https://padlet.com/hanamel16_co/lvz9ruq8mi8j Made with fortitude en-us 2016-11-30 02:42:30 UTC 2025-12-23 08:36:29 UTC hello@padlet.com hanamel16_co https://padlet.com/hanamel16_co/lvz9ruq8mi8j/wish/140704286 -Studies in S. cerevisiae
shown that CAT2 encodes both peroxisomal and mito-
chondrial CAT isoforms.
-this CAT isoforms contain both an N-terminal
amphiphilic a-helix that acts as a mitochondrial targeting
signal and the tripeptide, AKL, a functional variant of the
type I peroxisomal targeting signal
-To determine the subcellular location of Pth2, Pth2–GFP fusion protein in M. 
grisea under the control of its native promoter is constructed & expressed.
-This gene 
fusion is fully functional and able to complement all 
Dpth2 mutant phenotypes 
-The expression of GFP was observed with a punctate distribution of 
vegetative hyphae, grown in axenic culture and then it is
transferred to medium containing 50 mM sodium 
acetate, and within conidia
-To investigate the 
cellular distribution, FoxA- 
RFP fusion protein is introduced to the same Pth2–GFP transformant 
of M. grisea. 
-Colocalization of the 
foxA-RFP and Pth2–GFP fusion proteins was observed 
in an intracellular vesicles
This result is observed when accumulated at the cortex 
of cells 
- the diffusion back- 
ground Pth2–GFP fluorescence in hyphae of the fungus is observed.
- As a conclusion, Pth2 is located in peroxisomes. The small cytosolic 
pool of the protein also possibly exists. ]]> 2016-11-30 02:43:19 UTC https://padlet.com/hanamel16_co/lvz9ruq8mi8j/wish/140704286