideas with such could work and be tested.]]> 2018-09-27 17:20:14 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286650239 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286650632 https://pubs.acs.org/doi/10.1021/acschembio.8b00556

]]> 2018-09-27 17:20:54 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286650632 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286923837 2018-09-28 12:58:11 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286923837 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286946483 We're looking for the effect of prebiotics, milk being one of those, and seeing their effects on probiotics. Could compare respiration rates, prepare a medium, test calcium as an aspect for milk if we wanted?

]]> 2018-09-28 13:40:56 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/286946483 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287270753 We can choose one specific probiotic and test multiple prebiotics on that one probiotic and see the growth rate of the probiotic on the different prebiotics. 
-Amy Vo]]> 2018-09-30 01:46:55 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287270753 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287270861 -Bifidobacteria


-Amy Vo]]> 2018-09-30 01:49:19 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287270861 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287355682 Do can we test the growth and efficiency of different prebiotics (yogurt, kimchi, apple cider vinegar, miso soup).
-Amy Vo]]> 2018-09-30 17:43:42 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287355682 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287362533 2018-09-30 18:30:12 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287362533 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287363189 https://www.eatright.org/food/vitamins-and-supplements/nutrient-rich-foods/prebiotics-and-probiotics-creating-a-healthier-you]]> 2018-09-30 18:33:03 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287363189 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287363270 http://www.navacenter.com/community/article-library/browse/2015/06/01/your-body's-second-brain---the-importance-of-gut-health]]> 2018-09-30 18:33:29 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/287363270 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/288910593 We are testing to see in which conditions Lactobacilli would grow the most in. One prebiotic mixture in agar solution with the lactobacilli  or a mixture with two different prebiotics on the lactobacilli

-Amy Vo

]]> 2018-10-03 22:51:34 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/288910593 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/288910908 Our control would be the test tube that has just the agar solution and the probiotic Lactobacilli itself with no other prebiotics
-Amy Vo]]> 2018-10-03 22:53:54 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/288910908 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289458379 Our negative control would be just the tube with the MRS Agar solution itself
-Amy Vo]]> 2018-10-05 04:52:40 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289458379 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289458442 1) would lactobacilli even grow in the prebiotics in the first place?
2)what concentrations of the prebiotics would we need to put in the test tubes in the first place to induce best growth? 

we need to experiment to answer these two questions first before answering our big question
-Amy Vo]]> 2018-10-05 04:53:21 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289458442 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289915727 Optical density- using Spectrophotometer to absord light, the absorbance is the count of bacteria 

-Amy Vo ]]> 2018-10-06 15:16:05 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289915727 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289915877 We are going to do test runs for the concentrations of prebiotics before doing the 50 test tubes test 
-Amy Vo]]> 2018-10-06 15:17:12 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289915877 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289963406 Inulin and Oligofructose]]> 2018-10-06 23:08:40 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289963406 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289964976 How much Bifidobacteria is one supposed to take in order to produce substantial benefits? What brand/flavor of yogurt should we use? Since there is already bifidobacteria in these food items, how will we ensure accurate results?]]> 2018-10-06 23:38:40 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/289964976 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/290102416 -How much bacteria should we inoculate in the first place and how much of the inoculated bacteria should we us to put into each tube with the prebiotics?
-how many ml of prebiotics should we use to mix into the MRS agar for high, medium, and low concentrations?

]]> 2018-10-08 00:00:42 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/290102416 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298061288 We made the MRS Broth in lab on Monday. We put 55 grams of the broth powder into a beaker with 1000 ml of distilled water. Then we used a stir bar and put the beaker on a heated plate. We then autoclave it with the help of our mentor. 

]]> 2018-10-29 14:58:30 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298061288 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298064358 We haven't gotten our prebiotics yet (inulin and something else that we will have to switch oligofructose out for) so we the MRS broth might not be good to use anymore. 

]]> 2018-10-29 15:03:26 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298064358 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298166256 -scale
-weighting paper 
-stir bar 
-heating plate 
-beaker 
-MRS powder 
-distilled water ]]> 2018-10-29 17:34:40 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298166256 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298269104 https://www.sciencedirect.com/science/article/pii/S0023643812000503]]> 2018-10-29 20:52:55 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298269104 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298274647 For our experiment, we are going to test inulin and an inulin-oligofructose powder on lactobacilli growth rate. ]]> 2018-10-29 21:12:47 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/298274647 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300759554 -We disposed our first batch of MRS broth that we made because the color did not turn out right. It was dark brown when it was supposed to be reddish/orange 

]]> 2018-11-05 22:18:23 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300759554 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300759672 We poured bleach into the MRS broth to get rid of anything hazardous that might have grown in there. It was a precautionary measure. We dumped the mixture into the microbiological waste bin to dispose of it. We put bleach in the empty container and then washed it thoroughly with soap and water. 

]]> 2018-11-05 22:19:01 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300759672 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300763627 We are about to grow the Lactobacilli tomorrow if TA permits. But we only have our inulin, and not the combined powder. So we're just testing our inulin at concentrations of .1, .3, and .5 grams per 5 mL of MRS broth. ]]> 2018-11-05 22:36:12 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300763627 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300764385 If the TA grants us access to the lab, we will be able to start growing the Lactobacilli in inulin on Wednesday, November 7. ]]> 2018-11-05 22:39:53 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/300764385 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/303556424 Today, we finally started to grow the Lactobacilli in the MRS Broth and creating the stock solution. 
First, we autoclaved a 300 mL container, and then we heated up the MRS broth to 37 degrees Celsius. Then, we added 200 micro liters of the bacteria into the 300 mL of MRS Broth and we shall proceed with adding inulin and the powder of inulin and oligofructose together tomorrow 

]]> 2018-11-13 03:20:25 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/303556424 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/303556769 Errors: Emma said that there was supposed to be 200 microliters of Lactobacilli available for us, but when we extracted 200 microliters, there were still Lactobacilli left. Maybe there was a counting error. 

We took the Lactobacilli out of the Dante freezer, and it took us a while to actually utilize the bacteria and put it in the MRS Broth. This might have changed some aspects of the bacteria, putting it in undesirable conditions. ]]> 2018-11-13 03:22:33 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/303556769 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/303557140 -the machine to autoclave 
-lactobacilli
-test tubes autoclaved 
-micro pipette  
-larger item that was similar to a pipette (ask for specific name)
-MRS broth 
-300 ml container to put broth in 
-hot plate
-thermometer 

]]> 2018-11-13 03:24:39 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/303557140 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/304099909 -We spent 3 hours in lab today to pour the MRS broth and stock solution into the 40 test tubes and then we put different concentrations of inulin and the powder mixture of inulin and oligofructose in the 40 test tubes]]> 2018-11-14 01:45:43 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/304099909 amv2701 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/304100187 -lactobacilli
-mrs broth 
-test tubes
-spectrophotometer 
-cuvettes 
-weigh boats 
-scale 
-micropipettor 

]]> 2018-11-14 01:46:58 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/304100187 gracieonyia https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/304388376 We also measured the initial absorbances of the MRS Broth and the stock solution. ]]> 2018-11-14 16:29:21 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/304388376 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308060782 2018-11-26 23:07:22 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308060782 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308061209 2018-11-26 23:10:01 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308061209 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308061495 2018-11-26 23:11:44 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308061495 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308061872 The null hypothesis is that prebiotics have no effect on the probiotic growth. Our alternative hypotheses are that prebiotics would have a positive effect on the growth of Lactobacillus acidophilus and that using the pure inulin powder in the MRS broth would produce a more effective growth.]]> 2018-11-26 23:13:49 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308061872 seanvidac00 https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308062606 2018-11-26 23:17:24 UTC https://padlet.com/gracieonyia/mypbq7ioxxyy/wish/308062606