Disposable or Fixed-in-place stainless steel bioreactor?

-rocker platform uses the wave motion to liquid inside and this will allow the cells to get accessed to the nutrients needed to grow

2)stirred bioreactor

2) No cleaning

3) Increase plant flexibility

4) Contamination is exceedingly rare

5) Fast changeover resulting in greater batches/facility/yield

6) Reduced capital expenditure for equipment and facility

-featuring lesser parts in a fixed in place bioreactor hence it is less expensive to maintain on the mechanical end

2) Require ongoing consumable usage forecast

3) Quality by design - consider extractable and leachable study data

4) Relatively new technology

5) More responsibility for operators

6) Damage to single-use bags may cause leakage

Using Disposable bioreactors can help reduce the risk of cross-contamination and can help save time (shorter lead times). However, due to different shapes, agitation principles, and gassing strategies, scale-up and scale-down can be challenging.

With geometrical properties and long experience in process engineering knowledge, fixed-in-place bioreactors are still better than disposable bioreactors.

-pressure and full vacuum capability

-operate as closed system that requires little or no external environment

-an agitator system”, “A foam control system”, “Baffles system”, “PH & temperature control system”, “Fermenter vessel”, “Aeration system” and “An Impeller system”

-sparger, impeller

2) Inoculum, media, and buffers are aseptically transferred into the bag via a sterile tube fuser made of thermoplastics connection, resulting in exceptional strength

3) Significantly reduce the cost of validation and cleaning requirements

ADVANTAGE

1) Helps simplify downstream purification processes as fixed beds acts as a natural size exclusion filter for turbid biomolecules, reducing the need for further downstream clarification

2) Offers a fully closed, automated system of cell culture (lesser responsibility for operators), and contains a large surface area for cell attachment

3) Low shear force with homogenous microenvironment -> more concentrated harvest / product but less in volume -> simplified downstream process

https://www.biopharminternational.com/view/exploring-the-impact-of-fixed-bed-bioreactors-in-upstream-processing

For disposable bioreactor, it can be used in small scale cultivation like research in lab which does not require many samples. By having small scale bioreactor, it has its advantage which reduces cross-contamination and easy identification of cross-contamination origin. But it also has it disadvantages where it is not environmentally friendly and cannot get large scale results as time consuming to kept culturing cell lines in different disposable bioreactors.

In conclusion, though both bioreactors have its advantages and disadvantages, but the use of different bioreactors applies in different context like in lab research we only need a small-scale cell culture media thus reducing the wastage. In biopharma industry that grows cell in large batches, large scale bioreactor is thus more preferred.

2) Fed-batch culture

3) Continuous culture

processing time

microbial 12-24h

animal cells 5-7 days

Processing time

microbial 24-36h

animal cells 7-21 days