“Glamis hath murdered sleep, and therefore Cawdor

Shall sleep no more. Macbeth shall sleep no more.” (Shakespeare 2.2.41-43)

Macbeth starts having another one of his hallucinations because of the murder he committed, his hallucinations  always remind him of what he did, making him feel even more guilty to the point where he cant sleep peacefully anymore

"If chance will have me king, why, chance may

   crown me,

Without my stir." (Shakespeare 1.3.157-159)
Macbeth is talking to himself about the prophecy the witches had previously talked about. He doesn't want to take part in speeding up the process of him becoming king. He wants fate to runs its course.

One cried, “God bless us!” and “Amen” the other,

As they had seen me with these hangman’s hands.

List'ning their fear I could not say “Amen,”

When they did say “God bless us!” (Shakespeare 2.2.27-29) Macbeth said this quote in Act 2 to Lady Macbeth after he killed Duncan. He confessed to Lady Macbeth that when people discovered that Duncan's dead and started to pray and when they said "God bless us" he was unable to say Amen because of his guilt of killing Duncan.

I have no spur

To p,rick the sides of my intent, but only

Vaulting ambition, which o’erleaps itself

And falls on th’other. (Shakespeare 1.5.25-28)

In act 1 Scene 7 Macbeth tells duncan the he had no spur of intent but only his ambitions

“Glamis hath murdered sleep, and therefore Cawdor

Shall sleep no more. Macbeth shall sleep no more.” (Shakespeare 2.2.41-43) Macbeth's guilt prevents him from sleeping. Macbeth is murdering sleep.

“Glamis hath murdered sleep, and therefore Cawdor

Shall sleep no more. Macbeth shall sleep no more.” (Shakespeare 2.2.41-43)
He feels guilty for killing someone in there sleep and now he feels like when he sleeps he will dream about killings and death.

"Yet do I fear thy nature
It is too full o’ th’ milk of human kindness
To catch the nearest way. Thou wouldst be great,
Art not without ambition, but without
The illness should attend it"(Shakespeare 1.5.4-7).

"...fill me from the crown to the toe top-full

Of direst cruelty. Make thick my blood.

Stop up the access and passage to remorse,

that no compunctous visitings of nature 

Shake my fell purpose…" (Shakespeare 1.5.32-36)

Lady Macbeth had just learned about the prophecies from the witches about Macbeth being king, so she is asking spirits or demons to make her remorseless so she will be able to fulfill her ambition without regret.

THEME - AMBITION

I am afraid to think what I have done;

Look on ’t again I dare not. (Shakespeare 2.2.50-52)."

Macbeth says "I am afraid to think have I have done;..." This shows that he knows he did something wrong, he feels guilty because he is not proud of what he did. He is so full of guilt that he does not even want to think about what he did.

I’ll go no more:

I am afraid to think what I have done;

Look on ’t again I dare not. (Shakespeare 2.2.50-52).

THEME - GUILT

Macbeth cant accept what she has done and is living in fear and guilt.