Phylum  :    Proteobacteria
Shigella belongs to the phylum Proteobacteria because it is gram-negative with the outer membrane mainly composed of lipopolysaccharides

Class      :    Gamma Proteobacteria
Shigella is classified in the class Gammaproteobacteria because it is comprised offacilitative anaerobic and fermentative gram negative bacteria.

Order      :    Enterobacteriales
Shigella fits into the Order Enterobacteriales because it is comprised of rod-shaped,facultative anaerobic, gram negative bacteria occurring as plant or animal parasites or assaprophytes. 

Family     :    Enterobacteriaceae
Shigella is classified into the family Enterobacteriaceae because members of this group grow well at 37 degrees Celsius, oxidase negative, and catalase positive, and reduces nitrates.

Genus      :    Shigella
Shigella fits into the genus Shigella because it only causes disease in primates and no other animals.

Species    :  S. dysenteriae
                     S. flexneri
                     S. boydii
                     S. sonnei
 Classified as Shigella  because it can be differentiated from the other types by positive β-D galactosidase and ornithine decarboxylase biochemical reactions.

> Drink clean water

Result: Shigella colonies are slightly pink and translucent, with or without rough edges ( being confirmed serologically and biochemically)

b) Salmonella Shigella Agar 

Result:
1.Salmonella will not ferment lactose but produce hydrogen sulfide (H₂S) gas.  The resulting bacterial colonies will appear colorless with black centers.
2. Shigella do not ferment lactose or produce hydrogen sulfide gas, so the resulting colonies will be colorless.
3.Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. They do not produce any hydrogen sulfide.

c) Hektoen enteric agar

Result:

Summary Result of Shigella biochemical test:
1.Gram-negative rods
2. Negative: H₂S, urease, glucose (gas), motility, lysine decarboxylase, sucrose, adonitol, inositol, lactose (2 days), KCN, malonate, citrate, and salicin
3.Positive: methyl red.

1) Several provisional serotypes have not been accepted by an international commission on the taxonomy of Shigella species. Resolve by referral to the U.S. Centers for Disease Control and Prevention (CDC), Atlanta, GA, or to the World Health Organization (WHO), Shigella sp. Reference Laboratories.

2) The cultures may be E. coli. Shigella species tend to be negative in acetate, citrate, and mucate media  reactions, whereas anaerobic E. coli tend to be positive in at least one of the reactions.